eF-site ID 2o7m-AB PDB Code 2o7m Chain A, B click to enlarge Title The C-terminal loop of the homing endonuclease I-CreI is essential for DNA binding and cleavage. Identification of a novel site for specificity engineering in the I-CreI scaffold Classification HYDROLASE Compound DNA endonuclease I-CreI Source Chlamydomonas reinhardtii (Chlamydomonas smithii) (DNE1_CHLRE) Sequence A:  NTKYNKEFLLYLAGFVDGDGSIIAQIKPNQSYKFKHQLSL TFQVTQKTQRRWFLDKLVDEIGVGYVRDRGSVSDYILSEI KPLHNFLTQLQPFLKLKQKQANLVLKIIEQLPSAKESPDK FLEVCTWVDQIAALNDSKTRKTTSETVRAVLDS B:  NTKYNKEFLLYLAGFVDGDGSIIAQIKPNQSYKFKHQLSL TFQVTQKTQRRWFLDKLVDEIGVGYVRDRGSVSDYILSEI KPLHNFLTQLQPFLKLKQKQANLVLKIIEQLPSAKESPDK FLEVCTWVDQIAALNDSKTRKTTSETVRAVLDS Description Functional site 1) chain A residue 19 type catalytic sequence G description 233 source MCSA : MCSA1 2) chain A residue 20 type catalytic sequence D description 233 source MCSA : MCSA1 3) chain B residue 19 type catalytic sequence G description 233 source MCSA : MCSA2 4) chain B residue 20 type catalytic sequence D description 233 source MCSA : MCSA2 5) chain A residue 19 type BINDING sequence G description source Swiss-Prot : SWS_FT_FI1 6) chain A residue 20 type BINDING sequence D description source Swiss-Prot : SWS_FT_FI1 7) chain B residue 19 type BINDING sequence G description source Swiss-Prot : SWS_FT_FI1 8) chain B residue 20 type BINDING sequence D description source Swiss-Prot : SWS_FT_FI1

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