eF-site ID 2vek-B PDB Code 2vek Chain B click to enlarge Title Structure-based enzyme engineering efforts with an inactive monomeric TIM variant: the importance of a single point mutation for generating an active site with suitable binding properties Classification ISOMERASE Compound TRIOSEPHOSPHATE ISOMERASE Source Trypanosoma brucei brucei (TPIS_TRYBB) Sequence B:  SKPQPIAAANWKSGSPDSLSELIDLFNSTSINHDVQCVVA STFVHLAMTKERLSHPKFVIAAQNAGNADALASLKDFGVN WIVLGHSERRWYYGETNEIVADKVAAAVASGFMVIACIGE TLQERESGRTAVVVLTQIAAIAKKLKKADWAKVVIAYEPV WAIGTGKVATPQQAQEAHALIRSWVSSKIGADVAGELRIL YGGSVNGKNARTLYQQRDVNGFLAGLKPEFVDIIKATQ Description Functional site 1) chain B residue 11 type sequence N description BINDING SITE FOR RESIDUE CIT B1251 source : AC2 2) chain B residue 13 type sequence K description BINDING SITE FOR RESIDUE CIT B1251 source : AC2 3) chain B residue 95 type sequence H description BINDING SITE FOR RESIDUE CIT B1251 source : AC2 4) chain B residue 167 type sequence E description BINDING SITE FOR RESIDUE CIT B1251 source : AC2 5) chain B residue 171 type sequence A description BINDING SITE FOR RESIDUE CIT B1251 source : AC2 6) chain B residue 172 type sequence I description BINDING SITE FOR RESIDUE CIT B1251 source : AC2 7) chain B residue 173 type sequence G description BINDING SITE FOR RESIDUE CIT B1251 source : AC2 8) chain B residue 213 type sequence S description BINDING SITE FOR RESIDUE CIT B1251 source : AC2 9) chain B residue 233 type sequence A description BINDING SITE FOR RESIDUE CIT B1251 source : AC2 10) chain B residue 234 type sequence G description BINDING SITE FOR RESIDUE CIT B1251 source : AC2 11) chain B residue 239 type sequence K description BINDING SITE FOR RESIDUE CIT B1251 source : AC2 12) chain B residue 29 type sequence N description BINDING SITE FOR RESIDUE TBU B1252 source : AC3 13) chain B residue 30 type sequence S description BINDING SITE FOR RESIDUE TBU B1252 source : AC3 14) chain B residue 31 type sequence T description BINDING SITE FOR RESIDUE TBU B1252 source : AC3 15) chain B residue 32 type sequence S description BINDING SITE FOR RESIDUE TBU B1252 source : AC3 16) chain B residue 193 type sequence W description BINDING SITE FOR RESIDUE TBU B1252 source : AC3 17) chain B residue 197 type sequence K description BINDING SITE FOR RESIDUE TBU B1252 source : AC3 18) chain B residue 31 type sequence T description BINDING SITE FOR RESIDUE ASF B1253 source : AC4 19) chain B residue 32 type sequence S description BINDING SITE FOR RESIDUE ASF B1253 source : AC4 20) chain B residue 33 type sequence I description BINDING SITE FOR RESIDUE ASF B1253 source : AC4 21) chain B residue 57 type sequence H description BINDING SITE FOR RESIDUE ASF B1253 source : AC4 22) chain B residue 58 type sequence P description BINDING SITE FOR RESIDUE ASF B1253 source : AC4 23) chain B residue 59 type sequence K description BINDING SITE FOR RESIDUE ASF B1253 source : AC4 24) chain B residue 197 type sequence K description BINDING SITE FOR RESIDUE ASF B1253 source : AC4 25) chain B residue 95 type ACT_SITE sequence H description Electrophile source Swiss-Prot : SWS_FT_FI1 26) chain B residue 167 type ACT_SITE sequence E description Proton acceptor source Swiss-Prot : SWS_FT_FI2 27) chain B residue 11 type BINDING sequence N description source Swiss-Prot : SWS_FT_FI3 28) chain B residue 13 type BINDING sequence K description source Swiss-Prot : SWS_FT_FI3

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