eF-site ID 2o7m-A
PDB Code 2o7m
Chain A

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Title The C-terminal loop of the homing endonuclease I-CreI is essential for DNA binding and cleavage. Identification of a novel site for specificity engineering in the I-CreI scaffold
Classification HYDROLASE
Compound DNA endonuclease I-CreI
Source Chlamydomonas reinhardtii (Chlamydomonas smithii) (DNE1_CHLRE)
Sequence A:  NTKYNKEFLLYLAGFVDGDGSIIAQIKPNQSYKFKHQLSL
TFQVTQKTQRRWFLDKLVDEIGVGYVRDRGSVSDYILSEI
KPLHNFLTQLQPFLKLKQKQANLVLKIIEQLPSAKESPDK
FLEVCTWVDQIAALNDSKTRKTTSETVRAVLDS
Description


Functional site

1) chain A
residue 19
type catalytic
sequence G
description 233
source MCSA : MCSA1

2) chain A
residue 20
type catalytic
sequence D
description 233
source MCSA : MCSA1

3) chain A
residue 19
type BINDING
sequence G
description
source Swiss-Prot : SWS_FT_FI1

4) chain A
residue 20
type BINDING
sequence D
description
source Swiss-Prot : SWS_FT_FI1


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