eF-site/Summary 1zcd-AB

eF-site ID	1zcd-AB
PDB Code	1zcd
Chain	A, B

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Title	Crystal structure of the Na+/H+ antiporter NhaA
Classification	MEMBRANE PROTEIN
Compound	Na(+)/H(+) antiporter 1
Source	Escherichia coli (strain K12) (NHAA_ECOLI)

Sequence	A:	SSDASGGIILIIAAILAMIMANSGATSGWYHDFLETPVQL RVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSL ASLRQAAFPVIAAIGGMIVPALLYLAFNYADPITREGWAI PAATDIAFALGVLALLGSRVPLALKIFLMALAIIDDLGAI IIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGARRTGVY ILVGVVLWTAVLKSGVHATLAGVIVGFFIPLKEKHGRSPA KRLEHVLHPWVAYLILPLFAFANAGVSLQGVTLDGLTSIL PLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQI MVVGILCGIGFTMSIFIASLAFGSVDPELINWAKLGILVG SISSAVIGYSWLRVRL
	B:	SSDASGGIILIIAAILAMIMANSGATSGWYHDFLETPVQL RVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSL ASLRQAAFPVIAAIGGMIVPALLYLAFNYADPITREGWAI PAATDIAFALGVLALLGSRVPLALKIFLMALAIIDDLGAI IIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGARRTGVY ILVGVVLWTAVLKSGVHATLAGVIVGFFIPLKEKHGRSPA KRLEHVLHPWVAYLILPLFAFANAGVSLQGVTLDGLTSIL PLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQI MVVGILCGIGFTMSIFIASLAFGSVDPELINWAKLGILVG SISSAVIGYSWLRVRL

Description

Functional site


1)	chain	B
	residue	201-204
	type	TOPO_DOM
	sequence	GARR
	description	Cytoplasmic => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI1

2)	chain	B
	residue	237-253
	type	TOPO_DOM
	sequence	IPLKEKHGRSPAKRLEH
	description	Cytoplasmic => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI1

3)	chain	B
	residue	311-339
	type	TOPO_DOM
	sequence	ALRLKLAHLPEGTTYQQIMVVGILCGIGF
	description	Cytoplasmic => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI1

4)	chain	A
	residue	81-96
	type	TOPO_DOM
	sequence	RELMQGSLASLRQAAF
	description	Cytoplasmic => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI1

5)	chain	A
	residue	131-154
	type	TOPO_DOM
	sequence	ATDIAFALGVLALLGSRVPLALKI
	description	Cytoplasmic => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI1

6)	chain	A
	residue	201-204
	type	TOPO_DOM
	sequence	GARR
	description	Cytoplasmic => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI1

7)	chain	A
	residue	237-253
	type	TOPO_DOM
	sequence	IPLKEKHGRSPAKRLEH
	description	Cytoplasmic => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI1

8)	chain	A
	residue	311-339
	type	TOPO_DOM
	sequence	ALRLKLAHLPEGTTYQQIMVVGILCGIGF
	description	Cytoplasmic => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI1

9)	chain	B
	residue	81-96
	type	TOPO_DOM
	sequence	RELMQGSLASLRQAAF
	description	Cytoplasmic => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI1

10)	chain	B
	residue	131-154
	type	TOPO_DOM
	sequence	ATDIAFALGVLALLGSRVPLALKI
	description	Cytoplasmic => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI1

11)	chain	A
	residue	224-236
	type	TRANSMEM
	sequence	VHATLAGVIVGFF
	description	Helical; Name=8 => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI10

12)	chain	B
	residue	224-236
	type	TRANSMEM
	sequence	VHATLAGVIVGFF
	description	Helical; Name=8 => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI10

13)	chain	A
	residue	254-272
	type	TRANSMEM
	sequence	VLHPWVAYLILPLFAFANA
	description	Helical; Name=9 => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI11

14)	chain	B
	residue	254-272
	type	TRANSMEM
	sequence	VLHPWVAYLILPLFAFANA
	description	Helical; Name=9 => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI11

15)	chain	A
	residue	32-58
	type	TOPO_DOM
	sequence	GATSGWYHDFLETPVQLRVGSLEINKN
	description	Periplasmic => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI3

16)	chain	B
	residue	223
	type	TOPO_DOM
	sequence	G
	description	Periplasmic => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI3

17)	chain	B
	residue	273-286
	type	TOPO_DOM
	sequence	GVSLQGVTLDGLTS
	description	Periplasmic => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI3

18)	chain	B
	residue	351-357
	type	TOPO_DOM
	sequence	GSVDPEL
	description	Periplasmic => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI3

19)	chain	A
	residue	117-122
	type	TOPO_DOM
	sequence	YADPIT
	description	Periplasmic => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI3

20)	chain	A
	residue	177-180
	type	TOPO_DOM
	sequence	NDLS
	description	Periplasmic => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI3

21)	chain	A
	residue	223
	type	TOPO_DOM
	sequence	G
	description	Periplasmic => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI3

22)	chain	A
	residue	273-286
	type	TOPO_DOM
	sequence	GVSLQGVTLDGLTS
	description	Periplasmic => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI3

23)	chain	A
	residue	351-357
	type	TOPO_DOM
	sequence	GSVDPEL
	description	Periplasmic => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI3

24)	chain	B
	residue	32-58
	type	TOPO_DOM
	sequence	GATSGWYHDFLETPVQLRVGSLEINKN
	description	Periplasmic => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI3

25)	chain	B
	residue	117-122
	type	TOPO_DOM
	sequence	YADPIT
	description	Periplasmic => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI3

26)	chain	B
	residue	177-180
	type	TOPO_DOM
	sequence	NDLS
	description	Periplasmic => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI3

27)	chain	A
	residue	163
	type	SITE
	sequence	D
	description	Essential for cation binding and translocation => ECO:0000305\|PubMed:15988517, ECO:0000305\|PubMed:19274728, ECO:0000305\|PubMed:22915592, ECO:0000305\|PubMed:25422503, ECO:0000305\|PubMed:27021484, ECO:0000305\|PubMed:27708266, ECO:0000305\|PubMed:30409911, ECO:0000305\|PubMed:7737413
	source	Swiss-Prot : SWS_FT_FI17

28)	chain	B
	residue	163
	type	SITE
	sequence	D
	description	Essential for cation binding and translocation => ECO:0000305\|PubMed:15988517, ECO:0000305\|PubMed:19274728, ECO:0000305\|PubMed:22915592, ECO:0000305\|PubMed:25422503, ECO:0000305\|PubMed:27021484, ECO:0000305\|PubMed:27708266, ECO:0000305\|PubMed:30409911, ECO:0000305\|PubMed:7737413
	source	Swiss-Prot : SWS_FT_FI17

29)	chain	A
	residue	164
	type	SITE
	sequence	D
	description	Essential for cation binding and translocation => ECO:0000305\|PubMed:15988517, ECO:0000305\|PubMed:19274728, ECO:0000305\|PubMed:22915592, ECO:0000305\|PubMed:25422503, ECO:0000305\|PubMed:27021484, ECO:0000305\|PubMed:27708266, ECO:0000305\|PubMed:7737413
	source	Swiss-Prot : SWS_FT_FI18

30)	chain	B
	residue	164
	type	SITE
	sequence	D
	description	Essential for cation binding and translocation => ECO:0000305\|PubMed:15988517, ECO:0000305\|PubMed:19274728, ECO:0000305\|PubMed:22915592, ECO:0000305\|PubMed:25422503, ECO:0000305\|PubMed:27021484, ECO:0000305\|PubMed:27708266, ECO:0000305\|PubMed:7737413
	source	Swiss-Prot : SWS_FT_FI18

31)	chain	A
	residue	241
	type	SITE
	sequence	E
	description	Important for pH response => ECO:0000305\|PubMed:10455127
	source	Swiss-Prot : SWS_FT_FI19

32)	chain	A
	residue	254
	type	SITE
	sequence	V
	description	Important for pH response => ECO:0000305\|PubMed:10455127
	source	Swiss-Prot : SWS_FT_FI19

33)	chain	B
	residue	241
	type	SITE
	sequence	E
	description	Important for pH response => ECO:0000305\|PubMed:10455127
	source	Swiss-Prot : SWS_FT_FI19

34)	chain	B
	residue	254
	type	SITE
	sequence	V
	description	Important for pH response => ECO:0000305\|PubMed:10455127
	source	Swiss-Prot : SWS_FT_FI19

35)	chain	A
	residue	252
	type	SITE
	sequence	E
	description	Important for pH response => ECO:0000305\|PubMed:14604993
	source	Swiss-Prot : SWS_FT_FI20

36)	chain	B
	residue	252
	type	SITE
	sequence	E
	description	Important for pH response => ECO:0000305\|PubMed:14604993
	source	Swiss-Prot : SWS_FT_FI20

37)	chain	A
	residue	300
	type	SITE
	sequence	K
	description	Important for stability and activity => ECO:0000305\|PubMed:15988517, ECO:0000305\|PubMed:22915592, ECO:0000305\|PubMed:25422503, ECO:0000305\|PubMed:27708266, ECO:0000305\|PubMed:28330875, ECO:0000305\|PubMed:30409911
	source	Swiss-Prot : SWS_FT_FI21

38)	chain	B
	residue	300
	type	SITE
	sequence	K
	description	Important for stability and activity => ECO:0000305\|PubMed:15988517, ECO:0000305\|PubMed:22915592, ECO:0000305\|PubMed:25422503, ECO:0000305\|PubMed:27708266, ECO:0000305\|PubMed:28330875, ECO:0000305\|PubMed:30409911
	source	Swiss-Prot : SWS_FT_FI21

39)	chain	A
	residue	287-310
	type	TRANSMEM
	sequence	ILPLGIIAGLLIGKPLGISLFCWL
	description	Helical; Name=10 => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI12

40)	chain	B
	residue	287-310
	type	TRANSMEM
	sequence	ILPLGIIAGLLIGKPLGISLFCWL
	description	Helical; Name=10 => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI12

41)	chain	A
	residue	340-350
	type	TRANSMEM
	sequence	TMSIFIASLAF
	description	Helical; Name=11 => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI13

42)	chain	B
	residue	340-350
	type	TRANSMEM
	sequence	TMSIFIASLAF
	description	Helical; Name=11 => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI13

43)	chain	A
	residue	358-380
	type	TRANSMEM
	sequence	INWAKLGILVGSISSAVIGYSWL
	description	Helical; Name=12 => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI14

44)	chain	B
	residue	358-380
	type	TRANSMEM
	sequence	INWAKLGILVGSISSAVIGYSWL
	description	Helical; Name=12 => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI14

45)	chain	A
	residue	133
	type	SITE
	sequence	D
	description	Important for stability, cation binding and translocation => ECO:0000305\|PubMed:15988517, ECO:0000305\|PubMed:22915592, ECO:0000305\|PubMed:27021484, ECO:0000305\|PubMed:29410365, ECO:0000305\|PubMed:7737413
	source	Swiss-Prot : SWS_FT_FI16

46)	chain	B
	residue	133
	type	SITE
	sequence	D
	description	Important for stability, cation binding and translocation => ECO:0000305\|PubMed:15988517, ECO:0000305\|PubMed:22915592, ECO:0000305\|PubMed:27021484, ECO:0000305\|PubMed:29410365, ECO:0000305\|PubMed:7737413
	source	Swiss-Prot : SWS_FT_FI16

47)	chain	A
	residue	12-31
	type	TRANSMEM
	sequence	ASGGIILIIAAILAMIMANS
	description	Helical; Name=1 => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI2

48)	chain	B
	residue	12-31
	type	TRANSMEM
	sequence	ASGGIILIIAAILAMIMANS
	description	Helical; Name=1 => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI2

49)	chain	A
	residue	59-80
	type	TRANSMEM
	sequence	MLLWINDALMAVFFLLVGLEVK
	description	Helical; Name=2 => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI4

50)	chain	B
	residue	59-80
	type	TRANSMEM
	sequence	MLLWINDALMAVFFLLVGLEVK
	description	Helical; Name=2 => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI4

51)	chain	A
	residue	97-116
	type	TRANSMEM
	sequence	PVIAAIGGMIVPALLYLAFN
	description	Helical; Name=3 => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI5

52)	chain	B
	residue	97-116
	type	TRANSMEM
	sequence	PVIAAIGGMIVPALLYLAFN
	description	Helical; Name=3 => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI5

53)	chain	A
	residue	123-130
	type	TRANSMEM
	sequence	REGWAIPA
	description	Helical; Name=4 => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI6

54)	chain	B
	residue	123-130
	type	TRANSMEM
	sequence	REGWAIPA
	description	Helical; Name=4 => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI6

55)	chain	A
	residue	155-176
	type	TRANSMEM
	sequence	FLMALAIIDDLGAIIIIALFYT
	description	Helical; Name=5 => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI7

56)	chain	B
	residue	155-176
	type	TRANSMEM
	sequence	FLMALAIIDDLGAIIIIALFYT
	description	Helical; Name=5 => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI7

57)	chain	A
	residue	181-200
	type	TRANSMEM
	sequence	MASLGVAAVAIAVLAVLNLC
	description	Helical; Name=6 => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI8

58)	chain	B
	residue	181-200
	type	TRANSMEM
	sequence	MASLGVAAVAIAVLAVLNLC
	description	Helical; Name=6 => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI8

59)	chain	A
	residue	205-222
	type	TRANSMEM
	sequence	TGVYILVGVVLWTAVLKS
	description	Helical; Name=7 => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI9

60)	chain	B
	residue	205-222
	type	TRANSMEM
	sequence	TGVYILVGVVLWTAVLKS
	description	Helical; Name=7 => ECO:0000269\|PubMed:25422503, ECO:0007744\|PDB:4ATV, ECO:0007744\|PDB:4AU5
	source	Swiss-Prot : SWS_FT_FI9