eF-site ID 2xfg-AB PDB Code 2xfg Chain A, B click to enlarge Title Reassembly and co-crystallization of a family 9 processive endoglucanase from separately expressed GH9 and CBM3c modules Classification HYDROLASE/SUGAR BINDING PROTEIN Compound ENDOGLUCANASE 1 Source Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum) (GUNI_CLOTH) Sequence A:  TGAFNYGEALQKAIFFYECQRSGKLDSSTLRLNWRGDSGL DDGKDAGIDLTGGWYDAGDHVKFNLPMSYSAAMLGWAVYE YEDAFKQSGQYNHILNNIKWACDYFIKCHPEKDVYYYQVG DGHADHAWWGPAEVMPMERPSYKVDRSSPGSTVVAETSAA LAIASIIFKKVDGEYSKECLKHAKELFEFADTTKSDDGYT AANGFYNSWSGFYDELSWAAVWLYLATNDSSYLDKAESYS DKWGYEPQTNIPKYKWAQCWDDVTYGTYLLLARIKNDNGK YKEAIERHLDWWTTGYNGERITYTPKGLAWLDQWGSLRYA TTTAFLACVYSDWENGDKEKAKTYLEFARSQADYALGSTG RSFVVGFGENPPKRPHHRTAHGSWADSQMEPPEHRHVLYG ALVGGPDSTDNYTDDISNYTCNEVACDYNAGFVGLLAKMY KLYGEL B:  GSPDPKFNGIEEVPEDEIFVEAGVNASGNNFIEIKAIVNN KSGWPARVCENLSFRYFINIEEIVNAGKSASDLQVSSSYN QGAKLSDVKHYKDNIYYVEVDLSGTKIYPGGQSAYKKEVQ FRISAPEGTVFNPENDYSYQGLSAGTVVKSEYIPVYDAGV LVFGREPLEHHH Description Functional site 1) chain A residue 210 type sequence S description BINDING SITE FOR RESIDUE CA A 1447 source : AC1 2) chain A residue 211 type sequence G description BINDING SITE FOR RESIDUE CA A 1447 source : AC1 3) chain A residue 214 type sequence D description BINDING SITE FOR RESIDUE CA A 1447 source : AC1 4) chain A residue 215 type sequence E description BINDING SITE FOR RESIDUE CA A 1447 source : AC1 5) chain A residue 261 type sequence D description BINDING SITE FOR RESIDUE CA A 1447 source : AC1 6) chain B residue 505 type sequence N description BINDING SITE FOR RESIDUE CA B 1619 source : AC2 7) chain B residue 508 type sequence E description BINDING SITE FOR RESIDUE CA B 1619 source : AC2 8) chain B residue 578 type sequence N description BINDING SITE FOR RESIDUE CA B 1619 source : AC2 9) chain B residue 581 type sequence N description BINDING SITE FOR RESIDUE CA B 1619 source : AC2 10) chain B residue 582 type sequence D description BINDING SITE FOR RESIDUE CA B 1619 source : AC2 11) chain A residue 81 type sequence Y description BINDING SITE FOR RESIDUE CL A 1448 source : AC3 12) chain A residue 83 type sequence D description BINDING SITE FOR RESIDUE CL A 1448 source : AC3 13) chain A residue 84 type sequence A description BINDING SITE FOR RESIDUE CL A 1448 source : AC3 14) chain A residue 441 type sequence K description BINDING SITE FOR RESIDUE CL A 1448 source : AC3 15) chain A residue 11 type sequence Q description BINDING SITE FOR RESIDUE CL A 1449 source : AC4 16) chain A residue 90 type sequence Q description BINDING SITE FOR RESIDUE CL A 1449 source : AC4 17) chain A residue 93 type sequence H description BINDING SITE FOR RESIDUE CL A 1449 source : AC4 18) chain A residue 207 type sequence N description BINDING SITE FOR RESIDUE CL A 1450 source : AC5 19) chain A residue 208 type sequence S description BINDING SITE FOR RESIDUE CL A 1450 source : AC5 20) chain B residue 538 type sequence K description BINDING SITE FOR RESIDUE CL A 1450 source : AC5 21) chain A residue 414 type ACT_SITE sequence D description ACT_SITE => ECO:0000255|PROSITE-ProRule:PRU10060 source Swiss-Prot : SWS_FT_FI3 22) chain A residue 423 type ACT_SITE sequence E description ACT_SITE => ECO:0000255|PROSITE-ProRule:PRU10060 source Swiss-Prot : SWS_FT_FI3 23) chain A residue 354-378 type prosite sequence YALGSTGRSFVVGFGENPPKRPHHR description GH9_2 Glycosyl hydrolases family 9 (GH9) active site signature 2. YALGsTgr..SFVVGf....GenPPkrPHHR source prosite : PS00592 24) chain A residue 412-430 type prosite sequence YTDDISNYTCNEVACDYNA description GH9_3 Glycosyl hydrolases family 9 (GH9) active site signature 3. YtDdisnYtcnEvAcdyNA source prosite : PS00698 25) chain A residue 50-67 type prosite sequence LTGGWYDAGDHVKFNLPM description GH9_1 Glycosyl hydrolases family 9 (GH9) active site signature 1. LtGGWYDAGDhvKFnLPM source prosite : PS60032 26) chain A residue 59 type ACT_SITE sequence D description Nucleophile => ECO:0000255|PROSITE-ProRule:PRU10140 source Swiss-Prot : SWS_FT_FI1 27) chain A residue 376 type ACT_SITE sequence H description ACT_SITE => ECO:0000255|PROSITE-ProRule:PRU10059 source Swiss-Prot : SWS_FT_FI2

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