eF-site ID 1x9w-AB PDB Code 1x9w Chain A, B click to enlarge Title T7 DNA polymerase in complex with a primer/template DNA containing a disordered N-2 aminofluorene on the template, crystallized with dideoxy-ATP as the incoming nucleotide. Classification TRANSFERASE/ELECTRON TRANSPORT/DNA Compound 5'-D(*GP*GP*AP*GP*AP*GP*TP*GP*AP*TP*T*GP*GP*T*AP*GP*TP*GP*TP*GP*AP*(2DT))-3' Source null (1X9W) Sequence A:  MIVSDIEANALLESVTKFHCGVIYDYSTAEYVSYRPSDFG AYLDALEAEVARGGLIVFHNGHKYDVPALTKLAKLQLNRE FHLPRENCIDTLVLSRLIHSNLKDTDMGLLRSGKLPGALE AWGYRLGEMKGEYKDDFKRMLEEQGEEYVDGMEWWNFNEE MMDYNVQDVVVTKALLEKLLSDKHYFPPEIDFTDVGYTTF WSESLEAVDIEHRAAWLLAKQERNGFPFDTKAIEELYVEL AARRSELLRKLTETFGSWYQPKGGTEMFCHPRTGKPLPKY PRIKTPKVGGIFCELDTREYVAGAPYTPVEHVVFNPSSRD HIQKKLQEAGWVPTKYTDKGAPVVDDEVLEGVRVDDPEKQ AAIDLIKEYLMIQKRIGQSAEGDKAWLRYVAEDGKIHGSV NPNGAVTGRATHAFPNLAQIPGVRSPYGEQCRAAFGAEHH LDGITGKPWVQAGIDASGLELRCLAHFMARFDNGEYAHEI LNGDIHTKNQIAAELPTRDNAKTFIYGFLYGAGDEKIGQI VGAGKERGKELKKKFLENTPAIAALRESIQQTLVEKWKRR WIKGLDGRKVHVRSPHAALNTLLQSAGALICKLWIIKTEE MLVEKGLKHGWDGDFAYMAWVHDEIQVGCRTEEIAQVVIE TAQEAMRWVGDHWNFRCLLDTEGKMGPNWAICH B:  KIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPIL DEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFK NGEVAATKVGALSKGQLKEFLDANL Description Functional site 1) chain A residue 5 type sequence D description BINDING SITE FOR RESIDUE MG A 3001 source : AC1 2) chain A residue 59 type sequence H description BINDING SITE FOR RESIDUE MG A 3001 source : AC1 3) chain A residue 65 type sequence D description BINDING SITE FOR RESIDUE MG A 3001 source : AC1 4) chain A residue 518-537 type prosite sequence RDNAKTFIYGFLYGAGDEKI description DNA_POLYMERASE_A DNA polymerase family A signature. RdnAKtfiYGflYgaGdekI source prosite : PS00447 5) chain B residue 70 type MOD_RES sequence Y description N6-acetyllysine => ECO:0000269|PubMed:18723842 source Swiss-Prot : SWS_FT_FI4 6) chain B residue 24-42 type prosite sequence LVDFWAEWCGPCKMIAPIL description THIOREDOXIN_1 Thioredoxin family active site. LVdFWaeWCGPCKmIapiL source prosite : PS00194 7) chain B residue 33 type ACT_SITE sequence G description Nucleophile source Swiss-Prot : SWS_FT_FI1 8) chain B residue 36 type ACT_SITE sequence K description Nucleophile source Swiss-Prot : SWS_FT_FI1 9) chain A residue 180 type ACT_SITE sequence A description Nucleophile source Swiss-Prot : SWS_FT_FI1 10) chain B residue 27 type SITE sequence F description Deprotonates C-terminal active site Cys source Swiss-Prot : SWS_FT_FI2 11) chain A residue 660 type SITE sequence C description Deprotonates C-terminal active site Cys source Swiss-Prot : SWS_FT_FI2 12) chain A residue 482 type SITE sequence R description Deprotonates C-terminal active site Cys source Swiss-Prot : SWS_FT_FI2 13) chain B residue 34 type SITE sequence P description Contributes to redox potential value source Swiss-Prot : SWS_FT_FI3 14) chain B residue 35 type SITE sequence C description Contributes to redox potential value source Swiss-Prot : SWS_FT_FI3 15) chain A residue 528 type SITE sequence F description Contributes to redox potential value source Swiss-Prot : SWS_FT_FI3 16) chain A residue 532 type SITE sequence A description Contributes to redox potential value source Swiss-Prot : SWS_FT_FI3

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