eF-site/Summary 1bjt-A

eF-site ID	1bjt-A
PDB Code	1bjt
Chain	A

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Title	TOPOISOMERASE II RESIDUES 409-1201
Classification	TOPOISOMERASE
Compound	TOPOISOMERASE II
Source	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (TOP2_YEAST)

Sequence	A:	RKSRITNYPKLEDANKAGTKEGYKCTLVLTEGDSALSLAV AGLAVVGRDYYGCYPLRGKMLNVREALKNAEIQAIKKIMG LQHRKKYEDTKSLRYGHLMIMTDSHIKGLIINFLESSFLG LLDIQGFLLEFITPIIKVSITKPTKNTIAFYNMPDYEKWR EEESHKFTWKQKYYKGLGTSLAQEVREYFSNLDRHLKIFH SLQWLRQYEPFINKELILFSLADNIRSIPNVLDGFKPGQR KVLYGCFKKNLKSELKVAQLAPYVSECTAYHHGEQSLAQT IIGLAQNFVGSNNIYLLLPNGAFGTRATGGKDAAAARYIY TELNKLTRKIFHPADDPLYKYIQEDEKTVEPEWYLPILPM ILVNGAEGIGTGWSTYIPPFNPLEIIKNIRHLMNDEELEQ MHPWFRGWTGTIEEIEPLRYRMYGRIEQIGDNVLEITELP ARTWTSTIKEYLLLGLSGNDKIKPWIKDMEEQHDDNIKFI ITLSPEEMAKTRKIGFYERFKLISPISLMNMVAFDPHGKI KKYNSVNEILSEFYYVRLEYYQKRKDHMSERLQWEVEKYS FQVKFIKMIIEKELTVTNKPRNAIIQELENLGFPRFNKEG KPYYGSPEELYGTYEYLLGMRIWSLTKERYQKLLKQKQEK ETELENLLKLSAKDIWNTDLKAFEVGYQEFLQRDAEARG

Description

Functional site


1)	chain	A
	residue	449
	type	BINDING
	sequence	E
	description	BINDING => ECO:0000255\|PROSITE-ProRule:PRU00995, ECO:0000269\|PubMed:18097402
	source	Swiss-Prot : SWS_FT_FI2

2)	chain	A
	residue	526
	type	BINDING
	sequence	D
	description	BINDING => ECO:0000255\|PROSITE-ProRule:PRU00995, ECO:0000269\|PubMed:18097402
	source	Swiss-Prot : SWS_FT_FI2

3)	chain	A
	residue	480
	type	SITE
	sequence	N
	description	Interaction with DNA => ECO:0000269\|PubMed:20485342
	source	Swiss-Prot : SWS_FT_FI4

4)	chain	A
	residue	700
	type	SITE
	sequence	K
	description	Interaction with DNA => ECO:0000269\|PubMed:20485342
	source	Swiss-Prot : SWS_FT_FI4

5)	chain	A
	residue	781
	type	SITE
	sequence	R
	description	Transition state stabilizer => ECO:0000269\|PubMed:20485342
	source	Swiss-Prot : SWS_FT_FI5

6)	chain	A
	residue	833
	type	SITE
	sequence	I
	description	Important for DNA bending; intercalates between base pairs of target DNA => ECO:0000269\|PubMed:18097402
	source	Swiss-Prot : SWS_FT_FI6

7)	chain	A
	residue	447-455
	type	prosite
	sequence	LTEGDSALS
	description	TOPOISOMERASE_II DNA topoisomerase II signature. LTEGDSALS
	source	prosite : PS00177

8)	chain	A
	residue	477
	type	SITE
	sequence	K
	description	Interaction with DNA => ECO:0000255\|PROSITE-ProRule:PRU00995
	source	Swiss-Prot : SWS_FT_FI3

9)	chain	A
	residue	734
	type	SITE
	sequence	Y
	description	Interaction with DNA => ECO:0000255\|PROSITE-ProRule:PRU00995
	source	Swiss-Prot : SWS_FT_FI3

10)	chain	A
	residue	740
	type	SITE
	sequence	S
	description	Interaction with DNA => ECO:0000255\|PROSITE-ProRule:PRU00995
	source	Swiss-Prot : SWS_FT_FI3

11)	chain	A
	residue	908
	type	SITE
	sequence	W
	description	Interaction with DNA => ECO:0000255\|PROSITE-ProRule:PRU00995
	source	Swiss-Prot : SWS_FT_FI3

12)	chain	A
	residue	782
	type	ACT_SITE
	sequence	Y
	description	O-(5'-phospho-DNA)-tyrosine intermediate => ECO:0000255\|PROSITE-ProRule:PRU01384, ECO:0000269\|PubMed:20485342, ECO:0000269\|PubMed:9685374
	source	Swiss-Prot : SWS_FT_FI1