|
eF-site ID
|
2ven-A |
PDB Code
|
2ven |
Chain
|
A |
|
click to enlarge
|
|
Title
|
Structure-based enzyme engineering efforts with an inactive monomeric TIM variant: the importance of a single point mutation for generating an active site with suitable binding properties |
Classification
|
ISOMERASE |
Compound
|
GLYCOSOMAL TRIOSEPHOSPHATE ISOMERASE |
Source
|
(TPIS_TRYBB) |
|
Sequence
|
A: |
SKPQPIAAANWKSLSELIDLFNSTSINHDVQCVVASTFVH
LAMTKERLSHPKFVIAAQNAGNADALASLKDFGVNWIVLG
HSERRWYYGETNEIVADKVAAAVASGFMVIACIGETLQER
ESGRTAVVVLTQIAAIAKKLKKADWAKVVIAYEPVWKVAT
PQQAQEAHALIRSWVSSKIGADVAGELRILYGGSVNGKNA
RTLYQQRDVNGFLAGLKPEFVDIIKATQ
|
|
Description
|
|
Functional site
|
|
1)
|
chain |
A |
residue |
103 |
type |
ACT_SITE |
sequence |
G
|
description |
Electrophile
|
source |
Swiss-Prot : SWS_FT_FI1
|
|
2)
|
chain |
A |
residue |
11 |
type |
BINDING |
sequence |
N
|
description |
|
source |
Swiss-Prot : SWS_FT_FI3
|
|
3)
|
chain |
A |
residue |
13 |
type |
BINDING |
sequence |
K
|
description |
|
source |
Swiss-Prot : SWS_FT_FI3
|
|
|
|