|
|
eF-site ID
|
1qal-B |
|
PDB Code
|
1qal |
|
Chain
|
B |
|
click to enlarge
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Title
|
THE ACTIVE SITE BASE CONTROLS COFACTOR REACTIVITY IN ESCHERICHIA COLI AMINE OXIDASE : X-RAY CRYSTALLOGRAPHIC STUDIES WITH MUTATIONAL VARIANTS |
|
Classification
|
OXIDOREDUCTASE |
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Compound
|
COPPER AMINE OXIDASE |
|
Source
|
Escherichia coli (strain K12) (AMO_ECOLI) |
|
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Sequence
|
B: |
HMVPMDKTLKEFGADVQWDDYAQLFTLIKDGAYVKVKPGA
QTAIVNGQPLALQVPVVMKDNKAWVSDTFINDVFQSGLDQ
TFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTE
ISLLPPDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEA
VVDLQNNKLLSWQPIKDAHGMVLLDDFASVQNIINNSEEF
AAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKV
ISYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVP
VPMTARPFDGRDRVAPAVKPMQIIEPEGKNYTITGDMIHW
RNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSLGGM
IVPYGDPDIGWYFKAYLNSGDYGMGTLTSPIARGKDAPSN
AVLLNETIADYTGVPMEIPRAIAVFERYAGPEYKHQEMGQ
PNVSTERRELVVRWISTVGNXDYIFDWIFHENGTIGIDAG
ATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQ
HIYNFRLDLDVDGENNSLVAMDPVVKPNTAGGPRTSTMQV
NQYNIGNEQDAAQKFDPGTIRLLSNPNKENRMGNPVSYQI
IPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHP
GERFPEGKYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTT
GTTHVARAEEWPIMPTEWVHTLLKPWNFFDETPTLGALKK
D
|
|
|
Description
|
|
Functional site
|
|
|
1)
|
chain |
B |
| residue |
524 |
| type |
|
| sequence |
H
|
| description |
BINDING SITE FOR RESIDUE CU B 801
|
| source |
: AC4
|
|
|
2)
|
chain |
B |
| residue |
526 |
| type |
|
| sequence |
H
|
| description |
BINDING SITE FOR RESIDUE CU B 801
|
| source |
: AC4
|
|
|
3)
|
chain |
B |
| residue |
689 |
| type |
|
| sequence |
H
|
| description |
BINDING SITE FOR RESIDUE CU B 801
|
| source |
: AC4
|
|
|
4)
|
chain |
B |
| residue |
133 |
| type |
|
| sequence |
K
|
| description |
BINDING SITE FOR RESIDUE CA B 802
|
| source |
: AC5
|
|
|
5)
|
chain |
B |
| residue |
533 |
| type |
|
| sequence |
D
|
| description |
BINDING SITE FOR RESIDUE CA B 802
|
| source |
: AC5
|
|
|
6)
|
chain |
B |
| residue |
534 |
| type |
|
| sequence |
L
|
| description |
BINDING SITE FOR RESIDUE CA B 802
|
| source |
: AC5
|
|
|
7)
|
chain |
B |
| residue |
535 |
| type |
|
| sequence |
D
|
| description |
BINDING SITE FOR RESIDUE CA B 802
|
| source |
: AC5
|
|
|
8)
|
chain |
B |
| residue |
678 |
| type |
|
| sequence |
D
|
| description |
BINDING SITE FOR RESIDUE CA B 802
|
| source |
: AC5
|
|
|
9)
|
chain |
B |
| residue |
679 |
| type |
|
| sequence |
A
|
| description |
BINDING SITE FOR RESIDUE CA B 802
|
| source |
: AC5
|
|
|
10)
|
chain |
B |
| residue |
573 |
| type |
|
| sequence |
E
|
| description |
BINDING SITE FOR RESIDUE CA B 803
|
| source |
: AC6
|
|
|
11)
|
chain |
B |
| residue |
667 |
| type |
|
| sequence |
Y
|
| description |
BINDING SITE FOR RESIDUE CA B 803
|
| source |
: AC6
|
|
|
12)
|
chain |
B |
| residue |
672 |
| type |
|
| sequence |
E
|
| description |
BINDING SITE FOR RESIDUE CA B 803
|
| source |
: AC6
|
|
|
13)
|
chain |
B |
| residue |
369 |
| type |
catalytic |
| sequence |
Y
|
| description |
864
|
| source |
MCSA : MCSA2
|
|
|
14)
|
chain |
B |
| residue |
383 |
| type |
catalytic |
| sequence |
N
|
| description |
864
|
| source |
MCSA : MCSA2
|
|
|
15)
|
chain |
B |
| residue |
466 |
| type |
catalytic |
| sequence |
X
|
| description |
864
|
| source |
MCSA : MCSA2
|
|
|
16)
|
chain |
B |
| residue |
524 |
| type |
catalytic |
| sequence |
H
|
| description |
864
|
| source |
MCSA : MCSA2
|
|
|
17)
|
chain |
B |
| residue |
526 |
| type |
catalytic |
| sequence |
H
|
| description |
864
|
| source |
MCSA : MCSA2
|
|
|
18)
|
chain |
B |
| residue |
689 |
| type |
catalytic |
| sequence |
H
|
| description |
864
|
| source |
MCSA : MCSA2
|
|
|
19)
|
chain |
B |
| residue |
466 |
| type |
MOD_RES |
| sequence |
X
|
| description |
2',4',5'-topaquinone => ECO:0000269|PubMed:10387067, ECO:0007744|PDB:1DYU, ECO:0007744|PDB:1JRQ, ECO:0007744|PDB:1QAF, ECO:0007744|PDB:1QAK, ECO:0007744|PDB:1QAL
|
| source |
Swiss-Prot : SWS_FT_FI11
|
|
|
20)
|
chain |
B |
| residue |
679 |
| type |
BINDING |
| sequence |
A
|
| description |
|
| source |
Swiss-Prot : SWS_FT_FI10
|
|
|
21)
|
chain |
B |
| residue |
533 |
| type |
BINDING |
| sequence |
D
|
| description |
BINDING => ECO:0000250|UniProtKB:Q43077
|
| source |
Swiss-Prot : SWS_FT_FI6
|
|
|
22)
|
chain |
B |
| residue |
535 |
| type |
BINDING |
| sequence |
D
|
| description |
BINDING => ECO:0000250|UniProtKB:Q43077
|
| source |
Swiss-Prot : SWS_FT_FI6
|
|
|
23)
|
chain |
B |
| residue |
678 |
| type |
BINDING |
| sequence |
D
|
| description |
BINDING => ECO:0000250|UniProtKB:Q43077
|
| source |
Swiss-Prot : SWS_FT_FI6
|
|
|
24)
|
chain |
B |
| residue |
670 |
| type |
BINDING |
| sequence |
D
|
| description |
BINDING => ECO:0000269|PubMed:10576737, ECO:0007744|PDB:1D6Y, ECO:0007744|PDB:1D6Z, ECO:0007744|PDB:1DYU, ECO:0007744|PDB:1OAC, ECO:0007744|PDB:1QAK, ECO:0007744|PDB:1QAL, ECO:0007744|PDB:2WGQ
|
| source |
Swiss-Prot : SWS_FT_FI9
|
|
|
25)
|
chain |
B |
| residue |
383 |
| type |
ACT_SITE |
| sequence |
N
|
| description |
Proton acceptor => ECO:0000269|PubMed:10387067, ECO:0000269|PubMed:9048544, ECO:0007744|PDB:1OAC
|
| source |
Swiss-Prot : SWS_FT_FI1
|
|
|
26)
|
chain |
B |
| residue |
466 |
| type |
ACT_SITE |
| sequence |
X
|
| description |
Schiff-base intermediate with substrate; via topaquinone => ECO:0000269|PubMed:10387067, ECO:0007744|PDB:1OAC, ECO:0007744|PDB:1QAK, ECO:0007744|PDB:2WGQ, ECO:0007744|PDB:2WO0, ECO:0007744|PDB:2WOF
|
| source |
Swiss-Prot : SWS_FT_FI2
|
|
|
27)
|
chain |
B |
| residue |
381 |
| type |
BINDING |
| sequence |
Y
|
| description |
BINDING => ECO:0000269|PubMed:10576737, ECO:0007744|PDB:1D6U, ECO:0007744|PDB:1D6Y, ECO:0007744|PDB:1D6Z
|
| source |
Swiss-Prot : SWS_FT_FI3
|
|
|
28)
|
chain |
B |
| residue |
463 |
| type |
BINDING |
| sequence |
V
|
| description |
BINDING => ECO:0000269|PubMed:10576737, ECO:0000269|PubMed:9048544, ECO:0007744|PDB:1D6U, ECO:0007744|PDB:1D6Y, ECO:0007744|PDB:1D6Z, ECO:0007744|PDB:1LVN, ECO:0007744|PDB:1SPU
|
| source |
Swiss-Prot : SWS_FT_FI4
|
|
|
29)
|
chain |
B |
| residue |
524 |
| type |
BINDING |
| sequence |
H
|
| description |
BINDING => ECO:0000269|PubMed:10387067, ECO:0000269|PubMed:10576737, ECO:0000269|PubMed:9048544, ECO:0007744|PDB:1D6U, ECO:0007744|PDB:1D6Y, ECO:0007744|PDB:1D6Z, ECO:0007744|PDB:1DYU, ECO:0007744|PDB:1JRQ, ECO:0007744|PDB:1LVN, ECO:0007744|PDB:1OAC, ECO:0007744|PDB:1QAF, ECO:0007744|PDB:1QAK, ECO:0007744|PDB:1QAL, ECO:0007744|PDB:1SPU, ECO:0007744|PDB:2W0Q, ECO:0007744|PDB:2WGQ, ECO:0007744|PDB:2WO0, ECO:0007744|PDB:2WOF, ECO:0007744|PDB:2WOH
|
| source |
Swiss-Prot : SWS_FT_FI5
|
|
|
30)
|
chain |
B |
| residue |
526 |
| type |
BINDING |
| sequence |
H
|
| description |
BINDING => ECO:0000269|PubMed:10387067, ECO:0000269|PubMed:10576737, ECO:0000269|PubMed:9048544, ECO:0007744|PDB:1D6U, ECO:0007744|PDB:1D6Y, ECO:0007744|PDB:1D6Z, ECO:0007744|PDB:1DYU, ECO:0007744|PDB:1JRQ, ECO:0007744|PDB:1LVN, ECO:0007744|PDB:1OAC, ECO:0007744|PDB:1QAF, ECO:0007744|PDB:1QAK, ECO:0007744|PDB:1QAL, ECO:0007744|PDB:1SPU, ECO:0007744|PDB:2W0Q, ECO:0007744|PDB:2WGQ, ECO:0007744|PDB:2WO0, ECO:0007744|PDB:2WOF, ECO:0007744|PDB:2WOH
|
| source |
Swiss-Prot : SWS_FT_FI5
|
|
|
31)
|
chain |
B |
| residue |
689 |
| type |
BINDING |
| sequence |
H
|
| description |
BINDING => ECO:0000269|PubMed:10387067, ECO:0000269|PubMed:10576737, ECO:0000269|PubMed:9048544, ECO:0007744|PDB:1D6U, ECO:0007744|PDB:1D6Y, ECO:0007744|PDB:1D6Z, ECO:0007744|PDB:1DYU, ECO:0007744|PDB:1JRQ, ECO:0007744|PDB:1LVN, ECO:0007744|PDB:1OAC, ECO:0007744|PDB:1QAF, ECO:0007744|PDB:1QAK, ECO:0007744|PDB:1QAL, ECO:0007744|PDB:1SPU, ECO:0007744|PDB:2W0Q, ECO:0007744|PDB:2WGQ, ECO:0007744|PDB:2WO0, ECO:0007744|PDB:2WOF, ECO:0007744|PDB:2WOH
|
| source |
Swiss-Prot : SWS_FT_FI5
|
|
|
32)
|
chain |
B |
| residue |
534 |
| type |
BINDING |
| sequence |
L
|
| description |
BINDING => ECO:0000269|PubMed:10387067, ECO:0000269|PubMed:10576737, ECO:0000269|PubMed:9048544, ECO:0007744|PDB:1D6U, ECO:0007744|PDB:1D6Y, ECO:0007744|PDB:1D6Z, ECO:0007744|PDB:1DYU, ECO:0007744|PDB:1JRQ, ECO:0007744|PDB:1LVN, ECO:0007744|PDB:1OAC, ECO:0007744|PDB:1QAF, ECO:0007744|PDB:1QAK, ECO:0007744|PDB:1QAL, ECO:0007744|PDB:1SPU, ECO:0007744|PDB:2W0Q, ECO:0007744|PDB:2WGQ, ECO:0007744|PDB:2WOH
|
| source |
Swiss-Prot : SWS_FT_FI7
|
|
|
33)
|
chain |
B |
| residue |
573 |
| type |
BINDING |
| sequence |
E
|
| description |
BINDING => ECO:0000269|PubMed:10387067, ECO:0000269|PubMed:10576737, ECO:0000269|PubMed:9048544, ECO:0007744|PDB:1D6U, ECO:0007744|PDB:1D6Y, ECO:0007744|PDB:1D6Z, ECO:0007744|PDB:1DYU, ECO:0007744|PDB:1JRQ, ECO:0007744|PDB:1LVN, ECO:0007744|PDB:1OAC, ECO:0007744|PDB:1QAF, ECO:0007744|PDB:1QAK, ECO:0007744|PDB:1QAL, ECO:0007744|PDB:1SPU, ECO:0007744|PDB:2W0Q, ECO:0007744|PDB:2WGQ
|
| source |
Swiss-Prot : SWS_FT_FI8
|
|
|
34)
|
chain |
B |
| residue |
667 |
| type |
BINDING |
| sequence |
Y
|
| description |
BINDING => ECO:0000269|PubMed:10387067, ECO:0000269|PubMed:10576737, ECO:0000269|PubMed:9048544, ECO:0007744|PDB:1D6U, ECO:0007744|PDB:1D6Y, ECO:0007744|PDB:1D6Z, ECO:0007744|PDB:1DYU, ECO:0007744|PDB:1JRQ, ECO:0007744|PDB:1LVN, ECO:0007744|PDB:1OAC, ECO:0007744|PDB:1QAF, ECO:0007744|PDB:1QAK, ECO:0007744|PDB:1QAL, ECO:0007744|PDB:1SPU, ECO:0007744|PDB:2W0Q, ECO:0007744|PDB:2WGQ
|
| source |
Swiss-Prot : SWS_FT_FI8
|
|
|
35)
|
chain |
B |
| residue |
672 |
| type |
BINDING |
| sequence |
E
|
| description |
BINDING => ECO:0000269|PubMed:10387067, ECO:0000269|PubMed:10576737, ECO:0000269|PubMed:9048544, ECO:0007744|PDB:1D6U, ECO:0007744|PDB:1D6Y, ECO:0007744|PDB:1D6Z, ECO:0007744|PDB:1DYU, ECO:0007744|PDB:1JRQ, ECO:0007744|PDB:1LVN, ECO:0007744|PDB:1OAC, ECO:0007744|PDB:1QAF, ECO:0007744|PDB:1QAK, ECO:0007744|PDB:1QAL, ECO:0007744|PDB:1SPU, ECO:0007744|PDB:2W0Q, ECO:0007744|PDB:2WGQ
|
| source |
Swiss-Prot : SWS_FT_FI8
|
|
|
|