eF-site ID 2vei-B PDB Code 2vei Chain B click to enlarge Title Structure-based enzyme engineering efforts with an inactive monomeric TIM variant: the importance of a single point mutation for generating an active site with suitable binding properties Classification ISOMERASE Compound GLYCOSOMAL TRIOSEPHOSPHATE ISOMERASE Source Trypanosoma brucei brucei (TPIS_TRYBB) Sequence B:  SKPQPIAAANWKSGSPDSLSELIDLFNSTSINHDVQCVVA STFVHLAMTKERLSHPKFVIAAQNAGNADALASLKDFGVN WIVLGHSERRWYYGETNEIVADKVAAAVASGFMVIACIGE TLQERESGRTAVVVLTQIAAIAKKLKKADWAKVVIAYEPV WAIGTGKVATPQQAQEAHALIRSWVSSKIGADVAGELRIL YGGSVNGKNARTLYQQRDVNGFLVGLKPEFVDIIKATQ Description Functional site 1) chain B residue 15 type sequence S description BINDING SITE FOR RESIDUE SO4 A1251 source : AC1 2) chain B residue 16 type sequence G description BINDING SITE FOR RESIDUE SO4 A1251 source : AC1 3) chain B residue 16 type sequence G description BINDING SITE FOR RESIDUE SO4 A1252 source : AC3 4) chain B residue 17 type sequence S description BINDING SITE FOR RESIDUE SO4 A1252 source : AC3 5) chain B residue 105 type sequence T description BINDING SITE FOR RESIDUE SO4 B1251 source : AC6 6) chain B residue 106 type sequence N description BINDING SITE FOR RESIDUE SO4 B1251 source : AC6 7) chain B residue 107 type sequence E description BINDING SITE FOR RESIDUE SO4 B1251 source : AC6 8) chain B residue 2 type sequence S description BINDING SITE FOR RESIDUE SO4 B1252 source : BC2 9) chain B residue 3 type sequence K description BINDING SITE FOR RESIDUE SO4 B1252 source : BC2 10) chain B residue 134 type sequence R description BINDING SITE FOR RESIDUE SO4 B1253 source : BC3 11) chain B residue 182 type sequence Q description BINDING SITE FOR RESIDUE SO4 B1253 source : BC3 12) chain B residue 13 type sequence K description BINDING SITE FOR RESIDUE SO4 B1254 source : BC5 13) chain B residue 213 type sequence S description BINDING SITE FOR RESIDUE SO4 B1254 source : BC5 14) chain B residue 234 type sequence G description BINDING SITE FOR RESIDUE SO4 B1254 source : BC5 15) chain B residue 239 type sequence K description BINDING SITE FOR RESIDUE SO4 B1254 source : BC5 16) chain B residue 44 type sequence T description BINDING SITE FOR RESIDUE SO4 B1255 source : BC6 17) chain B residue 45 type sequence F description BINDING SITE FOR RESIDUE SO4 B1255 source : BC6 18) chain B residue 65 type sequence Q description BINDING SITE FOR RESIDUE SO4 B1255 source : BC6 19) chain B residue 66 type sequence N description BINDING SITE FOR RESIDUE SO4 B1255 source : BC6 20) chain B residue 11 type BINDING sequence N description source Swiss-Prot : SWS_FT_FI3 21) chain B residue 13 type BINDING sequence K description source Swiss-Prot : SWS_FT_FI3 22) chain B residue 95 type ACT_SITE sequence H description Electrophile source Swiss-Prot : SWS_FT_FI1 23) chain B residue 167 type ACT_SITE sequence E description Proton acceptor source Swiss-Prot : SWS_FT_FI2

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