eF-site ID 1ed8-AB PDB Code 1ed8 Chain A, B click to enlarge Title STRUCTURE OF E. COLI ALKALINE PHOSPHATASE INHIBITED BY THE INORGANIC PHOSPHATE AT 1.75A RESOLUTION Classification HYDROLASE Compound ALKALINE PHOSPHATASE Source Escherichia coli (strain K12) (PPB_ECOLI) Sequence A:  TPEMPVLENRAAQGDITAPGGARRLTGDQTAALRDSLSDK PAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGAL GVDIHEKDHPTILEMAKAAGLATGNVSTAELQDATPAALV AHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARA DVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDA ASLNSVTEANQQKPLLGLFADGNMPVRWLGPKATYHGNID KPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQ VEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEG NTLVIVTADHAHASQIVAPDTKAPGLTQALNTKDGAVMVM SYGNSEEDSQEHTGSQLRIAAYGPHAANVVGLTDQTDLFY TMKAALGLK B:  TPEMPVLENRAAQGDITAPGGARRLTGDQTAALRDSLSDK PAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGAL GVDIHEKDHPTILEMAKAAGLATGNVSTAELQDATPAALV AHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARA DVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDA ASLNSVTEANQQKPLLGLFADGNMPVRWLGPKATYHGNID KPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQ VEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEG NTLVIVTADHAHASQIVAPDTKAPGLTQALNTKDGAVMVM SYGNSEEDSQEHTGSQLRIAAYGPHAANVVGLTDQTDLFY TMKAALGLK Description (1)  ALKALINE PHOSPHATASE (E.C.3.1.3.1) Functional site 1) chain A residue 327 type sequence D description BINDING SITE FOR RESIDUE ZN A 450 source : AC1 2) chain A residue 331 type sequence H description BINDING SITE FOR RESIDUE ZN A 450 source : AC1 3) chain A residue 412 type sequence H description BINDING SITE FOR RESIDUE ZN A 450 source : AC1 4) chain A residue 51 type sequence D description BINDING SITE FOR RESIDUE ZN A 451 source : AC2 5) chain A residue 102 type sequence S description BINDING SITE FOR RESIDUE ZN A 451 source : AC2 6) chain A residue 327 type sequence D description BINDING SITE FOR RESIDUE ZN A 451 source : AC2 7) chain A residue 369 type sequence D description BINDING SITE FOR RESIDUE ZN A 451 source : AC2 8) chain A residue 370 type sequence H description BINDING SITE FOR RESIDUE ZN A 451 source : AC2 9) chain A residue 51 type sequence D description BINDING SITE FOR RESIDUE ZN A 452 source : AC3 10) chain A residue 155 type sequence T description BINDING SITE FOR RESIDUE ZN A 452 source : AC3 11) chain A residue 322 type sequence E description BINDING SITE FOR RESIDUE ZN A 452 source : AC3 12) chain A residue 51 type sequence D description BINDING SITE FOR RESIDUE MG A 462 source : AC4 13) chain A residue 155 type sequence T description BINDING SITE FOR RESIDUE MG A 462 source : AC4 14) chain A residue 322 type sequence E description BINDING SITE FOR RESIDUE MG A 462 source : AC4 15) chain A residue 51 type sequence D description BINDING SITE FOR RESIDUE PO4 A 456 source : AC5 16) chain A residue 102 type sequence S description BINDING SITE FOR RESIDUE PO4 A 456 source : AC5 17) chain A residue 166 type sequence R description BINDING SITE FOR RESIDUE PO4 A 456 source : AC5 18) chain A residue 327 type sequence D description BINDING SITE FOR RESIDUE PO4 A 456 source : AC5 19) chain A residue 331 type sequence H description BINDING SITE FOR RESIDUE PO4 A 456 source : AC5 20) chain A residue 369 type sequence D description BINDING SITE FOR RESIDUE PO4 A 456 source : AC5 21) chain A residue 370 type sequence H description BINDING SITE FOR RESIDUE PO4 A 456 source : AC5 22) chain A residue 412 type sequence H description BINDING SITE FOR RESIDUE PO4 A 456 source : AC5 23) chain A residue 267 type sequence R description BINDING SITE FOR RESIDUE SO4 A 458 source : AC6 24) chain A residue 268 type sequence W description BINDING SITE FOR RESIDUE SO4 A 458 source : AC6 25) chain A residue 292 type sequence R description BINDING SITE FOR RESIDUE SO4 A 458 source : AC6 26) chain B residue 827 type sequence D description BINDING SITE FOR RESIDUE ZN B 950 source : AC7 27) chain B residue 831 type sequence H description BINDING SITE FOR RESIDUE ZN B 950 source : AC7 28) chain B residue 912 type sequence H description BINDING SITE FOR RESIDUE ZN B 950 source : AC7 29) chain B residue 551 type sequence D description BINDING SITE FOR RESIDUE ZN B 951 source : AC8 30) chain B residue 602 type sequence S description BINDING SITE FOR RESIDUE ZN B 951 source : AC8 31) chain B residue 827 type sequence D description BINDING SITE FOR RESIDUE ZN B 951 source : AC8 32) chain B residue 869 type sequence D description BINDING SITE FOR RESIDUE ZN B 951 source : AC8 33) chain B residue 870 type sequence H description BINDING SITE FOR RESIDUE ZN B 951 source : AC8 34) chain B residue 551 type sequence D description BINDING SITE FOR RESIDUE ZN B 952 source : AC9 35) chain B residue 655 type sequence T description BINDING SITE FOR RESIDUE ZN B 952 source : AC9 36) chain B residue 822 type sequence E description BINDING SITE FOR RESIDUE ZN B 952 source : AC9 37) chain B residue 551 type sequence D description BINDING SITE FOR RESIDUE MG B 962 source : BC1 38) chain B residue 655 type sequence T description BINDING SITE FOR RESIDUE MG B 962 source : BC1 39) chain B residue 822 type sequence E description BINDING SITE FOR RESIDUE MG B 962 source : BC1 40) chain B residue 551 type sequence D description BINDING SITE FOR RESIDUE PO4 B 956 source : BC2 41) chain B residue 601 type sequence D description BINDING SITE FOR RESIDUE PO4 B 956 source : BC2 42) chain B residue 602 type sequence S description BINDING SITE FOR RESIDUE PO4 B 956 source : BC2 43) chain B residue 666 type sequence R description BINDING SITE FOR RESIDUE PO4 B 956 source : BC2 44) chain B residue 827 type sequence D description BINDING SITE FOR RESIDUE PO4 B 956 source : BC2 45) chain B residue 831 type sequence H description BINDING SITE FOR RESIDUE PO4 B 956 source : BC2 46) chain B residue 869 type sequence D description BINDING SITE FOR RESIDUE PO4 B 956 source : BC2 47) chain B residue 870 type sequence H description BINDING SITE FOR RESIDUE PO4 B 956 source : BC2 48) chain B residue 912 type sequence H description BINDING SITE FOR RESIDUE PO4 B 956 source : BC2 49) chain B residue 767 type sequence R description BINDING SITE FOR RESIDUE SO4 B 958 source : BC3 50) chain B residue 768 type sequence W description BINDING SITE FOR RESIDUE SO4 B 958 source : BC3 51) chain B residue 792 type sequence R description BINDING SITE FOR RESIDUE SO4 B 958 source : BC3 52) chain A residue 51 type catalytic sequence D description 44 source MCSA : MCSA1 53) chain A residue 369 type catalytic sequence D description 44 source MCSA : MCSA1 54) chain A residue 370 type catalytic sequence H description 44 source MCSA : MCSA1 55) chain A residue 412 type catalytic sequence H description 44 source MCSA : MCSA1 56) chain A residue 102 type catalytic sequence S description 44 source MCSA : MCSA1 57) chain A residue 153 type catalytic sequence D description 44 source MCSA : MCSA1 58) chain A residue 155 type catalytic sequence T description 44 source MCSA : MCSA1 59) chain A residue 166 type catalytic sequence R description 44 source MCSA : MCSA1 60) chain A residue 322 type catalytic sequence E description 44 source MCSA : MCSA1 61) chain A residue 327 type catalytic sequence D description 44 source MCSA : MCSA1 62) chain A residue 328 type catalytic sequence K description 44 source MCSA : MCSA1 63) chain A residue 331 type catalytic sequence H description 44 source MCSA : MCSA1 64) chain B residue 551 type catalytic sequence D description 44 source MCSA : MCSA2 65) chain B residue 869 type catalytic sequence D description 44 source MCSA : MCSA2 66) chain B residue 870 type catalytic sequence H description 44 source MCSA : MCSA2 67) chain B residue 912 type catalytic sequence H description 44 source MCSA : MCSA2 68) chain B residue 602 type catalytic sequence S description 44 source MCSA : MCSA2 69) chain B residue 653 type catalytic sequence D description 44 source MCSA : MCSA2 70) chain B residue 655 type catalytic sequence T description 44 source MCSA : MCSA2 71) chain B residue 666 type catalytic sequence R description 44 source MCSA : MCSA2 72) chain B residue 822 type catalytic sequence E description 44 source MCSA : MCSA2 73) chain B residue 827 type catalytic sequence D description 44 source MCSA : MCSA2 74) chain B residue 828 type catalytic sequence K description 44 source MCSA : MCSA2 75) chain B residue 831 type catalytic sequence H description 44 source MCSA : MCSA2 76) chain A residue 99-107 type prosite sequence VTDSAASAT description ALKALINE_PHOSPHATASE Alkaline phosphatase active site. VtDSAASAT source prosite : PS00123 77) chain A residue 102 type ACT_SITE sequence S description Phosphoserine intermediate source Swiss-Prot : SWS_FT_FI1 78) chain B residue 602 type ACT_SITE sequence S description Phosphoserine intermediate source Swiss-Prot : SWS_FT_FI1 79) chain A residue 51 type BINDING sequence D description source Swiss-Prot : SWS_FT_FI2 80) chain B residue 551 type BINDING sequence D description source Swiss-Prot : SWS_FT_FI2 81) chain B residue 653 type BINDING sequence D description source Swiss-Prot : SWS_FT_FI2 82) chain B residue 655 type BINDING sequence T description source Swiss-Prot : SWS_FT_FI2 83) chain B residue 822 type BINDING sequence E description source Swiss-Prot : SWS_FT_FI2 84) chain B residue 827 type BINDING sequence D description source Swiss-Prot : SWS_FT_FI2 85) chain B residue 831 type BINDING sequence H description source Swiss-Prot : SWS_FT_FI2 86) chain B residue 869 type BINDING sequence D description source Swiss-Prot : SWS_FT_FI2 87) chain B residue 870 type BINDING sequence H description source Swiss-Prot : SWS_FT_FI2 88) chain B residue 912 type BINDING sequence H description source Swiss-Prot : SWS_FT_FI2 89) chain A residue 153 type BINDING sequence D description source Swiss-Prot : SWS_FT_FI2 90) chain A residue 155 type BINDING sequence T description source Swiss-Prot : SWS_FT_FI2 91) chain A residue 322 type BINDING sequence E description source Swiss-Prot : SWS_FT_FI2 92) chain A residue 327 type BINDING sequence D description source Swiss-Prot : SWS_FT_FI2 93) chain A residue 331 type BINDING sequence H description source Swiss-Prot : SWS_FT_FI2 94) chain A residue 369 type BINDING sequence D description source Swiss-Prot : SWS_FT_FI2 95) chain A residue 370 type BINDING sequence H description source Swiss-Prot : SWS_FT_FI2 96) chain A residue 412 type BINDING sequence H description source Swiss-Prot : SWS_FT_FI2

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