|
|
eF-site ID
|
1cyy-AB |
|
PDB Code
|
1cyy |
|
Chain
|
A, B |
|
click to enlarge
|
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Title
|
CRYSTAL STRUCTURE OF THE 30 KDA FRAGMENT OF E. COLI DNA TOPOISOMERASE I. HEXAGONAL FORM |
|
Classification
|
ISOMERASE |
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Compound
|
DNA TOPOISOMERASE I |
|
Source
|
(TOP1_ECOLI) |
|
|
Sequence
|
A: |
EFWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAA
VSLLEKARYSVLEREDKPTTSKPGAPFITSTLQQAASTRL
GFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNMVRG
YISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMA
ESLKDMEADAQKLYQLIWRQFVACQMTPAKYDSTTLTVGA
GDFRLKARGRILRFDGWTKVMPALDRILPAVNKGDALTLV
ELTPAQHFTK
|
| B: |
FVPEEFWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQ
TQAAVSLLEKARYSVLEREDKPTTSKPGAPFITSTLQQAA
STRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVN
MVRGYISDNFGKKYLPESPNQYASEAHEAIRPSDVNVMAE
SLKDMEADAQKLYQLIWRQFVACQMTPAKYDSTTLTVGAG
DFRLKARGRILRFDGWTKVMPALDRILPAVNKGDALTLVE
LTPAQHFTKPP
|
|
|
Description
|
|
Functional site
|
|
|
1)
|
chain |
A |
| residue |
365 |
| type |
|
| sequence |
H
|
| description |
BINDING SITE FOR RESIDUE ZN A 1001
|
| source |
: AC1
|
|
|
2)
|
chain |
A |
| residue |
469 |
| type |
|
| sequence |
H
|
| description |
BINDING SITE FOR RESIDUE ZN A 1001
|
| source |
: AC1
|
|
|
3)
|
chain |
B |
| residue |
463 |
| type |
|
| sequence |
E
|
| description |
BINDING SITE FOR RESIDUE ZN A 1001
|
| source |
: AC1
|
|
|
4)
|
chain |
A |
| residue |
319 |
| type |
catalytic |
| sequence |
Y
|
| description |
366
|
| source |
MCSA : MCSA1
|
|
|
5)
|
chain |
A |
| residue |
321 |
| type |
catalytic |
| sequence |
R
|
| description |
366
|
| source |
MCSA : MCSA1
|
|
|
6)
|
chain |
A |
| residue |
365 |
| type |
catalytic |
| sequence |
H
|
| description |
366
|
| source |
MCSA : MCSA1
|
|
|
7)
|
chain |
B |
| residue |
319 |
| type |
catalytic |
| sequence |
Y
|
| description |
366
|
| source |
MCSA : MCSA2
|
|
|
8)
|
chain |
B |
| residue |
321 |
| type |
catalytic |
| sequence |
R
|
| description |
366
|
| source |
MCSA : MCSA2
|
|
|
9)
|
chain |
B |
| residue |
365 |
| type |
catalytic |
| sequence |
H
|
| description |
366
|
| source |
MCSA : MCSA2
|
|
|
10)
|
chain |
A |
| residue |
309-323 |
| type |
prosite |
| sequence |
QRLYEAGYITYMRTD
|
| description |
TOPO_IA_1 Topoisomerase (Topo) IA-type active site signature. QrLYEagy.........ITYmRTD
|
| source |
prosite : PS00396
|
|
|
11)
|
chain |
A |
| residue |
319 |
| type |
ACT_SITE |
| sequence |
Y
|
| description |
O-(5'-phospho-DNA)-tyrosine intermediate => ECO:0000255|PROSITE-ProRule:PRU01383, ECO:0000269|PubMed:21482796, ECO:0000269|PubMed:8114910, ECO:0000269|PubMed:9497321
|
| source |
Swiss-Prot : SWS_FT_FI1
|
|
|
12)
|
chain |
B |
| residue |
319 |
| type |
ACT_SITE |
| sequence |
Y
|
| description |
O-(5'-phospho-DNA)-tyrosine intermediate => ECO:0000255|PROSITE-ProRule:PRU01383, ECO:0000269|PubMed:21482796, ECO:0000269|PubMed:8114910, ECO:0000269|PubMed:9497321
|
| source |
Swiss-Prot : SWS_FT_FI1
|
|
|
13)
|
chain |
A |
| residue |
321 |
| type |
SITE |
| sequence |
R
|
| description |
Interaction with DNA
|
| source |
Swiss-Prot : SWS_FT_FI2
|
|
|
14)
|
chain |
B |
| residue |
321 |
| type |
SITE |
| sequence |
R
|
| description |
Interaction with DNA
|
| source |
Swiss-Prot : SWS_FT_FI2
|
|
|
|