eF-site/Summary 1u77-A

eF-site ID	1u77-A
PDB Code	1u77
Chain	A

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Title	Crystal Structure of Ammonia Channel AmtB from E. Coli
Classification	TRANSPORT PROTEIN
Compound	Probable ammonium transporter
Source	null (AMTB_ECOLI)

Sequence	A:	AVADKADNAFXXICTALVLFXTIPGIALFYGGLIRGKNVL SXLTQVTVTFALVCILWVVYGYSLASGEGNNFFGNINWLX LKNIELTAVXGSIYQYIHVAFQGSFACITVGLIVGALAER IRFPAVLIFVVVWLTLSYIPIAHXVWGGGLLASHGALDFA GGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPHNLPXVF TGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAI LGWIFGEWALRGLPSLLGACSGAIAGLVGVTPACGYIGVG GALIIGVVAGLAGLWGVTXLKRLLRVDDPCDVFGVHGVCG IVGCIXTGIFAASSLGGVGFAEGVTXGHQLLVQLESIAIT IVWSGVVAFIGYKLADLTVGLRV

Description

Functional site


1)	chain	A
	residue	69-97
	type	TOPO_DOM
	sequence	GEGNNFFGNINWLXLKNIELTAVXGSIYQ
	description	Periplasmic => ECO:0000269\|PubMed:15361618, ECO:0000269\|PubMed:15563598, ECO:0000269\|PubMed:17040913
	source	Swiss-Prot : SWS_FT_FI1

2)	chain	A
	residue	150-163
	type	TOPO_DOM
	sequence	GGLLASHGALDFAG
	description	Periplasmic => ECO:0000269\|PubMed:15361618, ECO:0000269\|PubMed:15563598, ECO:0000269\|PubMed:17040913
	source	Swiss-Prot : SWS_FT_FI1

3)	chain	A
	residue	220-226
	type	TOPO_DOM
	sequence	AGTANEI
	description	Periplasmic => ECO:0000269\|PubMed:15361618, ECO:0000269\|PubMed:15563598, ECO:0000269\|PubMed:17040913
	source	Swiss-Prot : SWS_FT_FI1

4)	chain	A
	residue	279-280
	type	TOPO_DOM
	sequence	IG
	description	Periplasmic => ECO:0000269\|PubMed:15361618, ECO:0000269\|PubMed:15563598, ECO:0000269\|PubMed:17040913
	source	Swiss-Prot : SWS_FT_FI1

5)	chain	A
	residue	334-348
	type	TOPO_DOM
	sequence	ASSLGGVGFAEGVTX
	description	Periplasmic => ECO:0000269\|PubMed:15361618, ECO:0000269\|PubMed:15563598, ECO:0000269\|PubMed:17040913
	source	Swiss-Prot : SWS_FT_FI1

6)	chain	A
	residue	11-32
	type	TRANSMEM
	sequence	AFXXICTALVLFXTIPGIALFY
	description	Helical => ECO:0000269\|PubMed:15361618, ECO:0000269\|PubMed:15563598, ECO:0000269\|PubMed:17040913
	source	Swiss-Prot : SWS_FT_FI2

7)	chain	A
	residue	44-68
	type	TRANSMEM
	sequence	XLTQVTVTFALVCILWVVYGYSLAS
	description	Helical => ECO:0000269\|PubMed:15361618, ECO:0000269\|PubMed:15563598, ECO:0000269\|PubMed:17040913
	source	Swiss-Prot : SWS_FT_FI2

8)	chain	A
	residue	98-120
	type	TRANSMEM
	sequence	YIHVAFQGSFACITVGLIVGALA
	description	Helical => ECO:0000269\|PubMed:15361618, ECO:0000269\|PubMed:15563598, ECO:0000269\|PubMed:17040913
	source	Swiss-Prot : SWS_FT_FI2

9)	chain	A
	residue	125-149
	type	TRANSMEM
	sequence	FPAVLIFVVVWLTLSYIPIAHXVWG
	description	Helical => ECO:0000269\|PubMed:15361618, ECO:0000269\|PubMed:15563598, ECO:0000269\|PubMed:17040913
	source	Swiss-Prot : SWS_FT_FI2

10)	chain	A
	residue	164-179
	type	TRANSMEM
	sequence	GTVVHINAAIAGLVGA
	description	Helical => ECO:0000269\|PubMed:15361618, ECO:0000269\|PubMed:15563598, ECO:0000269\|PubMed:17040913
	source	Swiss-Prot : SWS_FT_FI2

11)	chain	A
	residue	200-219
	type	TRANSMEM
	sequence	XVFTGTAILYIGWFGFNAGS
	description	Helical => ECO:0000269\|PubMed:15361618, ECO:0000269\|PubMed:15563598, ECO:0000269\|PubMed:17040913
	source	Swiss-Prot : SWS_FT_FI2

12)	chain	A
	residue	227-251
	type	TRANSMEM
	sequence	AALAFVNTVVATAAAILGWIFGEWA
	description	Helical => ECO:0000269\|PubMed:15361618, ECO:0000269\|PubMed:15563598, ECO:0000269\|PubMed:17040913
	source	Swiss-Prot : SWS_FT_FI2

13)	chain	A
	residue	258-278
	type	TRANSMEM
	sequence	LLGACSGAIAGLVGVTPACGY
	description	Helical => ECO:0000269\|PubMed:15361618, ECO:0000269\|PubMed:15563598, ECO:0000269\|PubMed:17040913
	source	Swiss-Prot : SWS_FT_FI2

14)	chain	A
	residue	281-299
	type	TRANSMEM
	sequence	VGGALIIGVVAGLAGLWGV
	description	Helical => ECO:0000269\|PubMed:15361618, ECO:0000269\|PubMed:15563598, ECO:0000269\|PubMed:17040913
	source	Swiss-Prot : SWS_FT_FI2

15)	chain	A
	residue	312-333
	type	TRANSMEM
	sequence	CDVFGVHGVCGIVGCIXTGIFA
	description	Helical => ECO:0000269\|PubMed:15361618, ECO:0000269\|PubMed:15563598, ECO:0000269\|PubMed:17040913
	source	Swiss-Prot : SWS_FT_FI2

16)	chain	A
	residue	349-377
	type	TRANSMEM
	sequence	GHQLLVQLESIAITIVWSGVVAFIGYKLA
	description	Helical => ECO:0000269\|PubMed:15361618, ECO:0000269\|PubMed:15563598, ECO:0000269\|PubMed:17040913
	source	Swiss-Prot : SWS_FT_FI2

17)	chain	A
	residue	33-43
	type	TOPO_DOM
	sequence	GGLIRGKNVLS
	description	Cytoplasmic => ECO:0000269\|PubMed:15361618, ECO:0000269\|PubMed:15563598, ECO:0000269\|PubMed:17040913
	source	Swiss-Prot : SWS_FT_FI3

18)	chain	A
	residue	121-124
	type	TOPO_DOM
	sequence	ERIR
	description	Cytoplasmic => ECO:0000269\|PubMed:15361618, ECO:0000269\|PubMed:15563598, ECO:0000269\|PubMed:17040913
	source	Swiss-Prot : SWS_FT_FI3

19)	chain	A
	residue	180-199
	type	TOPO_DOM
	sequence	YLIGKRVGFGKEAFKPHNLP
	description	Cytoplasmic => ECO:0000269\|PubMed:15361618, ECO:0000269\|PubMed:15563598, ECO:0000269\|PubMed:17040913
	source	Swiss-Prot : SWS_FT_FI3

20)	chain	A
	residue	252-257
	type	TOPO_DOM
	sequence	LRGLPS
	description	Cytoplasmic => ECO:0000269\|PubMed:15361618, ECO:0000269\|PubMed:15563598, ECO:0000269\|PubMed:17040913
	source	Swiss-Prot : SWS_FT_FI3

21)	chain	A
	residue	300-311
	type	TOPO_DOM
	sequence	TXLKRLLRVDDP
	description	Cytoplasmic => ECO:0000269\|PubMed:15361618, ECO:0000269\|PubMed:15563598, ECO:0000269\|PubMed:17040913
	source	Swiss-Prot : SWS_FT_FI3

22)	chain	A
	residue	219
	type	BINDING
	sequence	S
	description	BINDING => ECO:0000269\|PubMed:15361618
	source	Swiss-Prot : SWS_FT_FI4

23)	chain	A
	residue	160
	type	SITE
	sequence	D
	description	Important for the deprotonation of the ammonium cation => ECO:0000269\|PubMed:19278252
	source	Swiss-Prot : SWS_FT_FI5

24)	chain	A
	residue	318
	type	SITE
	sequence	H
	description	Twin-His motif. Important for optimum substrate conductance => ECO:0000305\|PubMed:17040913, ECO:0000305\|PubMed:23667517, ECO:0000305\|PubMed:32662768
	source	Swiss-Prot : SWS_FT_FI6

25)	chain	A
	residue	168
	type	SITE
	sequence	H
	description	Twin-His motif. Important for optimum substrate conductance => ECO:0000305\|PubMed:17040913, ECO:0000305\|PubMed:23667517, ECO:0000305\|PubMed:32662768
	source	Swiss-Prot : SWS_FT_FI6

26)	chain	A
	residue	215
	type	SITE
	sequence	F
	description	Important for optimum substrate conductance => ECO:0000305\|PubMed:18362341
	source	Swiss-Prot : SWS_FT_FI7

27)	chain	A
	residue	160-185
	type	prosite
	sequence	DFAGGTVVHINAAIAGLVGAYLIGKR
	description	AMMONIUM_TRANSP Ammonium transporters signature. DFAGGtvVhinAAiaGLvgaYLiGkR
	source	prosite : PS01219