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eF-site ID
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2vik-A |
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PDB Code
|
2vik |
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Chain
|
A |
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click to enlarge
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Title
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REFINED STRUCTURE OF THE ACTIN-SEVERING DOMAIN VILLIN 14T, DETERMINED BY SOLUTION NMR, MINIMIZED AVERAGE STRUCTURE |
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Classification
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ACTIN-BINDING PROTEIN |
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Compound
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VILLIN 14T |
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Source
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Gallus gallus (Chicken) (VILI_CHICK) |
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Sequence
|
A: |
VELSKKVTGKLDKTTPGIQIWRIENMEMVPVPTKSYGNFY
EGDCYVLLSTRKTGSGFSYNIHYWLGKNSSQDEQGAAAIY
TTQMDEYLGSVAVQHREVQGHESETFRAYFKQGLIYKQGG
VASGMK
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Description
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(1) |
VILLIN 14T
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Functional site
|
|
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1)
|
chain |
A |
| residue |
79 |
| type |
|
| sequence |
I
|
| description |
CENTER OF ACTIN MONOMER BINDING SITE, BASED ON COMPARISON WITH THE GELSOLIN SEGMENT 1-ACTIN COCRYSTAL (CENTERED ON ILE 79).
|
| source |
: ACT
|
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2)
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chain |
A |
| residue |
43 |
| type |
|
| sequence |
D
|
| description |
SIDE CHAINS THAT PROVIDE LIGANDS TO CALCIUM IN THE STRONGER BINDING SITE, BASED ON COMPARISON TO GELSOLIN SEGMENT 1 AND CALCIUM TITRATIONS MONITORED BY NMR. NOTE THIS CORRESPONDS TO THE INTRAMOLECULAR SITE IN THE GELSOLIN SEGMENT 1-ACTIN CO-CRYSTAL (ASP 43, GLU 73).
|
| source |
: CA1
|
|
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3)
|
chain |
A |
| residue |
73 |
| type |
|
| sequence |
E
|
| description |
SIDE CHAINS THAT PROVIDE LIGANDS TO CALCIUM IN THE STRONGER BINDING SITE, BASED ON COMPARISON TO GELSOLIN SEGMENT 1 AND CALCIUM TITRATIONS MONITORED BY NMR. NOTE THIS CORRESPONDS TO THE INTRAMOLECULAR SITE IN THE GELSOLIN SEGMENT 1-ACTIN CO-CRYSTAL (ASP 43, GLU 73).
|
| source |
: CA1
|
|
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4)
|
chain |
A |
| residue |
85 |
| type |
|
| sequence |
D
|
| description |
SIDE CHAIN THAT PROVIDES LIGANDS TO CALCIUM IN THE WEAKER BINDING SITE, BASED ON COMPARISON TO GELSOLIN SEGMENT 1 AND CALCIUM TITRATIONS MONITORED BY NMR. NOTE THIS CORRESPONDS TO THE INTERMOLECULAR SITE IN THE GELSOLIN SEGMENT 1-ACTIN CO-CRYSTAL (ASP 85).
|
| source |
: CA2
|
|
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5)
|
chain |
A |
| residue |
111 |
| type |
BINDING |
| sequence |
K
|
| description |
BINDING => ECO:0000250
|
| source |
Swiss-Prot : SWS_FT_FI1
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