eF-site ID 1ldc-B
PDB Code 1ldc
Chain B

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Title X-RAY STRUCTURE OF TWO COMPLEXES OF THE Y143F FLAVOCYTOCHROME B2 MUTANT CRYSTALLIZED IN THE PRESENCE OF LACTATE OR PHENYL-LACTATE
Classification OXIDOREDUCTASE
Compound L-LACTATE DEHYDROGENASE
Source Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (CYB2_YEAST)
Sequence B:  TKEDIARKEQLKSLLPPLDNIINLYDFEYLASQTLTKQAW
AFYSSGANDEVTHRENHNAYHRIFFKPKILVDVRKVDIST
DMLGSHVDVPFYVSATALCKLGNPLEGEKDVARGCGQGVT
KVPQMISTLASCSPEEIIEAAPSDKQIQWYQLYVNSDRKI
TDDLVKNVEKLGVKALFVTVDAPSLGQREKDMKLKFIDPS
LTWKDIEELKKKTKLPIVIKGVQRTEDVIKAAEIGVSGVV
LSNHGGRQLDFSRAPIEVLAETMPILEQRNLKDKLEVFVD
GGVRRGTDVLKALCLGAKGVGLGRPFLYANSCYGRNGVEK
AIEILRDEIEMSMRLLGVTSIAELKPDLLDLSTLKARTVG
VPNDVLYNEVYEGPTLTEFEDA
Description (1)  L-LACTATE DEHYDROGENASE: CYTOCHROME C OXIDOREDUCTASE (FLAVOCYTOCHROME B2) (E.C.1.1.2.3) MUTANT WITH TYR 143 REPLACED BY PHE (Y143F) COMPLEXED WITH PYRUVATE


Functional site

1) chain B
residue 143
type
ligand
sequence F
description BINDING SITE FOR RESIDUE FMN B 570
source : AC4

2) chain B
residue 144
type
ligand
sequence Y
description BINDING SITE FOR RESIDUE FMN B 570
source : AC4

3) chain B
residue 195
type
ligand
sequence S
description BINDING SITE FOR RESIDUE FMN B 570
source : AC4

4) chain B
residue 196
type
ligand
sequence A
description BINDING SITE FOR RESIDUE FMN B 570
source : AC4

5) chain B
residue 197
type
ligand
sequence T
description BINDING SITE FOR RESIDUE FMN B 570
source : AC4

6) chain B
residue 198
type
ligand
sequence A
description BINDING SITE FOR RESIDUE FMN B 570
source : AC4

7) chain B
residue 228
type
ligand
sequence S
description BINDING SITE FOR RESIDUE FMN B 570
source : AC4

8) chain B
residue 252
type
ligand
sequence Q
description BINDING SITE FOR RESIDUE FMN B 570
source : AC4

9) chain B
residue 254
type
ligand
sequence Y
description BINDING SITE FOR RESIDUE FMN B 570
source : AC4

10) chain B
residue 280
type
ligand
sequence T
description BINDING SITE FOR RESIDUE FMN B 570
source : AC4

11) chain B
residue 349
type
ligand
sequence K
description BINDING SITE FOR RESIDUE FMN B 570
source : AC4

12) chain B
residue 373
type
ligand
sequence H
description BINDING SITE FOR RESIDUE FMN B 570
source : AC4

13) chain B
residue 374
type
ligand
sequence G
description BINDING SITE FOR RESIDUE FMN B 570
source : AC4

14) chain B
residue 376
type
ligand
sequence R
description BINDING SITE FOR RESIDUE FMN B 570
source : AC4

15) chain B
residue 409
type
ligand
sequence D
description BINDING SITE FOR RESIDUE FMN B 570
source : AC4

16) chain B
residue 410
type
ligand
sequence G
description BINDING SITE FOR RESIDUE FMN B 570
source : AC4

17) chain B
residue 411
type
ligand
sequence G
description BINDING SITE FOR RESIDUE FMN B 570
source : AC4

18) chain B
residue 413
type
ligand
sequence R
description BINDING SITE FOR RESIDUE FMN B 570
source : AC4

19) chain B
residue 432
type
ligand
sequence G
description BINDING SITE FOR RESIDUE FMN B 570
source : AC4

20) chain B
residue 433
type
ligand
sequence R
description BINDING SITE FOR RESIDUE FMN B 570
source : AC4

21) chain B
residue 436
type
ligand
sequence L
description BINDING SITE FOR RESIDUE FMN B 570
source : AC4

22) chain B
residue 143
type
ligand
sequence F
description BINDING SITE FOR RESIDUE PYR B 580
source : AC5

23) chain B
residue 198
type
ligand
sequence A
description BINDING SITE FOR RESIDUE PYR B 580
source : AC5

24) chain B
residue 254
type
ligand
sequence Y
description BINDING SITE FOR RESIDUE PYR B 580
source : AC5

25) chain B
residue 289
type
ligand
sequence R
description BINDING SITE FOR RESIDUE PYR B 580
source : AC5

26) chain B
residue 373
type
ligand
sequence H
description BINDING SITE FOR RESIDUE PYR B 580
source : AC5

27) chain B
residue 376
type
ligand
sequence R
description BINDING SITE FOR RESIDUE PYR B 580
source : AC5

28) chain B
residue 373
type ACT_SITE
ligand
sequence H
description Proton acceptor.
source Swiss-Prot : SWS_FT_FI4

29) chain B
residue 376
type BINDING
ligand
sequence R
description Substrate.
source Swiss-Prot : SWS_FT_FI6

30) chain B
residue 371-377
type prosite
ligand
sequence SNHGGRQ
description FMN-dependent alpha-hydroxy acid dehydrogenases active site.S-N-H-G-[AG]-R-Q.
source prosite : PS00557

31) chain B
residue 254
type catalytic
ligand
sequence Y
description Annotated By Reference To The Literature 1fcb
source CSA : CSA2

32) chain B
residue 143
type catalytic
ligand
sequence F
description Annotated By Reference To The Literature 1fcb
source CSA : CSA2

33) chain B
residue 376
type catalytic
ligand
sequence R
description Annotated By Reference To The Literature 1fcb
source CSA : CSA2

34) chain B
residue 282
type catalytic
ligand
sequence D
description Annotated By Reference To The Literature 1fcb
source CSA : CSA2

35) chain B
residue 373
type catalytic
ligand
sequence H
description Annotated By Reference To The Literature 1fcb
source CSA : CSA2

36) chain B
residue 254
type catalytic
ligand
sequence Y
description Annotated By Reference To The Literature 1fcb
source CSA : CSA4

37) chain B
residue 376
type catalytic
ligand
sequence R
description Annotated By Reference To The Literature 1fcb
source CSA : CSA4

38) chain B
residue 373
type catalytic
ligand
sequence H
description Annotated By Reference To The Literature 1fcb
source CSA : CSA4

39) chain B
residue 282
type catalytic
ligand
sequence D
description Annotated By Reference To The Literature 1fcb
source CSA : CSA4


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