eF-site ID 1nhq-A PDB Code 1nhq Chain A click to enlarge Title CRYSTALLOGRAPHIC ANALYSES OF NADH PEROXIDASE CYS42ALA AND CYS42SER MUTANTS: ACTIVE SITE STRUCTURE, MECHANISTIC IMPLICATIONS, AND AN UNUSUAL ENVIRONMENT OF ARG303 Classification OXIDOREDUCTASE (H2O2(A)) Compound NADH PEROXIDASE Source Enterococcus faecalis (Streptococcus faecalis) (NAPE_ENTFA) Sequence A:  MKVIVLGSSHGGYEAVEELLNLHPDAEIQWYEKGDFISFL SSGMQLYLEGKVKDVNSVRYMTGEKMESRGVNVFSNTEIT AIQPKEHQVTVKDLVSGEERVENYDKLIISPGAVPFELDI PGKDLDNIYLMRGRQWAIKLKQKTVDPEVNNVVVIGSGYI GIEAAEAFAKAGKKVTVIDILDRPLGVYLDKEFTDVLTEE MEANNITIATGETVERYEGDGRVQKVVTDKNAYDADLVVV AVGVRPNTAWLKGTLELHPNGLIKTDEYMRTSEPDVFAVG DATLIKYNPADTEVNIALATNARKQGRFAVKNLEEPVKPF PGVQGSSGLAVFDYKFASTGINEVMAQKLGKETKAVTVVE DYLMDFNPDKQKAWFKLVYDPETTQILGAQLMSKADLTAN INAISLAIQAKMTIEDLAYADFFFQPAFDKPWNIINTAAL EAVKQER Description Functional site 1) chain A residue 158 type sequence G description BINDING SITE FOR RESIDUE SO4 A 449 source : AC1 2) chain A residue 159 type sequence Y description BINDING SITE FOR RESIDUE SO4 A 449 source : AC1 3) chain A residue 160 type sequence I description BINDING SITE FOR RESIDUE SO4 A 449 source : AC1 4) chain A residue 188 type sequence Y description BINDING SITE FOR RESIDUE SO4 A 449 source : AC1 5) chain A residue 6 type sequence L description BINDING SITE FOR RESIDUE FAD A 448 source : AC2 6) chain A residue 7 type sequence G description BINDING SITE FOR RESIDUE FAD A 448 source : AC2 7) chain A residue 9 type sequence S description BINDING SITE FOR RESIDUE FAD A 448 source : AC2 8) chain A residue 10 type sequence H description BINDING SITE FOR RESIDUE FAD A 448 source : AC2 9) chain A residue 11 type sequence G description BINDING SITE FOR RESIDUE FAD A 448 source : AC2 10) chain A residue 32 type sequence E description BINDING SITE FOR RESIDUE FAD A 448 source : AC2 11) chain A residue 33 type sequence K description BINDING SITE FOR RESIDUE FAD A 448 source : AC2 12) chain A residue 41 type sequence S description BINDING SITE FOR RESIDUE FAD A 448 source : AC2 13) chain A residue 42 type sequence S description BINDING SITE FOR RESIDUE FAD A 448 source : AC2 14) chain A residue 79 type sequence I description BINDING SITE FOR RESIDUE FAD A 448 source : AC2 15) chain A residue 110 type sequence S description BINDING SITE FOR RESIDUE FAD A 448 source : AC2 16) chain A residue 111 type sequence P description BINDING SITE FOR RESIDUE FAD A 448 source : AC2 17) chain A residue 112 type sequence G description BINDING SITE FOR RESIDUE FAD A 448 source : AC2 18) chain A residue 113 type sequence A description BINDING SITE FOR RESIDUE FAD A 448 source : AC2 19) chain A residue 131 type sequence M description BINDING SITE FOR RESIDUE FAD A 448 source : AC2 20) chain A residue 132 type sequence R description BINDING SITE FOR RESIDUE FAD A 448 source : AC2 21) chain A residue 159 type sequence Y description BINDING SITE FOR RESIDUE FAD A 448 source : AC2 22) chain A residue 247 type sequence N description BINDING SITE FOR RESIDUE FAD A 448 source : AC2 23) chain A residue 280 type sequence G description BINDING SITE FOR RESIDUE FAD A 448 source : AC2 24) chain A residue 281 type sequence D description BINDING SITE FOR RESIDUE FAD A 448 source : AC2 25) chain A residue 297 type sequence A description BINDING SITE FOR RESIDUE FAD A 448 source : AC2 26) chain A residue 298 type sequence L description BINDING SITE FOR RESIDUE FAD A 448 source : AC2 27) chain A residue 299 type sequence A description BINDING SITE FOR RESIDUE FAD A 448 source : AC2 28) chain A residue 424 type sequence F description BINDING SITE FOR RESIDUE FAD A 448 source : AC2 29) chain A residue 425 type sequence Q description BINDING SITE FOR RESIDUE FAD A 448 source : AC2 30) chain A residue 42 type ACT_SITE sequence S description Redox-active => ECO:0000269|PubMed:8756456 source Swiss-Prot : SWS_FT_FI2 31) chain A residue 7 type BINDING sequence G description BINDING => ECO:0000269|PubMed:8425532, ECO:0000269|PubMed:8756456 source Swiss-Prot : SWS_FT_FI3 32) chain A residue 32 type BINDING sequence E description BINDING => ECO:0000269|PubMed:8425532, ECO:0000269|PubMed:8756456 source Swiss-Prot : SWS_FT_FI3 33) chain A residue 42 type BINDING sequence S description BINDING => ECO:0000269|PubMed:8425532, ECO:0000269|PubMed:8756456 source Swiss-Prot : SWS_FT_FI3 34) chain A residue 110 type BINDING sequence S description BINDING => ECO:0000269|PubMed:8425532, ECO:0000269|PubMed:8756456 source Swiss-Prot : SWS_FT_FI3 35) chain A residue 132 type BINDING sequence R description BINDING => ECO:0000269|PubMed:8425532, ECO:0000269|PubMed:8756456 source Swiss-Prot : SWS_FT_FI3 36) chain A residue 281 type BINDING sequence D description BINDING => ECO:0000269|PubMed:8425532, ECO:0000269|PubMed:8756456 source Swiss-Prot : SWS_FT_FI3 37) chain A residue 299 type BINDING sequence A description BINDING => ECO:0000269|PubMed:8425532, ECO:0000269|PubMed:8756456 source Swiss-Prot : SWS_FT_FI3 38) chain A residue 42 type MOD_RES sequence S description Cysteine sulfenic acid (-SOH) => ECO:0000269|PubMed:8756456 source Swiss-Prot : SWS_FT_FI5 39) chain A residue 10 type catalytic sequence H description 895 source MCSA : MCSA1 40) chain A residue 41 type catalytic sequence S description 895 source MCSA : MCSA1 41) chain A residue 42 type catalytic sequence S description 895 source MCSA : MCSA1 42) chain A residue 303 type catalytic sequence R description 895 source MCSA : MCSA1 43) chain A residue 10 type ACT_SITE sequence H description Proton acceptor => ECO:0000269|PubMed:8756456 source Swiss-Prot : SWS_FT_FI1 44) chain A residue 160 type BINDING sequence I description BINDING => ECO:0000269|PubMed:8425532 source Swiss-Prot : SWS_FT_FI4 45) chain A residue 179 type BINDING sequence D description BINDING => ECO:0000269|PubMed:8425532 source Swiss-Prot : SWS_FT_FI4 46) chain A residue 188 type BINDING sequence Y description BINDING => ECO:0000269|PubMed:8425532 source Swiss-Prot : SWS_FT_FI4 47) chain A residue 243 type BINDING sequence G description BINDING => ECO:0000269|PubMed:8425532 source Swiss-Prot : SWS_FT_FI4 48) chain A residue 297 type BINDING sequence A description BINDING => ECO:0000269|PubMed:8425532 source Swiss-Prot : SWS_FT_FI4 49) chain A residue 328 type BINDING sequence G description BINDING => ECO:0000269|PubMed:8425532 source Swiss-Prot : SWS_FT_FI4

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