eF-site ID 2vei-C PDB Code 2vei Chain C click to enlarge Title Structure-based enzyme engineering efforts with an inactive monomeric TIM variant: the importance of a single point mutation for generating an active site with suitable binding properties Classification ISOMERASE Compound GLYCOSOMAL TRIOSEPHOSPHATE ISOMERASE Source null (TPIS_TRYBB) Sequence C:  SKPQPIAAANWKSGSPDSLSELIDLFNSTSINHDVQCVVA STFVHLAMTKERLSHPKFVIAAQNAGNADALASLKDFGVN WIVLGHSERRWYYGETNEIVADKVAAAVASGFMVIACIGE TLQERESGRTAVVVLTQIAAIAKKLKKADWAKVVIAYEPV WAIGTGKVATPQQAQEAHALIRSWVSSKIGADVAGELRIL YGGSVNGKNARTLYQQRDVNGFLVGLKPEFVDIIKATQ Description Functional site 1) chain C residue 15 type sequence S description BINDING SITE FOR RESIDUE SO4 A1251 source : AC1 2) chain C residue 16 type sequence G description BINDING SITE FOR RESIDUE SO4 A1251 source : AC1 3) chain C residue 52 type sequence K description BINDING SITE FOR RESIDUE SO4 C1251 source : AC2 4) chain C residue 61 type sequence V description BINDING SITE FOR RESIDUE SO4 C1251 source : AC2 5) chain C residue 62 type sequence I description BINDING SITE FOR RESIDUE SO4 C1251 source : AC2 6) chain C residue 89 type sequence N description BINDING SITE FOR RESIDUE SO4 C1251 source : AC2 7) chain C residue 16 type sequence G description BINDING SITE FOR RESIDUE SO4 A1252 source : AC3 8) chain C residue 17 type sequence S description BINDING SITE FOR RESIDUE SO4 A1252 source : AC3 9) chain C residue 161 type sequence K description BINDING SITE FOR RESIDUE SO4 C1252 source : AC7 10) chain C residue 105 type sequence T description BINDING SITE FOR RESIDUE SO4 C1253 source : AC8 11) chain C residue 106 type sequence N description BINDING SITE FOR RESIDUE SO4 C1253 source : AC8 12) chain C residue 107 type sequence E description BINDING SITE FOR RESIDUE SO4 C1253 source : AC8 13) chain C residue 2 type sequence S description BINDING SITE FOR RESIDUE SO4 C1254 source : AC9 14) chain C residue 3 type sequence K description BINDING SITE FOR RESIDUE SO4 C1254 source : AC9 15) chain C residue 134 type sequence R description BINDING SITE FOR RESIDUE SO4 C1255 source : BC1 16) chain C residue 182 type sequence Q description BINDING SITE FOR RESIDUE SO4 C1255 source : BC1 17) chain C residue 103 type ACT_SITE sequence G description Electrophile source Swiss-Prot : SWS_FT_FI1 18) chain C residue 175 type ACT_SITE sequence G description Proton acceptor source Swiss-Prot : SWS_FT_FI2 19) chain C residue 11 type BINDING sequence N description source Swiss-Prot : SWS_FT_FI3 20) chain C residue 13 type BINDING sequence K description source Swiss-Prot : SWS_FT_FI3

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