eF-site ID 1t8e-B
PDB Code 1t8e
Chain B

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Title T7 DNA Polymerase Ternary Complex with dCTP at the Insertion Site.
Classification transferase/electron transport/DNA
Compound 5'-D(P*CP*GP*AP*AP*AP*AP*CP*GP*AP*CP*GP*GP*CP*CP*AP*GP*TP*GP*CP*CP*AP*(2DT))-3'
Source Enterobacteria phage T7 (Bacteriophage T7) (1T8E)
Sequence B:  DKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPI
LDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLF
KNGEVAATKVGALSKGQLKEFLDANL
Description


Functional site

1) chain B
residue 5
type
sequence I
description BINDING SITE FOR RESIDUE MES A 4008
source : AC6

2) chain B
residue 6
type
sequence H
description BINDING SITE FOR RESIDUE MES A 4008
source : AC6

3) chain B
residue 11
type
sequence S
description BINDING SITE FOR RESIDUE MES A 4008
source : AC6

4) chain B
residue 14
type
sequence T
description BINDING SITE FOR RESIDUE MES A 4008
source : AC6

5) chain B
residue 15
type
sequence D
description BINDING SITE FOR RESIDUE MES A 4008
source : AC6

6) chain B
residue 70
type MOD_RES
sequence Y
description N6-acetyllysine => ECO:0000269|PubMed:18723842
source Swiss-Prot : SWS_FT_FI4

7) chain B
residue 33
type ACT_SITE
sequence G
description Nucleophile
source Swiss-Prot : SWS_FT_FI1

8) chain B
residue 36
type ACT_SITE
sequence K
description Nucleophile
source Swiss-Prot : SWS_FT_FI1

9) chain B
residue 27
type SITE
sequence F
description Deprotonates C-terminal active site Cys
source Swiss-Prot : SWS_FT_FI2

10) chain B
residue 34
type SITE
sequence P
description Contributes to redox potential value
source Swiss-Prot : SWS_FT_FI3

11) chain B
residue 35
type SITE
sequence C
description Contributes to redox potential value
source Swiss-Prot : SWS_FT_FI3

12) chain B
residue 24-42
type prosite
sequence LVDFWAEWCGPCKMIAPIL
description THIOREDOXIN_1 Thioredoxin family active site. LVdFWaeWCGPCKmIapiL
source prosite : PS00194


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