|
eF-site ID
|
2o7m-A |
PDB Code
|
2o7m |
Chain
|
A |
|
click to enlarge
|
|
Title
|
The C-terminal loop of the homing endonuclease I-CreI is essential for DNA binding and cleavage. Identification of a novel site for specificity engineering in the I-CreI scaffold |
Classification
|
HYDROLASE |
Compound
|
DNA endonuclease I-CreI |
Source
|
Chlamydomonas reinhardtii (Chlamydomonas smithii) (DNE1_CHLRE) |
|
Sequence
|
A: |
NTKYNKEFLLYLAGFVDGDGSIIAQIKPNQSYKFKHQLSL
TFQVTQKTQRRWFLDKLVDEIGVGYVRDRGSVSDYILSEI
KPLHNFLTQLQPFLKLKQKQANLVLKIIEQLPSAKESPDK
FLEVCTWVDQIAALNDSKTRKTTSETVRAVLDS
|
|
Description
|
|
Functional site
|
|
1)
|
chain |
A |
residue |
19 |
type |
catalytic |
sequence |
G
|
description |
233
|
source |
MCSA : MCSA1
|
|
2)
|
chain |
A |
residue |
20 |
type |
catalytic |
sequence |
D
|
description |
233
|
source |
MCSA : MCSA1
|
|
3)
|
chain |
A |
residue |
19 |
type |
BINDING |
sequence |
G
|
description |
|
source |
Swiss-Prot : SWS_FT_FI1
|
|
4)
|
chain |
A |
residue |
20 |
type |
BINDING |
sequence |
D
|
description |
|
source |
Swiss-Prot : SWS_FT_FI1
|
|
|
|