eF-site/Summary 1bgw-A

eF-site ID	1bgw-A
PDB Code	1bgw
Chain	A

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Title	TOPOISOMERASE RESIDUES 410-1202,
Classification	DNA BINDING PROTEIN
Compound	TOPOISOMERASE
Source	null (TOP2_YEAST)

Sequence	A:	RKSRITNYPKLEDANKAGTKEGYKCTLVLTEGDSALSLAV AGLAVVGRDYYGCYPLRGKMLNVREASADQILKNAEIQAI KKIMGLQHRKKYEDTKSLRYGHLMIMTDQDHDGSHIKGLI INFLESSFLGLLDIQGFLLEFITPIIKVSITKPTKNTIAF YNMPDYEKWREEESHKFTWKQKYYKGLGTSLAQEVREYFS NLDRHLKIFHSLQGLFSLADNIRSIPNVLDGFKPGQRKVL YGCFKKNLKSELKVAQLAPYVSECTAYHHGEQSLAQTIIG LAQNFVGSNNIYLLLPNGAFGTRATGGKDAAAARYIYTEL NKLTRKIFHPADDPLYKYIQEDEKTVEPEWYLPILPMILV NGAEGIGTGWSTYIPPFNPLEIIKNIRHLMNDEELEQMHP WFRGWTGTIEEIEPLRYRMYGRIEQIGDNVLEITELPART WTSTIKEYLLLGLSGNDKIKPWIKDMEEQHDDNIKFIITL SPEEMAKTRKIGFYERFKLISPISLMNMVAFDPHGKIKKY NSVNEILSEFYYVRLEYYQKRKDHMSERLQWEVEKYSFQV KFIKMIIEKELTVTNKPRNAIIQELENLGFPRFNKEGKPY YGSPNDEIAEELYGTYEYLLGMRIWSLTKERYQKLLKQKQ EKETELENLLKLSAKDIWNTDLKAFEVGYQEFLQRDAEAR

Description

Functional site


1)	chain	A
	residue	783
	type	ACT_SITE
	sequence	Y
	description	O-(5'-phospho-DNA)-tyrosine intermediate => ECO:0000255\|PROSITE-ProRule:PRU01384, ECO:0000269\|PubMed:20485342, ECO:0000269\|PubMed:9685374
	source	Swiss-Prot : SWS_FT_FI1

2)	chain	A
	residue	478
	type	SITE
	sequence	K
	description	Interaction with DNA => ECO:0000255\|PROSITE-ProRule:PRU00995
	source	Swiss-Prot : SWS_FT_FI3

3)	chain	A
	residue	735
	type	SITE
	sequence	Y
	description	Interaction with DNA => ECO:0000255\|PROSITE-ProRule:PRU00995
	source	Swiss-Prot : SWS_FT_FI3

4)	chain	A
	residue	741
	type	SITE
	sequence	S
	description	Interaction with DNA => ECO:0000255\|PROSITE-ProRule:PRU00995
	source	Swiss-Prot : SWS_FT_FI3

5)	chain	A
	residue	909
	type	SITE
	sequence	W
	description	Interaction with DNA => ECO:0000255\|PROSITE-ProRule:PRU00995
	source	Swiss-Prot : SWS_FT_FI3

6)	chain	A
	residue	481
	type	SITE
	sequence	N
	description	Interaction with DNA => ECO:0000269\|PubMed:20485342
	source	Swiss-Prot : SWS_FT_FI4

7)	chain	A
	residue	701
	type	SITE
	sequence	K
	description	Interaction with DNA => ECO:0000269\|PubMed:20485342
	source	Swiss-Prot : SWS_FT_FI4

8)	chain	A
	residue	782
	type	SITE
	sequence	R
	description	Transition state stabilizer => ECO:0000269\|PubMed:20485342
	source	Swiss-Prot : SWS_FT_FI5

9)	chain	A
	residue	834
	type	SITE
	sequence	I
	description	Important for DNA bending; intercalates between base pairs of target DNA => ECO:0000269\|PubMed:18097402
	source	Swiss-Prot : SWS_FT_FI6

10)	chain	A
	residue	448-456
	type	prosite
	sequence	LTEGDSALS
	description	TOPOISOMERASE_II DNA topoisomerase II signature. LTEGDSALS
	source	prosite : PS00177

11)	chain	A
	residue	527
	type	BINDING
	sequence	D
	description	BINDING => ECO:0000255\|PROSITE-ProRule:PRU00995, ECO:0000269\|PubMed:18097402
	source	Swiss-Prot : SWS_FT_FI2

12)	chain	A
	residue	529
	type	BINDING
	sequence	D
	description	BINDING => ECO:0000255\|PROSITE-ProRule:PRU00995, ECO:0000269\|PubMed:18097402
	source	Swiss-Prot : SWS_FT_FI2

13)	chain	A
	residue	450
	type	BINDING
	sequence	E
	description	BINDING => ECO:0000255\|PROSITE-ProRule:PRU00995, ECO:0000269\|PubMed:18097402
	source	Swiss-Prot : SWS_FT_FI2