eF-site ID 2vel-B PDB Code 2vel Chain B click to enlarge Title Structure-based enzyme engineering efforts with an inactive monomeric TIM variant: the importance of a single point mutation for generating an active site with suitable binding properties. Classification ISOMERASE Compound GLYCOSOMAL TRIOSEPHOSPHATE ISOMERASE Source (TPIS_TRYBB) Sequence B:  SKPQPIAAANWKSGSPDSLSELIDLFNSTSINHDVQCVVA STFVHLAMTKERLSHPKFVIAAQNAGNADALASLKDFGVN WIVLGHSERRWYYGETNEIVADKVAAAVASGFMVIACIGE TLQERESGRTAVVVLTQIAAIAKKLKKADWAKVVIAYEPV WAIGTGKVATPQQAQEAHALIRSWVSSKIGADVAGELRIL YGGSVNGKNARTLYQQRDVNGFLAGLKPEFVDIIKATQ Description Functional site 1) chain B residue 98 type sequence R description BINDING SITE FOR RESIDUE CL B1251 source : AC2 2) chain B residue 102 type sequence Y description BINDING SITE FOR RESIDUE CL B1251 source : AC2 3) chain B residue 11 type sequence N description BINDING SITE FOR RESIDUE PGA B1252 source : AC3 4) chain B residue 13 type sequence K description BINDING SITE FOR RESIDUE PGA B1252 source : AC3 5) chain B residue 95 type sequence H description BINDING SITE FOR RESIDUE PGA B1252 source : AC3 6) chain B residue 167 type sequence E description BINDING SITE FOR RESIDUE PGA B1252 source : AC3 7) chain B residue 171 type sequence A description BINDING SITE FOR RESIDUE PGA B1252 source : AC3 8) chain B residue 172 type sequence I description BINDING SITE FOR RESIDUE PGA B1252 source : AC3 9) chain B residue 173 type sequence G description BINDING SITE FOR RESIDUE PGA B1252 source : AC3 10) chain B residue 213 type sequence S description BINDING SITE FOR RESIDUE PGA B1252 source : AC3 11) chain B residue 232 type sequence L description BINDING SITE FOR RESIDUE PGA B1252 source : AC3 12) chain B residue 233 type sequence A description BINDING SITE FOR RESIDUE PGA B1252 source : AC3 13) chain B residue 234 type sequence G description BINDING SITE FOR RESIDUE PGA B1252 source : AC3 14) chain B residue 239 type sequence K description BINDING SITE FOR RESIDUE PGA B1252 source : AC3 15) chain B residue 95 type ACT_SITE sequence H description Electrophile source Swiss-Prot : SWS_FT_FI1 16) chain B residue 167 type ACT_SITE sequence E description Proton acceptor source Swiss-Prot : SWS_FT_FI2 17) chain B residue 11 type BINDING sequence N description source Swiss-Prot : SWS_FT_FI3 18) chain B residue 13 type BINDING sequence K description source Swiss-Prot : SWS_FT_FI3

Display surface Download Links