eF-site ID 1cy1-A
PDB Code 1cy1
Chain A

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Title COMPLEX OF E.COLI DNA TOPOISOMERASE I WITH 5'PTPTPT
Classification ISOMERASE
Compound DNA TOPOISOMERASE I
Source (TOP1_ECOLI)
Sequence A:  GKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPRGA
LVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEKADHI
YLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNA
IRQAFNKPGELNIDRVNAQQARRFMDRVVGYMVSPLLWKK
IARGLSAGRVQSVAVRLVVEREREIKAFVPEEFWEVDAST
TTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARY
SVLEREDKPTTSKPGAPFITSTLQQAASTRLGFGVKKTMM
MAQRLYEAGYITYMRTDSTNLSQDAVNMVRGYISDNFGKK
YLPESPNQYAEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFD
GWTKVMPALILPAVNKGDALTLVELTPAQHFTKPPARFSE
ASLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYA
EKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAE
WKAVLDHFFSDFTQQLDKAEKDPEEGGMRPNQMVLTS
Description


Functional site

1) chain A
residue 202
type
sequence R
description BINDING SITE FOR RESIDUE PO4 A 700
source : AC1

2) chain A
residue 458
type
sequence A
description BINDING SITE FOR RESIDUE PO4 A 700
source : AC1

3) chain A
residue 535
type
sequence R
description BINDING SITE FOR RESIDUE PO4 A 700
source : AC1

4) chain A
residue 539
type
sequence N
description BINDING SITE FOR RESIDUE PO4 A 700
source : AC1

5) chain A
residue 113
type
sequence D
description BINDING SITE FOR RESIDUE TMP A 600
source : AC2

6) chain A
residue 114
type
sequence R
description BINDING SITE FOR RESIDUE TMP A 600
source : AC2

7) chain A
residue 115
type
sequence E
description BINDING SITE FOR RESIDUE TMP A 600
source : AC2

8) chain A
residue 161
type
sequence R
description BINDING SITE FOR RESIDUE TMP A 600
source : AC2

9) chain A
residue 165
type
sequence Q
description BINDING SITE FOR RESIDUE TMP A 600
source : AC2

10) chain A
residue 168
type
sequence R
description BINDING SITE FOR RESIDUE TMP A 600
source : AC2

11) chain A
residue 365
type
sequence H
description BINDING SITE FOR RESIDUE TMP A 600
source : AC2

12) chain A
residue 493
type
sequence R
description BINDING SITE FOR RESIDUE TMP A 600
source : AC2

13) chain A
residue 495
type
sequence S
description BINDING SITE FOR RESIDUE TMP A 600
source : AC2

14) chain A
residue 554
type
sequence A
description BINDING SITE FOR RESIDUE TMP A 600
source : AC2

15) chain A
residue 292
type
sequence A
description BINDING SITE FOR RESIDUE THP A 601
source : AC3

16) chain A
residue 296
type
sequence R
description BINDING SITE FOR RESIDUE THP A 601
source : AC3

17) chain A
residue 393
type
sequence L
description BINDING SITE FOR RESIDUE THP A 601
source : AC3

18) chain A
residue 396
type
sequence R
description BINDING SITE FOR RESIDUE THP A 601
source : AC3

19) chain A
residue 397
type
sequence Q
description BINDING SITE FOR RESIDUE THP A 601
source : AC3

20) chain A
residue 434
type
sequence W
description BINDING SITE FOR RESIDUE THP A 601
source : AC3

21) chain A
residue 438
type
sequence M
description BINDING SITE FOR RESIDUE THP A 601
source : AC3

22) chain A
residue 291
type
sequence Q
description BINDING SITE FOR RESIDUE TMP A 602
source : AC4

23) chain A
residue 294
type
sequence S
description BINDING SITE FOR RESIDUE TMP A 602
source : AC4

24) chain A
residue 295
type
sequence T
description BINDING SITE FOR RESIDUE TMP A 602
source : AC4

25) chain A
residue 478
type
sequence S
description BINDING SITE FOR RESIDUE TMP A 602
source : AC4

26) chain A
residue 481
type
sequence S
description BINDING SITE FOR RESIDUE TMP A 602
source : AC4

27) chain A
residue 516
type
sequence R
description BINDING SITE FOR RESIDUE TMP A 602
source : AC4

28) chain A
residue 9
type catalytic
sequence E
description 366
source MCSA : MCSA1

29) chain A
residue 111
type catalytic
sequence D
description 366
source MCSA : MCSA1

30) chain A
residue 113
type catalytic
sequence D
description 366
source MCSA : MCSA1

31) chain A
residue 115
type catalytic
sequence E
description 366
source MCSA : MCSA1

32) chain A
residue 319
type catalytic
sequence Y
description 366
source MCSA : MCSA1

33) chain A
residue 321
type catalytic
sequence R
description 366
source MCSA : MCSA1

34) chain A
residue 365
type catalytic
sequence H
description 366
source MCSA : MCSA1

35) chain A
residue 309-323
type prosite
sequence QRLYEAGYITYMRTD
description TOPO_IA_1 Topoisomerase (Topo) IA-type active site signature. QrLYEagy.........ITYmRTD
source prosite : PS00396

36) chain A
residue 319
type ACT_SITE
sequence Y
description O-(5'-phospho-DNA)-tyrosine intermediate => ECO:0000255|PROSITE-ProRule:PRU01383, ECO:0000269|PubMed:21482796, ECO:0000269|PubMed:8114910, ECO:0000269|PubMed:9497321
source Swiss-Prot : SWS_FT_FI1

37) chain A
residue 9
type BINDING
sequence E
description BINDING => ECO:0000255|HAMAP-Rule:MF_00952
source Swiss-Prot : SWS_FT_FI2

38) chain A
residue 111
type BINDING
sequence D
description BINDING => ECO:0000305
source Swiss-Prot : SWS_FT_FI3

39) chain A
residue 33
type SITE
sequence H
description Interaction with DNA
source Swiss-Prot : SWS_FT_FI4

40) chain A
residue 168
type SITE
sequence R
description Interaction with DNA
source Swiss-Prot : SWS_FT_FI4

41) chain A
residue 169
type SITE
sequence R
description Interaction with DNA
source Swiss-Prot : SWS_FT_FI4

42) chain A
residue 172
type SITE
sequence D
description Interaction with DNA
source Swiss-Prot : SWS_FT_FI4

43) chain A
residue 177
type SITE
sequence Y
description Interaction with DNA
source Swiss-Prot : SWS_FT_FI4

44) chain A
residue 184
type SITE
sequence W
description Interaction with DNA
source Swiss-Prot : SWS_FT_FI4

45) chain A
residue 321
type SITE
sequence R
description Interaction with DNA
source Swiss-Prot : SWS_FT_FI4

46) chain A
residue 507
type SITE
sequence R
description Interaction with DNA
source Swiss-Prot : SWS_FT_FI4


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