|
|
eF-site ID
|
1x9s-B |
|
PDB Code
|
1x9s |
|
Chain
|
B |
|
click to enlarge
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Title
|
T7 DNA polymerase in complex with a primer/template DNA containing a disordered N-2 aminofluorene on the template, crystallized with dideoxy-CTP as the incoming nucleotide. |
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Classification
|
TRANSFERASE/ELECTRON TRANSPORT/DNA |
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Compound
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5'-D(*GP*GP*AP*GP*AP*GP*TP*GP*AP*TP*T*GP*GP*TP*AP*GP*TP*GP*TP*GP*AP*(2DT))-3' |
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Source
|
Enterobacteria phage T7 (Bacteriophage T7) (1X9S) |
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|
Sequence
|
B: |
KIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPIL
DEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFK
NGEVAATKVGALSKGQLKEFLDANL
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Description
|
|
Functional site
|
|
|
1)
|
chain |
B |
| residue |
24-42 |
| type |
prosite |
| sequence |
LVDFWAEWCGPCKMIAPIL
|
| description |
THIOREDOXIN_1 Thioredoxin family active site. LVdFWaeWCGPCKmIapiL
|
| source |
prosite : PS00194
|
|
|
2)
|
chain |
B |
| residue |
33 |
| type |
ACT_SITE |
| sequence |
G
|
| description |
Nucleophile
|
| source |
Swiss-Prot : SWS_FT_FI1
|
|
|
3)
|
chain |
B |
| residue |
36 |
| type |
ACT_SITE |
| sequence |
K
|
| description |
Nucleophile
|
| source |
Swiss-Prot : SWS_FT_FI1
|
|
|
4)
|
chain |
B |
| residue |
27 |
| type |
SITE |
| sequence |
F
|
| description |
Deprotonates C-terminal active site Cys
|
| source |
Swiss-Prot : SWS_FT_FI2
|
|
|
5)
|
chain |
B |
| residue |
34 |
| type |
SITE |
| sequence |
P
|
| description |
Contributes to redox potential value
|
| source |
Swiss-Prot : SWS_FT_FI3
|
|
|
6)
|
chain |
B |
| residue |
35 |
| type |
SITE |
| sequence |
C
|
| description |
Contributes to redox potential value
|
| source |
Swiss-Prot : SWS_FT_FI3
|
|
|
7)
|
chain |
B |
| residue |
70 |
| type |
MOD_RES |
| sequence |
Y
|
| description |
N6-acetyllysine => ECO:0000269|PubMed:18723842
|
| source |
Swiss-Prot : SWS_FT_FI4
|
|
|
|