eF-site ID 1t1i-A PDB Code 1t1i Chain A click to enlarge Title High Resolution Crystal Structure of Mutant W129A of Kumamolisin, a Sedolisin Type Proteinase (previously called Kumamolysin or KSCP) Classification HYDROLASE Compound kumamolisin Source Bacillus sp. MN-32 (Q8RR56_9BACI) Sequence A:  AAPTAYTPLDVAQAYQFPEGLDGQGQCIAIIELGGGYDET SLAQYFASLGVSAPQVVSVSVDGATNQPTGDPNGPDGEVE LDIEVAGALAPGAKIAVYFAPNTDAGFLNAITTAVHDPTH KPSIVSISAGGPEDSWAPASIAAMNRAFLDAAALGVTVLA AAGDSGSTDGEQDGLYHVDFPAASPYVLACGGTRLVASAG RIERETVWNDGPDGGSTGGGVSRIFPLPSWQERANVPPSA NPGAGSGRGVPDVAGNADPATGYEVVIDGETTVIGGTSAV APLFAALVARINQKLGKPVGYLNPTLYQLPPEVFHDITEG NNDIANRARIYQAGPGWDPCTGLGSPIGIRLLQALLP Description Functional site 1) chain A residue 316 type sequence D description BINDING SITE FOR RESIDUE CA A 365 source : AC1 2) chain A residue 317 type sequence I description BINDING SITE FOR RESIDUE CA A 365 source : AC1 3) chain A residue 334 type sequence G description BINDING SITE FOR RESIDUE CA A 365 source : AC1 4) chain A residue 336 type sequence G description BINDING SITE FOR RESIDUE CA A 365 source : AC1 5) chain A residue 338 type sequence D description BINDING SITE FOR RESIDUE CA A 365 source : AC1 6) chain A residue 145 type sequence N description BINDING SITE FOR RESIDUE SO4 A 366 source : AC2 7) chain A residue 146 type sequence R description BINDING SITE FOR RESIDUE SO4 A 366 source : AC2 8) chain A residue 149 type sequence L description BINDING SITE FOR RESIDUE SO4 A 366 source : AC2 9) chain A residue 186 type sequence Y description BINDING SITE FOR RESIDUE SO4 A 366 source : AC2 10) chain A residue 198 type sequence S description BINDING SITE FOR RESIDUE SO4 A 366 source : AC2 11) chain A residue 199 type sequence A description BINDING SITE FOR RESIDUE SO4 A 366 source : AC2 12) chain A residue 225 type sequence F description BINDING SITE FOR RESIDUE SO4 A 366 source : AC2 13) chain A residue 226 type sequence P description BINDING SITE FOR RESIDUE SO4 A 366 source : AC2 14) chain A residue 121 type sequence K description BINDING SITE FOR RESIDUE SO4 A 367 source : AC3 15) chain A residue 327 type sequence R description BINDING SITE FOR RESIDUE SO4 A 367 source : AC3 16) chain A residue 329 type sequence R description BINDING SITE FOR RESIDUE SO4 A 367 source : AC3

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