|
|
eF-site ID
|
1jgi-A |
|
PDB Code
|
1jgi |
|
Chain
|
A |
|
click to enlarge
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Title
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Crystal Structure of the Active Site Mutant Glu328Gln of Amylosucrase from Neisseria polysaccharea in Complex with the Natural Substrate Sucrose |
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Classification
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TRANSFERASE |
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Compound
|
amylosucrase |
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Source
|
null (Q9ZEU2_NEIPO) |
|
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Sequence
|
A: |
SPNSQYLKTRILDIYTPEQRAGIEKSEDWRQFSRRMDTHF
PKLMNELDSVYGNNEALLPMLEMLLAQAWQSYSQRNSSLK
DIDIARENNPDWILSNKQVGGVCYVDLFAGDLKGLKDKIP
YFQELGLTYLYLMPLFKCPEGKSDGGYAVSSYRDVNPALG
TIGDLREVIAALHEAGISAVVDFIFNHTSNEHEWAQRCAA
GDPLFDNFYYIFPDRRMPDQYDRTLREIFPDQHPGGFSQL
EDGRWVWTTFNSFQWDLNYSNPWVFRAMAGEMLFLANLGV
DILRMDAVAFIWKQMGTSCENLPQAHALIRAFNAVMRIAA
PAVFFKSQAIVHPDQVVQYIGQDECQIGYNPLQMALLWNT
LATREVNLLHQALTYRHNLPEHTAWVNYVRSHDDIGWTFA
DEDAAYLGISGYDHRQFLNRFFVNRFDGSFARGVPFQYNP
STGDCRVSGTAAALVGLAQDDPHAVDRIKLLYSIALSTGG
LPLIYLGDEVGTLNDDDWSQDSNKSDDSRWAHRPRYNEAL
YAQRNDPSTAAGQIYQDLRHMIAVRQSNPRFDGGRLVTFN
TNNKHIIGYIRNNALLAFGNFSEYPQTVTAHTLQAMPFKA
HDLIGGKTVSLNQDLTLQPYQVMWLEIA
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Description
|
|
Functional site
|
|
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1)
|
chain |
A |
| residue |
286 |
| type |
ACT_SITE |
| sequence |
D
|
| description |
Nucleophile => ECO:0000269|PubMed:15023061, ECO:0000305|PubMed:10828446
|
| source |
Swiss-Prot : SWS_FT_FI1
|
|
|
2)
|
chain |
A |
| residue |
328 |
| type |
ACT_SITE |
| sequence |
Q
|
| description |
Proton donor => ECO:0000305|PubMed:10828446
|
| source |
Swiss-Prot : SWS_FT_FI2
|
|
|
3)
|
chain |
A |
| residue |
144 |
| type |
BINDING |
| sequence |
D
|
| description |
BINDING => ECO:0000305|PubMed:11467966, ECO:0000305|PubMed:12364331, ECO:0000305|PubMed:15023061
|
| source |
Swiss-Prot : SWS_FT_FI3
|
|
|
4)
|
chain |
A |
| residue |
187 |
| type |
BINDING |
| sequence |
H
|
| description |
BINDING => ECO:0000305|PubMed:11467966, ECO:0000305|PubMed:12364331, ECO:0000305|PubMed:15023061
|
| source |
Swiss-Prot : SWS_FT_FI3
|
|
|
5)
|
chain |
A |
| residue |
254 |
| type |
BINDING |
| sequence |
Q
|
| description |
BINDING => ECO:0000305|PubMed:11467966, ECO:0000305|PubMed:12364331, ECO:0000305|PubMed:15023061
|
| source |
Swiss-Prot : SWS_FT_FI3
|
|
|
6)
|
chain |
A |
| residue |
284 |
| type |
BINDING |
| sequence |
R
|
| description |
BINDING => ECO:0000305|PubMed:11467966, ECO:0000305|PubMed:12364331, ECO:0000305|PubMed:15023061
|
| source |
Swiss-Prot : SWS_FT_FI3
|
|
|
7)
|
chain |
A |
| residue |
392 |
| type |
BINDING |
| sequence |
H
|
| description |
BINDING => ECO:0000305|PubMed:11467966, ECO:0000305|PubMed:12364331, ECO:0000305|PubMed:15023061
|
| source |
Swiss-Prot : SWS_FT_FI3
|
|
|
8)
|
chain |
A |
| residue |
393 |
| type |
BINDING |
| sequence |
D
|
| description |
BINDING => ECO:0000305|PubMed:11467966, ECO:0000305|PubMed:12364331, ECO:0000305|PubMed:15023061
|
| source |
Swiss-Prot : SWS_FT_FI3
|
|
|
9)
|
chain |
A |
| residue |
509 |
| type |
BINDING |
| sequence |
R
|
| description |
BINDING => ECO:0000305|PubMed:11467966, ECO:0000305|PubMed:12364331, ECO:0000305|PubMed:15023061
|
| source |
Swiss-Prot : SWS_FT_FI3
|
|
|
10)
|
chain |
A |
| residue |
444 |
| type |
SITE |
| sequence |
D
|
| description |
Transition state stabilizer => ECO:0000250
|
| source |
Swiss-Prot : SWS_FT_FI4
|
|
|
|