eF-site ID 1ldc-AB PDB Code 1ldc Chain A, B click to enlarge Title X-RAY STRUCTURE OF TWO COMPLEXES OF THE Y143F FLAVOCYTOCHROME B2 MUTANT CRYSTALLIZED IN THE PRESENCE OF LACTATE OR PHENYL-LACTATE Classification OXIDOREDUCTASE Compound L-LACTATE DEHYDROGENASE Source null (CYB2_YEAST) Sequence A:  KISPAEVAKHNKPDDCWVVINGYVYDLTRFLPNHPGGQDV IKFNAGKDVTAIFEPLHAPNVIDKYIAPEKKLGPLQGSMP PELVCPPYAPGETKEDIARKEQLKSLLPPLDNIINLYDFE YLASQTLTKQAWAFYSSGANDEVTHRENHNAYHRIFFKPK ILVDVRKVDISTDMLGSHVDVPFYVSATALCKLGNPLEGE KDVARGCGQGVTKVPQMISTLASCSPEEIIEAAPSDKQIQ WYQLYVNSDRKITDDLVKNVEKLGVKALFVTVDAPSLGQR EKDMKLKALSKFIDPSLTWKDIEELKKKTKLPIVIKGVQR TEDVIKAAEIGVSGVVLSNHGGRQLDFSRAPIEVLAETMP ILEQRNLKDKLEVFVDGGVRRGTDVLKALCLGAKGVGLGR PFLYANSCYGRNGVEKAIEILRDEIEMSMRLLGVTSIAEL KPDLLDLSTLKARTVGVPNDVLYNEVYEGPTLTEFEDA B:  TKEDIARKEQLKSLLPPLDNIINLYDFEYLASQTLTKQAW AFYSSGANDEVTHRENHNAYHRIFFKPKILVDVRKVDIST DMLGSHVDVPFYVSATALCKLGNPLEGEKDVARGCGQGVT KVPQMISTLASCSPEEIIEAAPSDKQIQWYQLYVNSDRKI TDDLVKNVEKLGVKALFVTVDAPSLGQREKDMKLKFIDPS LTWKDIEELKKKTKLPIVIKGVQRTEDVIKAAEIGVSGVV LSNHGGRQLDFSRAPIEVLAETMPILEQRNLKDKLEVFVD GGVRRGTDVLKALCLGAKGVGLGRPFLYANSCYGRNGVEK AIEILRDEIEMSMRLLGVTSIAELKPDLLDLSTLKARTVG VPNDVLYNEVYEGPTLTEFEDA Description Functional site 1) chain A residue 144 type sequence Y description BINDING SITE FOR RESIDUE FMN A 570 source : AC1 2) chain A residue 195 type sequence S description BINDING SITE FOR RESIDUE FMN A 570 source : AC1 3) chain A residue 196 type sequence A description BINDING SITE FOR RESIDUE FMN A 570 source : AC1 4) chain A residue 197 type sequence T description BINDING SITE FOR RESIDUE FMN A 570 source : AC1 5) chain A residue 198 type sequence A description BINDING SITE FOR RESIDUE FMN A 570 source : AC1 6) chain A residue 228 type sequence S description BINDING SITE FOR RESIDUE FMN A 570 source : AC1 7) chain A residue 252 type sequence Q description BINDING SITE FOR RESIDUE FMN A 570 source : AC1 8) chain A residue 280 type sequence T description BINDING SITE FOR RESIDUE FMN A 570 source : AC1 9) chain A residue 349 type sequence K description BINDING SITE FOR RESIDUE FMN A 570 source : AC1 10) chain A residue 371 type sequence S description BINDING SITE FOR RESIDUE FMN A 570 source : AC1 11) chain A residue 373 type sequence H description BINDING SITE FOR RESIDUE FMN A 570 source : AC1 12) chain A residue 374 type sequence G description BINDING SITE FOR RESIDUE FMN A 570 source : AC1 13) chain A residue 376 type sequence R description BINDING SITE FOR RESIDUE FMN A 570 source : AC1 14) chain A residue 409 type sequence D description BINDING SITE FOR RESIDUE FMN A 570 source : AC1 15) chain A residue 410 type sequence G description BINDING SITE FOR RESIDUE FMN A 570 source : AC1 16) chain A residue 411 type sequence G description BINDING SITE FOR RESIDUE FMN A 570 source : AC1 17) chain A residue 413 type sequence R description BINDING SITE FOR RESIDUE FMN A 570 source : AC1 18) chain A residue 432 type sequence G description BINDING SITE FOR RESIDUE FMN A 570 source : AC1 19) chain A residue 433 type sequence R description BINDING SITE FOR RESIDUE FMN A 570 source : AC1 20) chain A residue 36 type sequence L description BINDING SITE FOR RESIDUE HEM A 560 source : AC2 21) chain A residue 39 type sequence F description BINDING SITE FOR RESIDUE HEM A 560 source : AC2 22) chain A residue 43 type sequence H description BINDING SITE FOR RESIDUE HEM A 560 source : AC2 23) chain A residue 44 type sequence P description BINDING SITE FOR RESIDUE HEM A 560 source : AC2 24) chain A residue 49 type sequence V description BINDING SITE FOR RESIDUE HEM A 560 source : AC2 25) chain A residue 62 type sequence F description BINDING SITE FOR RESIDUE HEM A 560 source : AC2 26) chain A residue 66 type sequence H description BINDING SITE FOR RESIDUE HEM A 560 source : AC2 27) chain A residue 70 type sequence V description BINDING SITE FOR RESIDUE HEM A 560 source : AC2 28) chain A residue 71 type sequence I description BINDING SITE FOR RESIDUE HEM A 560 source : AC2 29) chain A residue 75 type sequence I description BINDING SITE FOR RESIDUE HEM A 560 source : AC2 30) chain A residue 97 type sequence Y description BINDING SITE FOR RESIDUE HEM A 560 source : AC2 31) chain A residue 139 type sequence Q description BINDING SITE FOR RESIDUE HEM A 560 source : AC2 32) chain A residue 199 type sequence L description BINDING SITE FOR RESIDUE HEM A 560 source : AC2 33) chain A residue 230 type sequence L description BINDING SITE FOR RESIDUE HEM A 560 source : AC2 34) chain A residue 296 type sequence K description BINDING SITE FOR RESIDUE HEM A 560 source : AC2 35) chain A residue 143 type sequence F description BINDING SITE FOR RESIDUE PYR A 580 source : AC3 36) chain A residue 254 type sequence Y description BINDING SITE FOR RESIDUE PYR A 580 source : AC3 37) chain A residue 289 type sequence R description BINDING SITE FOR RESIDUE PYR A 580 source : AC3 38) chain A residue 373 type sequence H description BINDING SITE FOR RESIDUE PYR A 580 source : AC3 39) chain A residue 376 type sequence R description BINDING SITE FOR RESIDUE PYR A 580 source : AC3 40) chain B residue 143 type sequence F description BINDING SITE FOR RESIDUE FMN B 570 source : AC4 41) chain B residue 144 type sequence Y description BINDING SITE FOR RESIDUE FMN B 570 source : AC4 42) chain B residue 195 type sequence S description BINDING SITE FOR RESIDUE FMN B 570 source : AC4 43) chain B residue 196 type sequence A description BINDING SITE FOR RESIDUE FMN B 570 source : AC4 44) chain B residue 197 type sequence T description BINDING SITE FOR RESIDUE FMN B 570 source : AC4 45) chain B residue 198 type sequence A description BINDING SITE FOR RESIDUE FMN B 570 source : AC4 46) chain B residue 228 type sequence S description BINDING SITE FOR RESIDUE FMN B 570 source : AC4 47) chain B residue 252 type sequence Q description BINDING SITE FOR RESIDUE FMN B 570 source : AC4 48) chain B residue 254 type sequence Y description BINDING SITE FOR RESIDUE FMN B 570 source : AC4 49) chain B residue 280 type sequence T description BINDING SITE FOR RESIDUE FMN B 570 source : AC4 50) chain B residue 349 type sequence K description BINDING SITE FOR RESIDUE FMN B 570 source : AC4 51) chain B residue 373 type sequence H description BINDING SITE FOR RESIDUE FMN B 570 source : AC4 52) chain B residue 374 type sequence G description BINDING SITE FOR RESIDUE FMN B 570 source : AC4 53) chain B residue 376 type sequence R description BINDING SITE FOR RESIDUE FMN B 570 source : AC4 54) chain B residue 409 type sequence D description BINDING SITE FOR RESIDUE FMN B 570 source : AC4 55) chain B residue 410 type sequence G description BINDING SITE FOR RESIDUE FMN B 570 source : AC4 56) chain B residue 411 type sequence G description BINDING SITE FOR RESIDUE FMN B 570 source : AC4 57) chain B residue 413 type sequence R description BINDING SITE FOR RESIDUE FMN B 570 source : AC4 58) chain B residue 432 type sequence G description BINDING SITE FOR RESIDUE FMN B 570 source : AC4 59) chain B residue 433 type sequence R description BINDING SITE FOR RESIDUE FMN B 570 source : AC4 60) chain B residue 436 type sequence L description BINDING SITE FOR RESIDUE FMN B 570 source : AC4 61) chain B residue 143 type sequence F description BINDING SITE FOR RESIDUE PYR B 580 source : AC5 62) chain B residue 198 type sequence A description BINDING SITE FOR RESIDUE PYR B 580 source : AC5 63) chain B residue 254 type sequence Y description BINDING SITE FOR RESIDUE PYR B 580 source : AC5 64) chain B residue 289 type sequence R description BINDING SITE FOR RESIDUE PYR B 580 source : AC5 65) chain B residue 373 type sequence H description BINDING SITE FOR RESIDUE PYR B 580 source : AC5 66) chain B residue 376 type sequence R description BINDING SITE FOR RESIDUE PYR B 580 source : AC5 67) chain A residue 254 type catalytic sequence Y description 102 source MCSA : MCSA1 68) chain A residue 282 type catalytic sequence D description 102 source MCSA : MCSA1 69) chain A residue 373 type catalytic sequence H description 102 source MCSA : MCSA1 70) chain B residue 254 type catalytic sequence Y description 102 source MCSA : MCSA2 71) chain B residue 282 type catalytic sequence D description 102 source MCSA : MCSA2 72) chain B residue 373 type catalytic sequence H description 102 source MCSA : MCSA2 73) chain A residue 39-46 type prosite sequence FLPNHPGG description CYTOCHROME_B5_1 Cytochrome b5 family, heme-binding domain signature. FLPNHPGG source prosite : PS00191 74) chain A residue 371-377 type prosite sequence SNHGGRQ description FMN_HYDROXY_ACID_DH_1 FMN-dependent alpha-hydroxy acid dehydrogenases active site. SNHGGRQ source prosite : PS00557 75) chain A residue 373 type ACT_SITE sequence H description Proton acceptor => ECO:0000305|PubMed:17563122 source Swiss-Prot : SWS_FT_FI1 76) chain B residue 373 type ACT_SITE sequence H description Proton acceptor => ECO:0000305|PubMed:17563122 source Swiss-Prot : SWS_FT_FI1 77) chain A residue 43 type BINDING sequence H description axial binding residue => ECO:0000269|PubMed:11914072, ECO:0000269|PubMed:2329585, ECO:0007744|PDB:1FCB, ECO:0007744|PDB:1KBI source Swiss-Prot : SWS_FT_FI2 78) chain A residue 66 type BINDING sequence H description axial binding residue => ECO:0000269|PubMed:11914072, ECO:0000269|PubMed:2329585, ECO:0007744|PDB:1FCB, ECO:0007744|PDB:1KBI source Swiss-Prot : SWS_FT_FI2 79) chain A residue 97 type BINDING sequence Y description BINDING => ECO:0000269|PubMed:11914072, ECO:0000269|PubMed:2329585, ECO:0007744|PDB:1FCB, ECO:0007744|PDB:1KBI source Swiss-Prot : SWS_FT_FI3 80) chain A residue 143 type BINDING sequence F description BINDING => ECO:0000269|PubMed:11914072, ECO:0000269|PubMed:2329585, ECO:0007744|PDB:1FCB, ECO:0007744|PDB:1KBI source Swiss-Prot : SWS_FT_FI3 81) chain A residue 376 type BINDING sequence R description BINDING => ECO:0000269|PubMed:11914072, ECO:0000269|PubMed:2329585, ECO:0007744|PDB:1FCB, ECO:0007744|PDB:1KBI source Swiss-Prot : SWS_FT_FI3 82) chain A residue 409 type BINDING sequence D description BINDING => ECO:0000269|PubMed:11914072, ECO:0000269|PubMed:2329585, ECO:0007744|PDB:1FCB, ECO:0007744|PDB:1KBI source Swiss-Prot : SWS_FT_FI3 83) chain A residue 432 type BINDING sequence G description BINDING => ECO:0000269|PubMed:11914072, ECO:0000269|PubMed:2329585, ECO:0007744|PDB:1FCB, ECO:0007744|PDB:1KBI source Swiss-Prot : SWS_FT_FI3 84) chain B residue 143 type BINDING sequence F description BINDING => ECO:0000269|PubMed:11914072, ECO:0000269|PubMed:2329585, ECO:0007744|PDB:1FCB, ECO:0007744|PDB:1KBI source Swiss-Prot : SWS_FT_FI3 85) chain B residue 195 type BINDING sequence S description BINDING => ECO:0000269|PubMed:11914072, ECO:0000269|PubMed:2329585, ECO:0007744|PDB:1FCB, ECO:0007744|PDB:1KBI source Swiss-Prot : SWS_FT_FI3 86) chain B residue 228 type BINDING sequence S description BINDING => ECO:0000269|PubMed:11914072, ECO:0000269|PubMed:2329585, ECO:0007744|PDB:1FCB, ECO:0007744|PDB:1KBI source Swiss-Prot : SWS_FT_FI3 87) chain B residue 252 type BINDING sequence Q description BINDING => ECO:0000269|PubMed:11914072, ECO:0000269|PubMed:2329585, ECO:0007744|PDB:1FCB, ECO:0007744|PDB:1KBI source Swiss-Prot : SWS_FT_FI3 88) chain B residue 254 type BINDING sequence Y description BINDING => ECO:0000269|PubMed:11914072, ECO:0000269|PubMed:2329585, ECO:0007744|PDB:1FCB, ECO:0007744|PDB:1KBI source Swiss-Prot : SWS_FT_FI3 89) chain B residue 280 type BINDING sequence T description BINDING => ECO:0000269|PubMed:11914072, ECO:0000269|PubMed:2329585, ECO:0007744|PDB:1FCB, ECO:0007744|PDB:1KBI source Swiss-Prot : SWS_FT_FI3 90) chain B residue 349 type BINDING sequence K description BINDING => ECO:0000269|PubMed:11914072, ECO:0000269|PubMed:2329585, ECO:0007744|PDB:1FCB, ECO:0007744|PDB:1KBI source Swiss-Prot : SWS_FT_FI3 91) chain B residue 373 type BINDING sequence H description BINDING => ECO:0000269|PubMed:11914072, ECO:0000269|PubMed:2329585, ECO:0007744|PDB:1FCB, ECO:0007744|PDB:1KBI source Swiss-Prot : SWS_FT_FI3 92) chain B residue 376 type BINDING sequence R description BINDING => ECO:0000269|PubMed:11914072, ECO:0000269|PubMed:2329585, ECO:0007744|PDB:1FCB, ECO:0007744|PDB:1KBI source Swiss-Prot : SWS_FT_FI3 93) chain B residue 409 type BINDING sequence D description BINDING => ECO:0000269|PubMed:11914072, ECO:0000269|PubMed:2329585, ECO:0007744|PDB:1FCB, ECO:0007744|PDB:1KBI source Swiss-Prot : SWS_FT_FI3 94) chain B residue 432 type BINDING sequence G description BINDING => ECO:0000269|PubMed:11914072, ECO:0000269|PubMed:2329585, ECO:0007744|PDB:1FCB, ECO:0007744|PDB:1KBI source Swiss-Prot : SWS_FT_FI3 95) chain A residue 252 type BINDING sequence Q description BINDING => ECO:0000269|PubMed:11914072, ECO:0000269|PubMed:2329585, ECO:0007744|PDB:1FCB, ECO:0007744|PDB:1KBI source Swiss-Prot : SWS_FT_FI3 96) chain A residue 254 type BINDING sequence Y description BINDING => ECO:0000269|PubMed:11914072, ECO:0000269|PubMed:2329585, ECO:0007744|PDB:1FCB, ECO:0007744|PDB:1KBI source Swiss-Prot : SWS_FT_FI3 97) chain A residue 280 type BINDING sequence T description BINDING => ECO:0000269|PubMed:11914072, ECO:0000269|PubMed:2329585, ECO:0007744|PDB:1FCB, ECO:0007744|PDB:1KBI source Swiss-Prot : SWS_FT_FI3 98) chain A residue 349 type BINDING sequence K description BINDING => ECO:0000269|PubMed:11914072, ECO:0000269|PubMed:2329585, ECO:0007744|PDB:1FCB, ECO:0007744|PDB:1KBI source Swiss-Prot : SWS_FT_FI3 99) chain A residue 373 type BINDING sequence H description BINDING => ECO:0000269|PubMed:11914072, ECO:0000269|PubMed:2329585, ECO:0007744|PDB:1FCB, ECO:0007744|PDB:1KBI source Swiss-Prot : SWS_FT_FI3 100) chain A residue 195 type BINDING sequence S description BINDING => ECO:0000269|PubMed:11914072, ECO:0000269|PubMed:2329585, ECO:0007744|PDB:1FCB, ECO:0007744|PDB:1KBI source Swiss-Prot : SWS_FT_FI3 101) chain A residue 228 type BINDING sequence S description BINDING => ECO:0000269|PubMed:11914072, ECO:0000269|PubMed:2329585, ECO:0007744|PDB:1FCB, ECO:0007744|PDB:1KBI source Swiss-Prot : SWS_FT_FI3 102) chain A residue 139 type BINDING sequence Q description BINDING => ECO:0000269|PubMed:11914072, ECO:0007744|PDB:1KBI source Swiss-Prot : SWS_FT_FI4 103) chain A residue 296 type BINDING sequence K description BINDING => ECO:0000269|PubMed:11914072, ECO:0007744|PDB:1KBI source Swiss-Prot : SWS_FT_FI4 104) chain B residue 139 type BINDING sequence Q description BINDING => ECO:0000269|PubMed:11914072, ECO:0007744|PDB:1KBI source Swiss-Prot : SWS_FT_FI4 105) chain B residue 296 type BINDING sequence K description BINDING => ECO:0000269|PubMed:11914072, ECO:0007744|PDB:1KBI source Swiss-Prot : SWS_FT_FI4

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