eF-site ID 1fcm-AB PDB Code 1fcm Chain A, B click to enlarge Title CRYSTAL STRUCTURE OF THE E.COLI AMPC BETA-LACTAMASE MUTANT Q120L/Y150E COVALENTLY ACYLATED WITH THE INHIBITORY BETA-LACTAM, CLOXACILLIN Classification hydrolase/antibiotic Compound BETA-LACTAMASE Source Escherichia coli (strain K12) (AMPC_ECOLI) Sequence A:  APQQINDIVHRTITPLIEQQKIPGMAVAVIYQGKPYYFTW GYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEI KLSDPTTKYWPELTAKQWNGITLLHLATYTAGGLPLLVPD EVKSSSDLLRFYQNWQPAWAPGTQRLEANSSIGLFGALAV KPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWGY REGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDI NEKTLQQGIQLAQSRYWQTGDMYQGLGWEMLDWPVNPDSI KIALAARPVKAITPPTPAVRASWVHKTGATGGFGSYVAFI PEKELGIVMLANKNYPNPARVDAAWQILNALQ B:  APQQINDIVHRTITPLIEQQKIPGMAVAVIYQGKPYYFTW GYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEI KLSDPTTKYWPELTAKQWNGITLLHLATYTAGGLPLLVPD EVKSSSDLLRFYQNWQPAWAPGTQRLEANSSIGLFGALAV KPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWGY REGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDI NEKTLQQGIQLAQSRYWQTGDMYQGLGWEMLDWPVNPDSI INGSDNKIALAARPVKAITPPTPAVRASWVHKTGATGGFG SYVAFIPEKELGIVMLANKNYPNPARVDAAWQILNALQ Description Functional site 1) chain A residue 61 type sequence S description BINDING SITE FOR RESIDUE CXU A 961 source : AC1 2) chain A residue 116 type sequence L description BINDING SITE FOR RESIDUE CXU A 961 source : AC1 3) chain A residue 117 type sequence L description BINDING SITE FOR RESIDUE CXU A 961 source : AC1 4) chain A residue 147 type sequence E description BINDING SITE FOR RESIDUE CXU A 961 source : AC1 5) chain A residue 149 type sequence N description BINDING SITE FOR RESIDUE CXU A 961 source : AC1 6) chain A residue 218 type sequence Y description BINDING SITE FOR RESIDUE CXU A 961 source : AC1 7) chain A residue 313 type sequence T description BINDING SITE FOR RESIDUE CXU A 961 source : AC1 8) chain A residue 314 type sequence G description BINDING SITE FOR RESIDUE CXU A 961 source : AC1 9) chain A residue 315 type sequence A description BINDING SITE FOR RESIDUE CXU A 961 source : AC1 10) chain A residue 340 type sequence N description BINDING SITE FOR RESIDUE CXU A 961 source : AC1 11) chain A residue 343 type sequence N description BINDING SITE FOR RESIDUE CXU A 961 source : AC1 12) chain B residue 1 type sequence A description BINDING SITE FOR RESIDUE CXU A 961 source : AC1 13) chain B residue 61 type sequence S description BINDING SITE FOR RESIDUE CXU B 961 source : AC2 14) chain B residue 117 type sequence L description BINDING SITE FOR RESIDUE CXU B 961 source : AC2 15) chain B residue 147 type sequence E description BINDING SITE FOR RESIDUE CXU B 961 source : AC2 16) chain B residue 149 type sequence N description BINDING SITE FOR RESIDUE CXU B 961 source : AC2 17) chain B residue 218 type sequence Y description BINDING SITE FOR RESIDUE CXU B 961 source : AC2 18) chain B residue 286 type sequence N description BINDING SITE FOR RESIDUE CXU B 961 source : AC2 19) chain B residue 312 type sequence K description BINDING SITE FOR RESIDUE CXU B 961 source : AC2 20) chain B residue 313 type sequence T description BINDING SITE FOR RESIDUE CXU B 961 source : AC2 21) chain B residue 314 type sequence G description BINDING SITE FOR RESIDUE CXU B 961 source : AC2 22) chain B residue 315 type sequence A description BINDING SITE FOR RESIDUE CXU B 961 source : AC2 23) chain B residue 316 type sequence T description BINDING SITE FOR RESIDUE CXU B 961 source : AC2 24) chain B residue 340 type sequence N description BINDING SITE FOR RESIDUE CXU B 961 source : AC2 25) chain B residue 343 type sequence N description BINDING SITE FOR RESIDUE CXU B 961 source : AC2 26) chain A residue 61 type ACT_SITE sequence S description Acyl-ester intermediate => ECO:0000255|PROSITE-ProRule:PRU10102, ECO:0000269|PubMed:6795623 source Swiss-Prot : SWS_FT_FI1 27) chain B residue 61 type ACT_SITE sequence S description Acyl-ester intermediate => ECO:0000255|PROSITE-ProRule:PRU10102, ECO:0000269|PubMed:6795623 source Swiss-Prot : SWS_FT_FI1 28) chain A residue 147 type ACT_SITE sequence E description Proton acceptor source Swiss-Prot : SWS_FT_FI2 29) chain B residue 147 type ACT_SITE sequence E description Proton acceptor source Swiss-Prot : SWS_FT_FI2 30) chain A residue 312 type BINDING sequence K description BINDING => ECO:0000250 source Swiss-Prot : SWS_FT_FI3 31) chain B residue 312 type BINDING sequence K description BINDING => ECO:0000250 source Swiss-Prot : SWS_FT_FI3 32) chain A residue 57-64 type prosite sequence FELGSVSK description BETA_LACTAMASE_C Beta-lactamase class-C active site. FELGSVSK source prosite : PS00336

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