eF-site ID 1zzt-AB PDB Code 1zzt Chain A, B click to enlarge Title Bovine eNOS N368D/V106M double mutant with L-N(omega)-Nitroarginine-(4R)-Amino-L-Proline Amide Bound Classification OXIDOREDUCTASE Compound Nitric-oxide synthase, endothelial Source Bos taurus (Bovine) (NOS3_BOVIN) Sequence A:  GPKFPRVKNWELGSITYDTLCAQSQQDGPCTPRRCLGSLM LPPPAEQLLSQARDFINQYYSSIKRSGSQAHEERLQEVEA EVASTGTYHLRESELVFGAKQAWRNAPRCVGRIQWGKLQV FDARDCSSAQEMFTYICNHIKYATNRGNLRSAITVFPQRA PGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCI QHGWTPGNGRFDVLPLLLQAPDEAPELFVLPPELVLEVPL EHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFSAAPFSG WYMSTEIGTRDLCDPHRYNILEDVAVXMDLDTRTTSSLWK DKAAVEINLAVLHSFQLAKVTIVDHHAATVSFMKHLDNEQ KARGGCPADWAWIVPPISGSLTPVFHQEMVNYILSPAFRY QPDPW B:  KFPRVKNWELGSITYDTLCAQSQQDGPCTPRRCLGSLMLP RPPPAEQLLSQARDFINQYYSSIKRSGSQAHEERLQEVEA EVASTGTYHLRESELVFGAKQAWRNAPRCVGRIQWGKLQV FDARDCSSAQEMFTYICNHIKYATNRGNLRSAITVFPQRA PGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCI QHGWTPGNGRFDVLPLLLQAPDEAPELFVLPPELVLEVPL EHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFSAAPFSG WYMSTEIGTRDLCDPHRYNILEDVAVXMDLDTRTTSSLWK DKAAVEINLAVLHSFQLAKVTIVDHHAATVSFMKHLDNEQ KARGGCPADWAWIVPPISGSLTPVFHQEMVNYILSPAFRY QPDPW Description Functional site 1) chain A residue 188 type sequence G description BINDING SITE FOR RESIDUE ACT A 860 source : AC1 2) chain A residue 358 type sequence W description BINDING SITE FOR RESIDUE ACT A 860 source : AC1 3) chain A residue 428 type sequence S description BINDING SITE FOR RESIDUE ACT A 860 source : AC1 4) chain B residue 358 type sequence W description BINDING SITE FOR RESIDUE ACT B 861 source : AC2 5) chain B residue 420 type sequence V description BINDING SITE FOR RESIDUE ACT B 861 source : AC2 6) chain B residue 428 type sequence S description BINDING SITE FOR RESIDUE ACT B 861 source : AC2 7) chain A residue 96 type sequence C description BINDING SITE FOR RESIDUE ZN A 900 source : AC3 8) chain A residue 101 type sequence C description BINDING SITE FOR RESIDUE ZN A 900 source : AC3 9) chain B residue 96 type sequence C description BINDING SITE FOR RESIDUE ZN A 900 source : AC3 10) chain B residue 101 type sequence C description BINDING SITE FOR RESIDUE ZN A 900 source : AC3 11) chain A residue 180 type sequence W description BINDING SITE FOR RESIDUE HEM A 700 source : AC4 12) chain A residue 185 type sequence R description BINDING SITE FOR RESIDUE HEM A 700 source : AC4 13) chain A residue 186 type sequence C description BINDING SITE FOR RESIDUE HEM A 700 source : AC4 14) chain A residue 187 type sequence V description BINDING SITE FOR RESIDUE HEM A 700 source : AC4 15) chain A residue 355 type sequence F description BINDING SITE FOR RESIDUE HEM A 700 source : AC4 16) chain A residue 356 type sequence S description BINDING SITE FOR RESIDUE HEM A 700 source : AC4 17) chain A residue 358 type sequence W description BINDING SITE FOR RESIDUE HEM A 700 source : AC4 18) chain A residue 363 type sequence E description BINDING SITE FOR RESIDUE HEM A 700 source : AC4 19) chain A residue 449 type sequence W description BINDING SITE FOR RESIDUE HEM A 700 source : AC4 20) chain A residue 475 type sequence F description BINDING SITE FOR RESIDUE HEM A 700 source : AC4 21) chain A residue 477 type sequence Y description BINDING SITE FOR RESIDUE HEM A 700 source : AC4 22) chain A residue 104 type sequence S description BINDING SITE FOR RESIDUE H4B A 760 source : AC5 23) chain A residue 106 type sequence M description BINDING SITE FOR RESIDUE H4B A 760 source : AC5 24) chain A residue 367 type sequence R description BINDING SITE FOR RESIDUE H4B A 760 source : AC5 25) chain A residue 448 type sequence A description BINDING SITE FOR RESIDUE H4B A 760 source : AC5 26) chain A residue 449 type sequence W description BINDING SITE FOR RESIDUE H4B A 760 source : AC5 27) chain B residue 447 type sequence W description BINDING SITE FOR RESIDUE H4B A 760 source : AC5 28) chain B residue 462 type sequence F description BINDING SITE FOR RESIDUE H4B A 760 source : AC5 29) chain B residue 463 type sequence H description BINDING SITE FOR RESIDUE H4B A 760 source : AC5 30) chain B residue 464 type sequence Q description BINDING SITE FOR RESIDUE H4B A 760 source : AC5 31) chain B residue 465 type sequence E description BINDING SITE FOR RESIDUE H4B A 760 source : AC5 32) chain A residue 249 type sequence Q description BINDING SITE FOR RESIDUE DP9 A 799 source : AC6 33) chain A residue 336 type sequence P description BINDING SITE FOR RESIDUE DP9 A 799 source : AC6 34) chain A residue 338 type sequence V description BINDING SITE FOR RESIDUE DP9 A 799 source : AC6 35) chain A residue 355 type sequence F description BINDING SITE FOR RESIDUE DP9 A 799 source : AC6 36) chain A residue 356 type sequence S description BINDING SITE FOR RESIDUE DP9 A 799 source : AC6 37) chain A residue 357 type sequence G description BINDING SITE FOR RESIDUE DP9 A 799 source : AC6 38) chain A residue 358 type sequence W description BINDING SITE FOR RESIDUE DP9 A 799 source : AC6 39) chain A residue 363 type sequence E description BINDING SITE FOR RESIDUE DP9 A 799 source : AC6 40) chain A residue 477 type sequence Y description BINDING SITE FOR RESIDUE DP9 A 799 source : AC6 41) chain B residue 180 type sequence W description BINDING SITE FOR RESIDUE HEM B 700 source : AC7 42) chain B residue 185 type sequence R description BINDING SITE FOR RESIDUE HEM B 700 source : AC7 43) chain B residue 186 type sequence C description BINDING SITE FOR RESIDUE HEM B 700 source : AC7 44) chain B residue 187 type sequence V description BINDING SITE FOR RESIDUE HEM B 700 source : AC7 45) chain B residue 228 type sequence S description BINDING SITE FOR RESIDUE HEM B 700 source : AC7 46) chain B residue 355 type sequence F description BINDING SITE FOR RESIDUE HEM B 700 source : AC7 47) chain B residue 356 type sequence S description BINDING SITE FOR RESIDUE HEM B 700 source : AC7 48) chain B residue 357 type sequence G description BINDING SITE FOR RESIDUE HEM B 700 source : AC7 49) chain B residue 358 type sequence W description BINDING SITE FOR RESIDUE HEM B 700 source : AC7 50) chain B residue 363 type sequence E description BINDING SITE FOR RESIDUE HEM B 700 source : AC7 51) chain B residue 449 type sequence W description BINDING SITE FOR RESIDUE HEM B 700 source : AC7 52) chain B residue 475 type sequence F description BINDING SITE FOR RESIDUE HEM B 700 source : AC7 53) chain B residue 477 type sequence Y description BINDING SITE FOR RESIDUE HEM B 700 source : AC7 54) chain A residue 447 type sequence W description BINDING SITE FOR RESIDUE H4B B 761 source : AC8 55) chain A residue 462 type sequence F description BINDING SITE FOR RESIDUE H4B B 761 source : AC8 56) chain A residue 463 type sequence H description BINDING SITE FOR RESIDUE H4B B 761 source : AC8 57) chain A residue 464 type sequence Q description BINDING SITE FOR RESIDUE H4B B 761 source : AC8 58) chain A residue 465 type sequence E description BINDING SITE FOR RESIDUE H4B B 761 source : AC8 59) chain B residue 104 type sequence S description BINDING SITE FOR RESIDUE H4B B 761 source : AC8 60) chain B residue 106 type sequence M description BINDING SITE FOR RESIDUE H4B B 761 source : AC8 61) chain B residue 367 type sequence R description BINDING SITE FOR RESIDUE H4B B 761 source : AC8 62) chain B residue 448 type sequence A description BINDING SITE FOR RESIDUE H4B B 761 source : AC8 63) chain B residue 449 type sequence W description BINDING SITE FOR RESIDUE H4B B 761 source : AC8 64) chain B residue 249 type sequence Q description BINDING SITE FOR RESIDUE DP9 B 800 source : AC9 65) chain B residue 336 type sequence P description BINDING SITE FOR RESIDUE DP9 B 800 source : AC9 66) chain B residue 338 type sequence V description BINDING SITE FOR RESIDUE DP9 B 800 source : AC9 67) chain B residue 356 type sequence S description BINDING SITE FOR RESIDUE DP9 B 800 source : AC9 68) chain B residue 357 type sequence G description BINDING SITE FOR RESIDUE DP9 B 800 source : AC9 69) chain B residue 358 type sequence W description BINDING SITE FOR RESIDUE DP9 B 800 source : AC9 70) chain B residue 363 type sequence E description BINDING SITE FOR RESIDUE DP9 B 800 source : AC9 71) chain B residue 477 type sequence Y description BINDING SITE FOR RESIDUE DP9 B 800 source : AC9 72) chain A residue 97 type BINDING sequence T description BINDING => ECO:0000250|UniProtKB:P35228 source Swiss-Prot : SWS_FT_FI1 73) chain A residue 102 type BINDING sequence L description BINDING => ECO:0000250|UniProtKB:P35228 source Swiss-Prot : SWS_FT_FI1 74) chain B residue 97 type BINDING sequence T description BINDING => ECO:0000250|UniProtKB:P35228 source Swiss-Prot : SWS_FT_FI1 75) chain B residue 102 type BINDING sequence L description BINDING => ECO:0000250|UniProtKB:P35228 source Swiss-Prot : SWS_FT_FI1 76) chain A residue 105 type BINDING sequence L description BINDING => ECO:0000250|UniProtKB:P29474 source Swiss-Prot : SWS_FT_FI2 77) chain A residue 478 type BINDING sequence Q description BINDING => ECO:0000250|UniProtKB:P29474 source Swiss-Prot : SWS_FT_FI2 78) chain B residue 105 type BINDING sequence L description BINDING => ECO:0000250|UniProtKB:P29474 source Swiss-Prot : SWS_FT_FI2 79) chain B residue 250 type BINDING sequence L description BINDING => ECO:0000250|UniProtKB:P29474 source Swiss-Prot : SWS_FT_FI2 80) chain B residue 359 type BINDING sequence Y description BINDING => ECO:0000250|UniProtKB:P29474 source Swiss-Prot : SWS_FT_FI2 81) chain B residue 360 type BINDING sequence M description BINDING => ECO:0000250|UniProtKB:P29474 source Swiss-Prot : SWS_FT_FI2 82) chain B residue 364 type BINDING sequence I description BINDING => ECO:0000250|UniProtKB:P29474 source Swiss-Prot : SWS_FT_FI2 83) chain B residue 369 type BINDING sequence L description BINDING => ECO:0000250|UniProtKB:P29474 source Swiss-Prot : SWS_FT_FI2 84) chain B residue 449 type BINDING sequence W description BINDING => ECO:0000250|UniProtKB:P29474 source Swiss-Prot : SWS_FT_FI2 85) chain B residue 450 type BINDING sequence I description BINDING => ECO:0000250|UniProtKB:P29474 source Swiss-Prot : SWS_FT_FI2 86) chain B residue 463 type BINDING sequence H description BINDING => ECO:0000250|UniProtKB:P29474 source Swiss-Prot : SWS_FT_FI2 87) chain A residue 250 type BINDING sequence L description BINDING => ECO:0000250|UniProtKB:P29474 source Swiss-Prot : SWS_FT_FI2 88) chain B residue 478 type BINDING sequence Q description BINDING => ECO:0000250|UniProtKB:P29474 source Swiss-Prot : SWS_FT_FI2 89) chain A residue 359 type BINDING sequence Y description BINDING => ECO:0000250|UniProtKB:P29474 source Swiss-Prot : SWS_FT_FI2 90) chain A residue 360 type BINDING sequence M description BINDING => ECO:0000250|UniProtKB:P29474 source Swiss-Prot : SWS_FT_FI2 91) chain A residue 364 type BINDING sequence I description BINDING => ECO:0000250|UniProtKB:P29474 source Swiss-Prot : SWS_FT_FI2 92) chain A residue 369 type BINDING sequence L description BINDING => ECO:0000250|UniProtKB:P29474 source Swiss-Prot : SWS_FT_FI2 93) chain A residue 449 type BINDING sequence W description BINDING => ECO:0000250|UniProtKB:P29474 source Swiss-Prot : SWS_FT_FI2 94) chain A residue 450 type BINDING sequence I description BINDING => ECO:0000250|UniProtKB:P29474 source Swiss-Prot : SWS_FT_FI2 95) chain A residue 463 type BINDING sequence H description BINDING => ECO:0000250|UniProtKB:P29474 source Swiss-Prot : SWS_FT_FI2 96) chain A residue 187 type BINDING sequence V description axial binding residue => ECO:0000250|UniProtKB:P29474 source Swiss-Prot : SWS_FT_FI3 97) chain B residue 187 type BINDING sequence V description axial binding residue => ECO:0000250|UniProtKB:P29474 source Swiss-Prot : SWS_FT_FI3 98) chain A residue 185-192 type prosite sequence RCVGRIQW description NOS Nitric oxide synthase (NOS) signature. RCVGRIqW source prosite : PS60001

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