eF-site ID 2y7c-ABCDE PDB Code 2y7c Chain A, B, C, D, E click to enlarge Title Atomic model of the Ocr-bound methylase complex from the Type I restriction-modification enzyme EcoKI (M2S1). Based on fitting into EM map 1534. Classification TRANSFERASE Compound TYPE-1 RESTRICTION ENZYME ECOKI SPECIFICITY PROTEIN Source ORGANISM_SCIENTIFIC: ESCHERICHIA COLI; Sequence A:  MSAGKLPEGWVIAPVSTVTTLIRGVTYKKEQAINYLKDDY LPLIRANNIQNGKFDTTDLVFVPKNLVKESQKISPEDIVI AMSSGSKSVVGKSAHQHLPFECSFGAFCGVLRPEKLIFSG FIAHFTKSSLYRNKISSLSAGANINNIKPASFDLINIPIP PLAEQKIIAEKLDTLLAQVDSTKARFEQIPQILKRFRQAV LGGAVNGKLTEKWRNFEPQHSVFKKLNFESILTELRNGLS SKPNESGVGHPILRISSVRAGHVDQNDIRFLECSESELNR HKLQDGDLLFTRYNGSLEFVGVCGLLKKLQHQNLLYPDKL IRARLTKDALPEYIEIFFSSPSARNAMMNCVKTTSGQKGI SGKDIKSQVVLLPPVKEQAEIVRRVEQLFAYADTIEKQVN NALARVNNLTQSILAKAFRGELTAQWRAENPDLISGENSA AALLEKIKAERAASGGKKASRKKS B:  MNNNDLVAKLWKLCDNLRDGGVSYQNYVNELASLLFLKMC KETGQEAEYLPEGYRWDDLKSRIGQEQLQFYRKMLVHLGE DDKKLVQAVFHNVSTTITEPKQITALVSNMDSLDWYNGAH GKSRDDFGDMYEGLLQKNANETKSGAGQYFTPRPLIKTII HLLKPQPREVVQDPAAGTAGFLIEADRYVKSQTNDLDDLD GDTQDFQIHRAFIGLELVPGTRRLALMNCLLHDIEGNLDH GGAIRLGNTLGSDGENLPKAHIVATNPPFGSAAGTNITRT FVHPTSNKQLCFMQHIIETLHPGGRAAVVVPDNVLFEGGK GTDIRRDLMDKCHLHTILRLPTGIFYAQGVKTNVLFFTKG TVANPNQDKNCTDDVWVYDLRTNMPSFGKRTPFTDEHLQP FERVYGEDPHGLSPRTEGEWSFNAEETEVADSEENKNTDQ HLATSRWRKFSREWIRTAKSDSLDISWLKDKDSIDADSLP EPDVLAAEAMGELVQALSELDALMRELGASDEADLQRQLL EEAFGGVKE C:  MNNNDLVAKLWKLCDNLRDGGVSYQNYVNELASLLFLKMC KETGQEAEYLPEGYRWDDLKSRIGQEQLQFYRKMLVHLGE DDKKLVQAVFHNVSTTITEPKQITALVSNMDSLDWYNGAH GKSRDDFGDMYEGLLQKNANETKSGAGQYFTPRPLIKTII HLLKPQPREVVQDPAAGTAGFLIEADRYVKSQTNDLDDLD GDTQDFQIHRAFIGLELVPGTRRLALMNCLLHDIEGNLDH GGAIRLGNTLGSDGENLPKAHIVATNPPFGSAAGTNITRT FVHPTSNKQLCFMQHIIETLHPGGRAAVVVPDNVLFEGGK GTDIRRDLMDKCHLHTILRLPTGIFYAQGVKTNVLFFTKG TVANPNQDKNCTDDVWVYDLRTNMPSFGKRTPFTDEHLQP FERVYGEDPHGLSPRTEGEWSFNAEETEVADSEENKNTDQ HLATSRWRKFSREWIRTAKSDSLDISWLKDKDSIDADSLP EPDVLAAEAMGELVQALSELDALMRELGASDEADLQRQLL EEAFGGVKE D:  MTYNNVFDHAYEMLKENIRYDDIRDTDDLHDAIHMAADNA VPHYYADIFSVMASEGIDLEFEDSGLMPDTKDVIRILQAR IYEQLTIDLWEDAEDLLNEYLEEVEEY E:  MTYNNVFDHAYEMLKENIRYDDIRDTDDLHDAIHMAADNA VPHYYADIFSVMASEGIDLEFEDSGLMPDTKDVIRILQAR IYEQLTIDLWEDAEDLLNEYLEEVEE Description (1)  TYPE-1 RESTRICTION ENZYME ECOKI SPECIFICITY PROTEIN (E.C.3.1.21.3), TYPE I RESTRICTION ENZYME ECOKI M PROTEIN (E.C.3.1.21.3, 2.1.1.72), GENE 0.3 PROTEIN Functional site 1) chain B residue 263-269 type prosite sequence VATNPPF description N6_MTASE N-6 Adenine-specific DNA methylases signature. VATNPPF source prosite : PS00092 2) chain B residue 148 type BINDING sequence Q description BINDING => ECO:0000250|UniProtKB:Q89Z59 source Swiss-Prot : SWS_FT_FI1 3) chain B residue 178 type BINDING sequence T description BINDING => ECO:0000250|UniProtKB:Q89Z59 source Swiss-Prot : SWS_FT_FI1 4) chain B residue 216 type BINDING sequence E description BINDING => ECO:0000250|UniProtKB:Q89Z59 source Swiss-Prot : SWS_FT_FI1 5) chain C residue 148 type BINDING sequence Q description BINDING => ECO:0000250|UniProtKB:Q89Z59 source Swiss-Prot : SWS_FT_FI1 6) chain C residue 178 type BINDING sequence T description BINDING => ECO:0000250|UniProtKB:Q89Z59 source Swiss-Prot : SWS_FT_FI1 7) chain C residue 216 type BINDING sequence E description BINDING => ECO:0000250|UniProtKB:Q89Z59 source Swiss-Prot : SWS_FT_FI1

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