|
|
eF-site ID
|
1otx-B |
|
PDB Code
|
1otx |
|
Chain
|
B |
|
click to enlarge
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Title
|
Purine Nucleoside Phosphorylase M64V mutant |
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Classification
|
TRANSFERASE |
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Compound
|
Purine nucleoside phosphorylase |
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Source
|
Escherichia coli (strain K12) (DEOD_ECOLI) |
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Sequence
|
B: |
ATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVN
NVRGMLGFTGTYKGRKISVMGHGVGIPSCSIYTKELITDF
GVKKIIRVGSCGAVLPHVKLRDVVIGMGACTDSKVNRIRF
KDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLFY
SPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTIC
TVSDHIRTHEQTTAAERQTTFNDMIKIALESVLLGDK
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Description
|
|
Functional site
|
|
|
1)
|
chain |
B |
| residue |
20 |
| type |
|
| sequence |
G
|
| description |
BINDING SITE FOR RESIDUE PO4 B 248
|
| source |
: AC2
|
|
|
2)
|
chain |
B |
| residue |
87 |
| type |
|
| sequence |
R
|
| description |
BINDING SITE FOR RESIDUE PO4 B 248
|
| source |
: AC2
|
|
|
3)
|
chain |
B |
| residue |
89 |
| type |
|
| sequence |
G
|
| description |
BINDING SITE FOR RESIDUE PO4 B 248
|
| source |
: AC2
|
|
|
4)
|
chain |
B |
| residue |
90 |
| type |
|
| sequence |
S
|
| description |
BINDING SITE FOR RESIDUE PO4 B 248
|
| source |
: AC2
|
|
|
5)
|
chain |
B |
| residue |
43 |
| type |
|
| sequence |
R
|
| description |
BINDING SITE FOR RESIDUE PO4 C 249
|
| source |
: AC3
|
|
|
6)
|
chain |
B |
| residue |
21 |
| type |
catalytic |
| sequence |
D
|
| description |
375
|
| source |
MCSA : MCSA2
|
|
|
7)
|
chain |
B |
| residue |
25 |
| type |
catalytic |
| sequence |
A
|
| description |
375
|
| source |
MCSA : MCSA2
|
|
|
8)
|
chain |
B |
| residue |
44 |
| type |
catalytic |
| sequence |
G
|
| description |
375
|
| source |
MCSA : MCSA2
|
|
|
9)
|
chain |
B |
| residue |
88 |
| type |
catalytic |
| sequence |
V
|
| description |
375
|
| source |
MCSA : MCSA2
|
|
|
10)
|
chain |
B |
| residue |
91 |
| type |
catalytic |
| sequence |
C
|
| description |
375
|
| source |
MCSA : MCSA2
|
|
|
11)
|
chain |
B |
| residue |
205 |
| type |
catalytic |
| sequence |
H
|
| description |
375
|
| source |
MCSA : MCSA2
|
|
|
12)
|
chain |
B |
| residue |
218 |
| type |
catalytic |
| sequence |
Q
|
| description |
375
|
| source |
MCSA : MCSA2
|
|
|
13)
|
chain |
B |
| residue |
5 |
| type |
BINDING |
| sequence |
I
|
| description |
BINDING => ECO:0000305|PubMed:30337572, ECO:0007744|PDB:4TS3, ECO:0007744|PDB:4TS9, ECO:0007744|PDB:4TTA, ECO:0007744|PDB:4TTI, ECO:0007744|PDB:4TTJ
|
| source |
Swiss-Prot : SWS_FT_FI2
|
|
|
14)
|
chain |
B |
| residue |
21 |
| type |
BINDING |
| sequence |
D
|
| description |
in other chain => ECO:0000269|PubMed:11786017, ECO:0000269|PubMed:21672603, ECO:0000269|PubMed:30337572, ECO:0007744|PDB:1K9S, ECO:0007744|PDB:3ONV, ECO:0007744|PDB:3OOE, ECO:0007744|PDB:3OOH, ECO:0007744|PDB:3OPV, ECO:0007744|PDB:4TS3, ECO:0007744|PDB:4TS9, ECO:0007744|PDB:4TTA, ECO:0007744|PDB:4TTI, ECO:0007744|PDB:4TTJ
|
| source |
Swiss-Prot : SWS_FT_FI3
|
|
|
15)
|
chain |
B |
| residue |
25 |
| type |
BINDING |
| sequence |
A
|
| description |
in other chain => ECO:0000269|PubMed:11786017, ECO:0000269|PubMed:21672603, ECO:0000269|PubMed:30337572, ECO:0007744|PDB:1K9S, ECO:0007744|PDB:3ONV, ECO:0007744|PDB:3OOE, ECO:0007744|PDB:3OOH, ECO:0007744|PDB:3OPV, ECO:0007744|PDB:4TS3, ECO:0007744|PDB:4TS9, ECO:0007744|PDB:4TTA, ECO:0007744|PDB:4TTI, ECO:0007744|PDB:4TTJ
|
| source |
Swiss-Prot : SWS_FT_FI3
|
|
|
16)
|
chain |
B |
| residue |
88 |
| type |
BINDING |
| sequence |
V
|
| description |
in other chain => ECO:0000269|PubMed:11786017, ECO:0000269|PubMed:21672603, ECO:0000269|PubMed:30337572, ECO:0007744|PDB:1K9S, ECO:0007744|PDB:3ONV, ECO:0007744|PDB:3OOE, ECO:0007744|PDB:3OOH, ECO:0007744|PDB:3OPV, ECO:0007744|PDB:4TS3, ECO:0007744|PDB:4TS9, ECO:0007744|PDB:4TTA, ECO:0007744|PDB:4TTI, ECO:0007744|PDB:4TTJ
|
| source |
Swiss-Prot : SWS_FT_FI3
|
|
|
17)
|
chain |
B |
| residue |
44 |
| type |
BINDING |
| sequence |
G
|
| description |
BINDING => ECO:0000269|PubMed:11786017, ECO:0000269|PubMed:21672603, ECO:0000269|PubMed:30337572, ECO:0007744|PDB:1K9S, ECO:0007744|PDB:3ONV, ECO:0007744|PDB:3OOE, ECO:0007744|PDB:3OOH, ECO:0007744|PDB:3OPV, ECO:0007744|PDB:4TS3, ECO:0007744|PDB:4TS9, ECO:0007744|PDB:4TTA, ECO:0007744|PDB:4TTI, ECO:0007744|PDB:4TTJ
|
| source |
Swiss-Prot : SWS_FT_FI4
|
|
|
18)
|
chain |
B |
| residue |
180 |
| type |
BINDING |
| sequence |
M
|
| description |
in other chain => ECO:0000305|PubMed:30337572, ECO:0007744|PDB:4TS3, ECO:0007744|PDB:4TS9, ECO:0007744|PDB:4TTA, ECO:0007744|PDB:4TTI, ECO:0007744|PDB:4TTJ
|
| source |
Swiss-Prot : SWS_FT_FI5
|
|
|
19)
|
chain |
B |
| residue |
204 |
| type |
BINDING |
| sequence |
D
|
| description |
in other chain => ECO:0000305|PubMed:30337572, ECO:0007744|PDB:4TS3, ECO:0007744|PDB:4TS9, ECO:0007744|PDB:4TTA, ECO:0007744|PDB:4TTI, ECO:0007744|PDB:4TTJ
|
| source |
Swiss-Prot : SWS_FT_FI5
|
|
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20)
|
chain |
B |
| residue |
218 |
| type |
SITE |
| sequence |
Q
|
| description |
Important for catalytic activity => ECO:0000255|HAMAP-Rule:MF_01627, ECO:0000269|PubMed:21672603, ECO:0000269|PubMed:30337572
|
| source |
Swiss-Prot : SWS_FT_FI6
|
|
|
21)
|
chain |
B |
| residue |
27 |
| type |
MOD_RES |
| sequence |
Y
|
| description |
N6-acetyllysine => ECO:0000269|PubMed:18723842
|
| source |
Swiss-Prot : SWS_FT_FI7
|
|
|
22)
|
chain |
B |
| residue |
205 |
| type |
ACT_SITE |
| sequence |
H
|
| description |
Proton donor => ECO:0000255|HAMAP-Rule:MF_01627, ECO:0000269|PubMed:30337572
|
| source |
Swiss-Prot : SWS_FT_FI1
|
|
|
|