eF-site ID 1sub-A
PDB Code 1sub
Chain A

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Title CALCIUM-INDEPENDENT SUBTILISIN BY DESIGN
Classification HYDROLASE(SERINE PROTEINASE)
Compound SUBTILISIN BPN' CRB-S3
Source Bacillus amyloliquefaciens (Bacillus velezensis) (SUBT_BACAM)
Sequence A:  AQSVPYGVSQIKAPALHSQGYTGSNVKVAVIDSGIDSSHP
DLKVAGGASMVPSETNPFQDNNSHGTHVAGTVAALNNSIG
VLGVAPSASLYAVKVLGADGSGQYSWIINGIEWAIANNMD
VINMSLGGPSGSAALKAAVDKAVASGVVVVAAAGNEGTSG
SSSTVGYPGKYPSVIAVGAVDSSNQRASFSSVGPELDVMA
PGVSIQSTLPGNKYGAYSGTXMASPHVAGAAALILSKHPN
WTNTQVRSSLENTTTKLGDSFYYGKGLINVQAAAQ
Description


Functional site

1) chain A
residue 32
type
sequence D
description ACTIVE SITE, THE CATALYTIC TRIAD
source : ACT

2) chain A
residue 64
type
sequence H
description ACTIVE SITE, THE CATALYTIC TRIAD
source : ACT

3) chain A
residue 221
type
sequence X
description ACTIVE SITE, THE CATALYTIC TRIAD
source : ACT

4) chain A
residue 2
type
sequence Q
description BINDING SITE FOR RESIDUE CA A 295
source : AC1

5) chain A
residue 41
type
sequence D
description BINDING SITE FOR RESIDUE CA A 295
source : AC1

6) chain A
residue 75
type
sequence L
description BINDING SITE FOR RESIDUE CA A 295
source : AC1

7) chain A
residue 77
type
sequence N
description BINDING SITE FOR RESIDUE CA A 295
source : AC1

8) chain A
residue 79
type
sequence I
description BINDING SITE FOR RESIDUE CA A 295
source : AC1

9) chain A
residue 81
type
sequence V
description BINDING SITE FOR RESIDUE CA A 295
source : AC1

10) chain A
residue 169
type
sequence G
description BINDING SITE FOR RESIDUE K A 297
source : AC2

11) chain A
residue 171
type
sequence Y
description BINDING SITE FOR RESIDUE K A 297
source : AC2

12) chain A
residue 174
type
sequence V
description BINDING SITE FOR RESIDUE K A 297
source : AC2

13) chain A
residue 195
type
sequence E
description BINDING SITE FOR RESIDUE K A 297
source : AC2

14) chain A
residue 197
type
sequence D
description BINDING SITE FOR RESIDUE K A 297
source : AC2

15) chain A
residue 37
type
sequence S
description BINDING SITE FOR RESIDUE ACN A 298
source : AC3

16) chain A
residue 58
type
sequence F
description BINDING SITE FOR RESIDUE ACN A 298
source : AC3

17) chain A
residue 186
type
sequence R
description BINDING SITE FOR RESIDUE ACN A 298
source : AC3

18) chain A
residue 32
type catalytic
sequence D
description 723
source MCSA : MCSA1

19) chain A
residue 64
type catalytic
sequence H
description 723
source MCSA : MCSA1

20) chain A
residue 155
type catalytic
sequence N
description 723
source MCSA : MCSA1

21) chain A
residue 221
type catalytic
sequence X
description 723
source MCSA : MCSA1

22) chain A
residue 28-39
type prosite
sequence VAVIDSGIDSSH
description SUBTILASE_ASP Serine proteases, subtilase family, aspartic acid active site. VAVIDSGIdssH
source prosite : PS00136

23) chain A
residue 64-74
type prosite
sequence HGTHVAGTVAA
description SUBTILASE_HIS Serine proteases, subtilase family, histidine active site. HGThVAGtVAA
source prosite : PS00137

24) chain A
residue 32
type ACT_SITE
sequence D
description Charge relay system => ECO:0000255|PROSITE-ProRule:PRU01240, ECO:0000269|PubMed:5249818
source Swiss-Prot : SWS_FT_FI1

25) chain A
residue 64
type ACT_SITE
sequence H
description Charge relay system => ECO:0000255|PROSITE-ProRule:PRU01240, ECO:0000269|PubMed:5249818
source Swiss-Prot : SWS_FT_FI1

26) chain A
residue 221
type ACT_SITE
sequence X
description Charge relay system => ECO:0000255|PROSITE-ProRule:PRU01240, ECO:0000269|PubMed:5249818
source Swiss-Prot : SWS_FT_FI1

27) chain A
residue 2
type BINDING
sequence Q
description
source Swiss-Prot : SWS_FT_FI2

28) chain A
residue 41
type BINDING
sequence D
description
source Swiss-Prot : SWS_FT_FI2

29) chain A
residue 75
type BINDING
sequence L
description
source Swiss-Prot : SWS_FT_FI2

30) chain A
residue 77
type BINDING
sequence N
description
source Swiss-Prot : SWS_FT_FI2

31) chain A
residue 79
type BINDING
sequence I
description
source Swiss-Prot : SWS_FT_FI2

32) chain A
residue 81
type BINDING
sequence V
description
source Swiss-Prot : SWS_FT_FI2

33) chain A
residue 169
type BINDING
sequence G
description
source Swiss-Prot : SWS_FT_FI2

34) chain A
residue 171
type BINDING
sequence Y
description
source Swiss-Prot : SWS_FT_FI2

35) chain A
residue 174
type BINDING
sequence V
description
source Swiss-Prot : SWS_FT_FI2


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