eF-site ID 2vek-AB PDB Code 2vek Chain A, B click to enlarge Title Structure-based enzyme engineering efforts with an inactive monomeric TIM variant: the importance of a single point mutation for generating an active site with suitable binding properties Classification ISOMERASE Compound TRIOSEPHOSPHATE ISOMERASE Source null (TPIS_TRYBB) Sequence A:  SKPQPIAAANWKSGSPSLSELIDLFNSTSINHDVQCVVAS TFVHLAMTKERLSHPKFVIAAQNAGNADALASLKDFGVNW IVLGHSERRWYYGETNEIVADKVAAAVASGFMVIACIGET LQERESGRTAVVVLTQIAAIAKKLKKADWAKVVIAYEPVW AIGTGKVATPQQAQEAHALIRSWVSSKIGADVAGELRILY GGSVNGKNARTLYQQRDVNGFLAGLKPEFVDIIKATQ B:  SKPQPIAAANWKSGSPDSLSELIDLFNSTSINHDVQCVVA STFVHLAMTKERLSHPKFVIAAQNAGNADALASLKDFGVN WIVLGHSERRWYYGETNEIVADKVAAAVASGFMVIACIGE TLQERESGRTAVVVLTQIAAIAKKLKKADWAKVVIAYEPV WAIGTGKVATPQQAQEAHALIRSWVSSKIGADVAGELRIL YGGSVNGKNARTLYQQRDVNGFLAGLKPEFVDIIKATQ Description Functional site 1) chain A residue 11 type sequence N description BINDING SITE FOR RESIDUE CIT A1251 source : AC1 2) chain A residue 13 type sequence K description BINDING SITE FOR RESIDUE CIT A1251 source : AC1 3) chain A residue 95 type sequence H description BINDING SITE FOR RESIDUE CIT A1251 source : AC1 4) chain A residue 167 type sequence E description BINDING SITE FOR RESIDUE CIT A1251 source : AC1 5) chain A residue 171 type sequence A description BINDING SITE FOR RESIDUE CIT A1251 source : AC1 6) chain A residue 172 type sequence I description BINDING SITE FOR RESIDUE CIT A1251 source : AC1 7) chain A residue 173 type sequence G description BINDING SITE FOR RESIDUE CIT A1251 source : AC1 8) chain A residue 212 type sequence G description BINDING SITE FOR RESIDUE CIT A1251 source : AC1 9) chain A residue 213 type sequence S description BINDING SITE FOR RESIDUE CIT A1251 source : AC1 10) chain A residue 233 type sequence A description BINDING SITE FOR RESIDUE CIT A1251 source : AC1 11) chain A residue 234 type sequence G description BINDING SITE FOR RESIDUE CIT A1251 source : AC1 12) chain B residue 11 type sequence N description BINDING SITE FOR RESIDUE CIT B1251 source : AC2 13) chain B residue 13 type sequence K description BINDING SITE FOR RESIDUE CIT B1251 source : AC2 14) chain B residue 95 type sequence H description BINDING SITE FOR RESIDUE CIT B1251 source : AC2 15) chain B residue 167 type sequence E description BINDING SITE FOR RESIDUE CIT B1251 source : AC2 16) chain B residue 171 type sequence A description BINDING SITE FOR RESIDUE CIT B1251 source : AC2 17) chain B residue 172 type sequence I description BINDING SITE FOR RESIDUE CIT B1251 source : AC2 18) chain B residue 173 type sequence G description BINDING SITE FOR RESIDUE CIT B1251 source : AC2 19) chain B residue 213 type sequence S description BINDING SITE FOR RESIDUE CIT B1251 source : AC2 20) chain B residue 233 type sequence A description BINDING SITE FOR RESIDUE CIT B1251 source : AC2 21) chain B residue 234 type sequence G description BINDING SITE FOR RESIDUE CIT B1251 source : AC2 22) chain B residue 239 type sequence K description BINDING SITE FOR RESIDUE CIT B1251 source : AC2 23) chain B residue 29 type sequence N description BINDING SITE FOR RESIDUE TBU B1252 source : AC3 24) chain B residue 30 type sequence S description BINDING SITE FOR RESIDUE TBU B1252 source : AC3 25) chain B residue 31 type sequence T description BINDING SITE FOR RESIDUE TBU B1252 source : AC3 26) chain B residue 32 type sequence S description BINDING SITE FOR RESIDUE TBU B1252 source : AC3 27) chain B residue 193 type sequence W description BINDING SITE FOR RESIDUE TBU B1252 source : AC3 28) chain B residue 197 type sequence K description BINDING SITE FOR RESIDUE TBU B1252 source : AC3 29) chain A residue 26 type sequence D description BINDING SITE FOR RESIDUE ASF B1253 source : AC4 30) chain A residue 29 type sequence N description BINDING SITE FOR RESIDUE ASF B1253 source : AC4 31) chain A residue 30 type sequence S description BINDING SITE FOR RESIDUE ASF B1253 source : AC4 32) chain B residue 31 type sequence T description BINDING SITE FOR RESIDUE ASF B1253 source : AC4 33) chain B residue 32 type sequence S description BINDING SITE FOR RESIDUE ASF B1253 source : AC4 34) chain B residue 33 type sequence I description BINDING SITE FOR RESIDUE ASF B1253 source : AC4 35) chain B residue 57 type sequence H description BINDING SITE FOR RESIDUE ASF B1253 source : AC4 36) chain B residue 58 type sequence P description BINDING SITE FOR RESIDUE ASF B1253 source : AC4 37) chain B residue 59 type sequence K description BINDING SITE FOR RESIDUE ASF B1253 source : AC4 38) chain B residue 197 type sequence K description BINDING SITE FOR RESIDUE ASF B1253 source : AC4 39) chain A residue 165-175 type prosite sequence AYEPVWAIGTG description TIM_1 Triosephosphate isomerase active site. AYEPVWAIGTG source prosite : PS00171 40) chain B residue 95 type ACT_SITE sequence H description Electrophile source Swiss-Prot : SWS_FT_FI1 41) chain A residue 103 type ACT_SITE sequence G description Electrophile source Swiss-Prot : SWS_FT_FI1 42) chain B residue 167 type ACT_SITE sequence E description Proton acceptor source Swiss-Prot : SWS_FT_FI2 43) chain A residue 175 type ACT_SITE sequence G description Proton acceptor source Swiss-Prot : SWS_FT_FI2 44) chain B residue 11 type BINDING sequence N description source Swiss-Prot : SWS_FT_FI3 45) chain B residue 13 type BINDING sequence K description source Swiss-Prot : SWS_FT_FI3 46) chain A residue 11 type BINDING sequence N description source Swiss-Prot : SWS_FT_FI3 47) chain A residue 13 type BINDING sequence K description source Swiss-Prot : SWS_FT_FI3

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