eF-site ID 2w5e-ABCDEF PDB Code 2w5e Chain A, B, C, D, E, F click to enlarge Title Structural and biochemical analysis of human pathogenic astrovirus serine protease at 2.0 Angstrom resolution Classification HYDROLASE Compound PUTATIVE SERINE PROTEASE Source Human astrovirus-1 (HAstV-1) (Q3ZN04_HASV1) Sequence A:  KHHHHHHIKPGALCVIDTPEGKGTGFFSGNDIVTAAHVVG NNTFVNVCYEGLMYEAKVRYMPEKDIAFITCPGDLHPTAR LKLSKNPDYSCVTVMAYVNEDLVVSTAAAMVYGNTLSYAV RTQDGMSGAPVCDKYCRVLAVHQTNTGYTGGAVIIDPTDF HP B:  KHHHHHHIKPGALCVIDTPEGKGTGFFSGNDIVTAAHVVG NNTFVNVCYEGLMYEAKVRYMPEKDIAFITCPGDLHPTAR LKLSKNPDYSCVTVMAYVNEDLVVSTAAAMVYGNTLSYAV RTQDGMSGAPVCDKYCRVLAVHQTNTGYTGGAVIIDPTDF HP C:  KHHHHHHIKPGALCVIDTPEGKGTGFFSGNDIVTAAHVVG NNTFVNVCYEGLMYEAKVRYMPEKDIAFITCPGDLHPTAR LKLSKNPDYSCVTVMAYVNEDLVVSTAAAMVYGNTLSYAV RTQDGMSGAPVCDKYCRVLAVHQTNTGYTGGAVIIDPTDF HP D:  KHHHHHHIKPGALCVIDTPEGKGTGFFSGNDIVTAAHVVG NNTFVNVCYEGLMYEAKVRYMPEKDIAFITCPGDLHPTAR LKLSKNPDYSCVTVMAYVNEDLVVSTAAAMVYGNTLSYAV RTQDGMSGAPVCDKYCRVLAVHQTNTGYTGGAVIIDPTDF HP E:  KHHHHHHIKPGALCVIDTPEGKGTGFFSGNDIVTAAHVVG NNTFVNVCYEGLMYEAKVRYMPEKDIAFITCPGDLHPTAR LKLSKNPDYSCVTVMAYVNEDLVVSTAAAMVYGNTLSYAV RTQDGMSGAPVCDKYCRVLAVHQTNTGYTGGAVIIDPTDF HP F:  KHHHHHHIKPGALCVIDTPEGKGTGFFSGNDIVTAAHVVG NNTFVNVCYEGLMYEAKVRYMPEKDIAFITCPGDLHPTAR LKLSKNPDYSCVTVMAYVNEDLVVSTAAAMVYGNTLSYAV RTQDGMSGAPVCDKYCRVLAVHQTNTGYTGGAVIIDPTDF HP Description Functional site 1) chain A residue -5 type sequence H description BINDING SITE FOR RESIDUE CD A 1587 source : AC1 2) chain A residue -3 type sequence H description BINDING SITE FOR RESIDUE CD A 1587 source : AC1 3) chain A residue 515 type sequence C description BINDING SITE FOR RESIDUE CD A 1587 source : AC1 4) chain A residue -5 type sequence H description BINDING SITE FOR RESIDUE CL A 1588 source : AC2 5) chain A residue -4 type sequence H description BINDING SITE FOR RESIDUE CL A 1588 source : AC2 6) chain A residue -3 type sequence H description BINDING SITE FOR RESIDUE CL A 1588 source : AC2 7) chain A residue -2 type sequence H description BINDING SITE FOR RESIDUE CD A 1589 source : AC3 8) chain A residue -4 type sequence H description BINDING SITE FOR RESIDUE CD A 1589 source : AC3 9) chain B residue 474 type sequence E description BINDING SITE FOR RESIDUE CD A 1589 source : AC3 10) chain F residue 500 type sequence H description BINDING SITE FOR RESIDUE CD A 1589 source : AC3 11) chain A residue 514 type sequence S description BINDING SITE FOR RESIDUE CL A 1590 source : AC4 12) chain A residue 515 type sequence C description BINDING SITE FOR RESIDUE CL A 1590 source : AC4 13) chain B residue 472 type sequence C description BINDING SITE FOR RESIDUE CD A 1591 source : AC5 14) chain B residue 477 type sequence M description BINDING SITE FOR RESIDUE CD A 1591 source : AC5 15) chain A residue -6 type sequence H description BINDING SITE FOR RESIDUE CL A 1592 source : AC6 16) chain B residue 472 type sequence C description BINDING SITE FOR RESIDUE CL A 1592 source : AC6 17) chain B residue 446 type sequence K description BINDING SITE FOR RESIDUE CL B 1587 source : AC7 18) chain B residue -5 type sequence H description BINDING SITE FOR RESIDUE CD B 1588 source : AC8 19) chain B residue -3 type sequence H description BINDING SITE FOR RESIDUE CD B 1588 source : AC8 20) chain B residue 515 type sequence C description BINDING SITE FOR RESIDUE CD B 1588 source : AC8 21) chain B residue -3 type sequence H description BINDING SITE FOR RESIDUE CL B 1589 source : AC9 22) chain B residue -4 type sequence H description BINDING SITE FOR RESIDUE CL B 1589 source : AC9 23) chain C residue 500 type sequence H description BINDING SITE FOR RESIDUE CL B 1589 source : AC9 24) chain A residue 474 type sequence E description BINDING SITE FOR RESIDUE CD B 1590 source : BC1 25) chain B residue -4 type sequence H description BINDING SITE FOR RESIDUE CD B 1590 source : BC1 26) chain B residue -2 type sequence H description BINDING SITE FOR RESIDUE CD B 1590 source : BC1 27) chain C residue 500 type sequence H description BINDING SITE FOR RESIDUE CD B 1590 source : BC1 28) chain A residue 472 type sequence C description BINDING SITE FOR RESIDUE CL B 1591 source : BC2 29) chain B residue -6 type sequence H description BINDING SITE FOR RESIDUE CL B 1591 source : BC2 30) chain A residue 472 type sequence C description BINDING SITE FOR RESIDUE CD A 1593 source : BC3 31) chain B residue -6 type sequence H description BINDING SITE FOR RESIDUE CD A 1593 source : BC3 32) chain B residue -7 type sequence K description BINDING SITE FOR RESIDUE CD A 1593 source : BC3 33) chain A residue 472 type sequence C description BINDING SITE FOR RESIDUE CL A 1594 source : BC4 34) chain A residue 477 type sequence M description BINDING SITE FOR RESIDUE CL A 1594 source : BC4 35) chain B residue 514 type sequence S description BINDING SITE FOR RESIDUE CL B 1592 source : BC5 36) chain B residue 515 type sequence C description BINDING SITE FOR RESIDUE CL B 1592 source : BC5 37) chain C residue -4 type sequence H description BINDING SITE FOR RESIDUE CD C 1587 source : BC6 38) chain C residue -2 type sequence H description BINDING SITE FOR RESIDUE CD C 1587 source : BC6 39) chain D residue 474 type sequence E description BINDING SITE FOR RESIDUE CD C 1587 source : BC6 40) chain E residue 500 type sequence H description BINDING SITE FOR RESIDUE CD C 1587 source : BC6 41) chain C residue -5 type sequence H description BINDING SITE FOR RESIDUE CL C 1588 source : BC7 42) chain C residue -4 type sequence H description BINDING SITE FOR RESIDUE CL C 1588 source : BC7 43) chain C residue -3 type sequence H description BINDING SITE FOR RESIDUE CL C 1588 source : BC7 44) chain C residue -3 type sequence H description BINDING SITE FOR RESIDUE CD C 1589 source : BC8 45) chain C residue -5 type sequence H description BINDING SITE FOR RESIDUE CD C 1589 source : BC8 46) chain C residue 515 type sequence C description BINDING SITE FOR RESIDUE CD C 1589 source : BC8 47) chain C residue 514 type sequence S description BINDING SITE FOR RESIDUE CL C 1590 source : BC9 48) chain C residue 515 type sequence C description BINDING SITE FOR RESIDUE CL C 1590 source : BC9 49) chain C residue -6 type sequence H description BINDING SITE FOR RESIDUE CL C 1591 source : CC1 50) chain D residue 472 type sequence C description BINDING SITE FOR RESIDUE CL C 1591 source : CC1 51) chain C residue -6 type sequence H description BINDING SITE FOR RESIDUE CD C 1592 source : CC2 52) chain D residue 472 type sequence C description BINDING SITE FOR RESIDUE CD C 1592 source : CC2 53) chain D residue 439 type sequence V description BINDING SITE FOR RESIDUE CL D 1587 source : CC3 54) chain D residue 446 type sequence K description BINDING SITE FOR RESIDUE CL D 1587 source : CC3 55) chain D residue 472 type sequence C description BINDING SITE FOR RESIDUE CL D 1587 source : CC3 56) chain D residue 433 type sequence K description BINDING SITE FOR RESIDUE CL D 1588 source : CC4 57) chain D residue 434 type sequence P description BINDING SITE FOR RESIDUE CL D 1588 source : CC4 58) chain D residue 435 type sequence G description BINDING SITE FOR RESIDUE CL D 1588 source : CC4 59) chain A residue 500 type sequence H description BINDING SITE FOR RESIDUE CD D 1589 source : CC5 60) chain C residue 474 type sequence E description BINDING SITE FOR RESIDUE CD D 1589 source : CC5 61) chain D residue -2 type sequence H description BINDING SITE FOR RESIDUE CD D 1589 source : CC5 62) chain D residue -4 type sequence H description BINDING SITE FOR RESIDUE CD D 1589 source : CC5 63) chain D residue -3 type sequence H description BINDING SITE FOR RESIDUE CL D 1590 source : CC6 64) chain D residue -5 type sequence H description BINDING SITE FOR RESIDUE CD D 1591 source : CC7 65) chain D residue -3 type sequence H description BINDING SITE FOR RESIDUE CD D 1591 source : CC7 66) chain D residue 515 type sequence C description BINDING SITE FOR RESIDUE CD D 1591 source : CC7 67) chain D residue 514 type sequence S description BINDING SITE FOR RESIDUE CL D 1592 source : CC8 68) chain D residue 515 type sequence C description BINDING SITE FOR RESIDUE CL D 1592 source : CC8 69) chain C residue 446 type sequence K description BINDING SITE FOR RESIDUE CL C 1593 source : CC9 70) chain C residue 472 type sequence C description BINDING SITE FOR RESIDUE CD C 1594 source : DC1 71) chain D residue -6 type sequence H description BINDING SITE FOR RESIDUE CD C 1594 source : DC1 72) chain C residue 472 type sequence C description BINDING SITE FOR RESIDUE CL D 1593 source : DC2 73) chain D residue -6 type sequence H description BINDING SITE FOR RESIDUE CL D 1593 source : DC2 74) chain C residue 433 type sequence K description BINDING SITE FOR RESIDUE CL C 1595 source : DC3 75) chain C residue 434 type sequence P description BINDING SITE FOR RESIDUE CL C 1595 source : DC3 76) chain C residue 435 type sequence G description BINDING SITE FOR RESIDUE CL C 1595 source : DC3 77) chain B residue 500 type sequence H description BINDING SITE FOR RESIDUE CD E 1587 source : DC4 78) chain E residue -2 type sequence H description BINDING SITE FOR RESIDUE CD E 1587 source : DC4 79) chain E residue -4 type sequence H description BINDING SITE FOR RESIDUE CD E 1587 source : DC4 80) chain F residue 474 type sequence E description BINDING SITE FOR RESIDUE CD E 1587 source : DC4 81) chain B residue 500 type sequence H description BINDING SITE FOR RESIDUE CL E 1588 source : DC5 82) chain E residue -3 type sequence H description BINDING SITE FOR RESIDUE CL E 1588 source : DC5 83) chain E residue -4 type sequence H description BINDING SITE FOR RESIDUE CL E 1588 source : DC5 84) chain E residue 514 type sequence S description BINDING SITE FOR RESIDUE CL E 1589 source : DC6 85) chain E residue 515 type sequence C description BINDING SITE FOR RESIDUE CL E 1589 source : DC6 86) chain E residue -3 type sequence H description BINDING SITE FOR RESIDUE CD E 1590 source : DC7 87) chain E residue -5 type sequence H description BINDING SITE FOR RESIDUE CD E 1590 source : DC7 88) chain E residue 515 type sequence C description BINDING SITE FOR RESIDUE CD E 1590 source : DC7 89) chain E residue -6 type sequence H description BINDING SITE FOR RESIDUE CL E 1591 source : DC8 90) chain F residue 472 type sequence C description BINDING SITE FOR RESIDUE CL E 1591 source : DC8 91) chain E residue -6 type sequence H description BINDING SITE FOR RESIDUE CD E 1592 source : DC9 92) chain F residue 472 type sequence C description BINDING SITE FOR RESIDUE CD E 1592 source : DC9 93) chain F residue 472 type sequence C description BINDING SITE FOR RESIDUE CL F 1587 source : EC1 94) chain F residue 477 type sequence M description BINDING SITE FOR RESIDUE CL F 1587 source : EC1 95) chain F residue 433 type sequence K description BINDING SITE FOR RESIDUE CL F 1588 source : EC2 96) chain F residue 434 type sequence P description BINDING SITE FOR RESIDUE CL F 1588 source : EC2 97) chain F residue 435 type sequence G description BINDING SITE FOR RESIDUE CL F 1588 source : EC2 98) chain D residue 500 type sequence H description BINDING SITE FOR RESIDUE CL F 1589 source : EC3 99) chain F residue -4 type sequence H description BINDING SITE FOR RESIDUE CL F 1589 source : EC3 100) chain F residue -3 type sequence H description BINDING SITE FOR RESIDUE CL F 1589 source : EC3 101) chain D residue 500 type sequence H description BINDING SITE FOR RESIDUE CD F 1590 source : EC4 102) chain E residue 474 type sequence E description BINDING SITE FOR RESIDUE CD F 1590 source : EC4 103) chain F residue -2 type sequence H description BINDING SITE FOR RESIDUE CD F 1590 source : EC4 104) chain F residue -4 type sequence H description BINDING SITE FOR RESIDUE CD F 1590 source : EC4 105) chain E residue 472 type sequence C description BINDING SITE FOR RESIDUE CL F 1591 source : EC5 106) chain E residue 477 type sequence M description BINDING SITE FOR RESIDUE CL F 1591 source : EC5 107) chain F residue -3 type sequence H description BINDING SITE FOR RESIDUE CD F 1592 source : EC6 108) chain F residue -5 type sequence H description BINDING SITE FOR RESIDUE CD F 1592 source : EC6 109) chain F residue 515 type sequence C description BINDING SITE FOR RESIDUE CD F 1592 source : EC6 110) chain E residue 472 type sequence C description BINDING SITE FOR RESIDUE CL F 1593 source : EC7 111) chain F residue -6 type sequence H description BINDING SITE FOR RESIDUE CL F 1593 source : EC7 112) chain E residue 472 type sequence C description BINDING SITE FOR RESIDUE CD F 1594 source : EC8 113) chain F residue -6 type sequence H description BINDING SITE FOR RESIDUE CD F 1594 source : EC8 114) chain F residue -7 type sequence K description BINDING SITE FOR RESIDUE CD F 1594 source : EC8 115) chain F residue 514 type sequence S description BINDING SITE FOR RESIDUE CL F 1595 source : EC9 116) chain F residue 515 type sequence C description BINDING SITE FOR RESIDUE CL F 1595 source : EC9 117) chain E residue 433 type sequence K description BINDING SITE FOR RESIDUE CL E 1593 source : FC1 118) chain E residue 434 type sequence P description BINDING SITE FOR RESIDUE CL E 1593 source : FC1 119) chain E residue 435 type sequence G description BINDING SITE FOR RESIDUE CL E 1593 source : FC1 120) chain A residue 461 type ACT_SITE sequence H description Charge relay system; for serine protease activity => ECO:0000250 source Swiss-Prot : SWS_FT_FI1 121) chain D residue 461 type ACT_SITE sequence H description Charge relay system; for serine protease activity => ECO:0000250 source Swiss-Prot : SWS_FT_FI1 122) chain D residue 489 type ACT_SITE sequence D description Charge relay system; for serine protease activity => ECO:0000250 source Swiss-Prot : SWS_FT_FI1 123) chain D residue 551 type ACT_SITE sequence S description Charge relay system; for serine protease activity => ECO:0000250 source Swiss-Prot : SWS_FT_FI1 124) chain E residue 461 type ACT_SITE sequence H description Charge relay system; for serine protease activity => ECO:0000250 source Swiss-Prot : SWS_FT_FI1 125) chain E residue 489 type ACT_SITE sequence D description Charge relay system; for serine protease activity => ECO:0000250 source Swiss-Prot : SWS_FT_FI1 126) chain E residue 551 type ACT_SITE sequence S description Charge relay system; for serine protease activity => ECO:0000250 source Swiss-Prot : SWS_FT_FI1 127) chain F residue 461 type ACT_SITE sequence H description Charge relay system; for serine protease activity => ECO:0000250 source Swiss-Prot : SWS_FT_FI1 128) chain F residue 489 type ACT_SITE sequence D description Charge relay system; for serine protease activity => ECO:0000250 source Swiss-Prot : SWS_FT_FI1 129) chain F residue 551 type ACT_SITE sequence S description Charge relay system; for serine protease activity => ECO:0000250 source Swiss-Prot : SWS_FT_FI1 130) chain A residue 489 type ACT_SITE sequence D description Charge relay system; for serine protease activity => ECO:0000250 source Swiss-Prot : SWS_FT_FI1 131) chain A residue 551 type ACT_SITE sequence S description Charge relay system; for serine protease activity => ECO:0000250 source Swiss-Prot : SWS_FT_FI1 132) chain B residue 461 type ACT_SITE sequence H description Charge relay system; for serine protease activity => ECO:0000250 source Swiss-Prot : SWS_FT_FI1 133) chain B residue 489 type ACT_SITE sequence D description Charge relay system; for serine protease activity => ECO:0000250 source Swiss-Prot : SWS_FT_FI1 134) chain B residue 551 type ACT_SITE sequence S description Charge relay system; for serine protease activity => ECO:0000250 source Swiss-Prot : SWS_FT_FI1 135) chain C residue 461 type ACT_SITE sequence H description Charge relay system; for serine protease activity => ECO:0000250 source Swiss-Prot : SWS_FT_FI1 136) chain C residue 489 type ACT_SITE sequence D description Charge relay system; for serine protease activity => ECO:0000250 source Swiss-Prot : SWS_FT_FI1 137) chain C residue 551 type ACT_SITE sequence S description Charge relay system; for serine protease activity => ECO:0000250 source Swiss-Prot : SWS_FT_FI1

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