eF-site ID 2ewf-A PDB Code 2ewf Chain A click to enlarge Title Crystal structure of the site-specific DNA nickase N.BspD6I Classification HYDROLASE Compound Nicking endonuclease N.BspD6I Source Bacillus sp (Q8GCA3_BACSP) Sequence A:  MAKKVNWYVSCSPRSPEKIQPELKVLANFEGSYWKGVKGY KAQEAFAKELAALPQFLGAFSTRDRVAPMKTYGFVFVDEE GYLRITEAGKMLANNRRPKDVFLKQLVKWQYPSFQHKGKE YPEEEWSINPLVFVLSLLKKVGGLSKLDIAMFCLTATNNN QVDEIAEEIMQFRNEREKIKGQNKKLEFTENYFFKRFEKI YGNVSHKSKIETKMRNARDVADATTRYFRYTGLFVARGNQ LVLNPEKSDLIDEIISSSKVVKNYTRVEEFHEYYGNPSLP QFSFETKEQLLDLAHRIRDENTRLAEQLVEHFPNVKVEIQ VLEDIYNSLNKKVDVETLKDVIYHAKELQLELKKKKLQAD FNDPRQLEEVIDLLEVYHEKKNVIEEKIKARFIANKNTVF EWLTWNGFIILGNALEYKNNFVIDEELQPVTHAAGNQPDM EIIYEDFIVLGEVTTSKGATQFKMESEPVTRHYLNKKKEL EKQGVEKELYCLFIAPEINKNTFEEFMKYNIVQNTRIIPL SLKQFNMLLMVQKKLIEKGRRLSSYDIKNLMVSLYRTTIE CERKYTQIKAGLEETLNNWVVDKEVRF Description Functional site 1) chain A residue 190 type sequence N description BINDING SITE FOR RESIDUE BR A 605 source : AC1 2) chain A residue 191 type sequence K description BINDING SITE FOR RESIDUE BR A 605 source : AC1 3) chain A residue 286 type sequence E description BINDING SITE FOR RESIDUE BR A 605 source : AC1 4) chain A residue 54 type sequence P description BINDING SITE FOR RESIDUE BR A 606 source : AC2 5) chain A residue 55 type sequence Q description BINDING SITE FOR RESIDUE BR A 606 source : AC2 6) chain A residue 75 type sequence P description BINDING SITE FOR RESIDUE BR A 607 source : AC3 7) chain A residue 78 type sequence T description BINDING SITE FOR RESIDUE BR A 607 source : AC3 8) chain A residue 413 type sequence K description BINDING SITE FOR RESIDUE BR A 608 source : AC4 9) chain A residue 243 type sequence R description BINDING SITE FOR RESIDUE BR A 609 source : AC5 10) chain A residue 415 type sequence T description BINDING SITE FOR RESIDUE BR A 609 source : AC5 11) chain A residue 449 type sequence H description BINDING SITE FOR RESIDUE BR A 609 source : AC5 12) chain A residue 84 type sequence V description BINDING SITE FOR RESIDUE BR A 610 source : AC6 13) chain A residue 122 type sequence Q description BINDING SITE FOR RESIDUE BR A 610 source : AC6 14) chain A residue 401 type sequence I description BINDING SITE FOR RESIDUE BR A 611 source : AC7 15) chain A residue 413 type sequence K description BINDING SITE FOR RESIDUE BR A 611 source : AC7 16) chain A residue 34 type sequence W description BINDING SITE FOR RESIDUE BR A 612 source : AC8 17) chain A residue 330 type sequence P description BINDING SITE FOR RESIDUE BR A 613 source : AC9 18) chain A residue 331 type sequence N description BINDING SITE FOR RESIDUE BR A 613 source : AC9 19) chain A residue 247 type sequence Y description BINDING SITE FOR RESIDUE BR A 614 source : BC1 20) chain A residue 440 type sequence I description BINDING SITE FOR RESIDUE BR A 614 source : BC1 21) chain A residue 183 type sequence R description BINDING SITE FOR RESIDUE BR A 617 source : BC2 22) chain A residue 184 type sequence E description BINDING SITE FOR RESIDUE BR A 617 source : BC2

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