eF-site/Summary 1cjl-A

eF-site ID	1cjl-A
PDB Code	1cjl
Chain	A

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Title	CRYSTAL STRUCTURE OF A CYSTEINE PROTEASE PROFORM
Classification	HYDROLASE
Compound	PROCATHEPSIN L
Source	Homo sapiens (Human) (CATL_HUMAN)

Sequence	A:	DHSLEAQWTKWKAMHNRLYGMNEEGWRRAVWEKNMKMIEL HNQEYREGKHSFTMAMNAFGDMTSEEFRQVMNGLQNRKPR KGKVFQEPLFYEAPRSVDWREKGYVTPVKNQGQCGSSWAF SATGALEGQMFRKTGRLISLSEQNLVDCSGPEGNEGCNGG LMDYAFQYVQDNGGLDSEESYPYEATEESCKYNPKYSVAN DAGFVDIPKQEKALMKAVATVGPISVAIDAGHESFLFYKE GIYFEPDCSSEDMDHGVLVVGYGFESNKYWLVKNSWGEEW GMGGYVKMAKDRRNHCGIASAASYPTV

Description

Functional site


1)	chain	A
	residue	25
	type
	sequence	S
	description	CATALYTIC TRIAD (CYS 25 IS MUTATED TO SER).
	source	: ACT

2)	chain	A
	residue	163
	type
	sequence	H
	description	CATALYTIC TRIAD (CYS 25 IS MUTATED TO SER).
	source	: ACT

3)	chain	A
	residue	187
	type
	sequence	N
	description	CATALYTIC TRIAD (CYS 25 IS MUTATED TO SER).
	source	: ACT

4)	chain	A
	residue	25
	type	ACT_SITE
	sequence	S
	description	ACT_SITE => ECO:0000305\|PubMed:9468501
	source	Swiss-Prot : SWS_FT_FI1

5)	chain	A
	residue	108
	type	CARBOHYD
	sequence	N
	description	N-linked (GlcNAc...) asparagine => ECO:0000269\|PubMed:12754519, ECO:0000269\|PubMed:16335952, ECO:0000269\|PubMed:19159218
	source	Swiss-Prot : SWS_FT_FI4

6)	chain	A
	residue	163
	type	ACT_SITE
	sequence	H
	description
	source	Swiss-Prot : SWS_FT_FI2

7)	chain	A
	residue	187
	type	ACT_SITE
	sequence	N
	description
	source	Swiss-Prot : SWS_FT_FI2

8)	chain	A
	residue	89
	type	SITE
	sequence	Y
	description	Cleavage; by autolysis => ECO:0000269\|PubMed:9468501
	source	Swiss-Prot : SWS_FT_FI3

9)	chain	A
	residue	90
	type	SITE
	sequence	P
	description	Cleavage; by autolysis => ECO:0000269\|PubMed:9468501
	source	Swiss-Prot : SWS_FT_FI3

10)	chain	A
	residue	95
	type	SITE
	sequence	E
	description	Cleavage; by autolysis => ECO:0000269\|PubMed:9468501
	source	Swiss-Prot : SWS_FT_FI3

11)	chain	A
	residue	96
	type	SITE
	sequence	E
	description	Cleavage; by autolysis => ECO:0000269\|PubMed:9468501
	source	Swiss-Prot : SWS_FT_FI3

12)	chain	A
	residue	161-171
	type	prosite
	sequence	MDHGVLVVGYG
	description	THIOL_PROTEASE_HIS Eukaryotic thiol (cysteine) proteases histidine active site. MDHGVLVVGYG
	source	prosite : PS00639

13)	chain	A
	residue	182-201
	type	prosite
	sequence	YWLVKNSWGEEWGMGGYVKM
	description	THIOL_PROTEASE_ASN Eukaryotic thiol (cysteine) proteases asparagine active site. YWLvKNSWgeeWGmgGYVkM
	source	prosite : PS00640