eF-site ID 1bdm-AB PDB Code 1bdm Chain A, B click to enlarge Title THE STRUCTURE AT 1.8 ANGSTROMS RESOLUTION OF A SINGLE SITE MUTANT (T189I) OF MALATE DEHYDROGENASE FROM THERMUS FLAVUS WITH INCREASED ENZYMATIC ACTIVITY Classification OXIDOREDUCTASE(NAD(A)-CHOH(D)) Compound MALATE DEHYDROGENASE Source null (MDH_THETH) Sequence A:  MKAPVRVAVTGAAGQIGYSLLFRIAAGEMLGKDQPVILQL LEIPQAMKALEGVVMELEDCAFPLLAGLEATDDPDVAFKD ADYALLVGAAPRLQVNGKIFTEQGRALAEVAKKDVKVLVV GNPANTNALIAYKNAPGLNPRNFTAMTRLDHNRAKAQLAK KTGTGVDRIRRMTVWGNHSSIMFPDLFHAEVDGRPALELV DMEWYEKVFIPTVAQRGAAIIQARGASSAASAANAAIEHI RDWALGTPEGDWVSMAVPSQGEYGIPEGIVYSFPVTAKDG AYRVVEGLEINEFARKRMEITAQELLDEMEQVKALGLI B:  MKAPVRVAVTGAAGQIGYSLLFRIAAGEMLGKDQPVILQL LEIPQAMKALEGVVMELEDCAFPLLAGLEATDDPDVAFKD ADYALLVGAAPRKAGMERRDLLQVNGKIFTEQGRALAEVA KKDVKVLVVGNPANTNALIAYKNAPGLNPRNFTAMTRLDH NRAKAQLAKKTGTGVDRIRRMTVWGNHSSIMFPDLFHAEV DGRPALELVDMEWYEKVFIPTVAQRGAAIIQARGASSAAS AANAAIEHIRDWALGTPEGDWVSMAVPSQGEYGIPEGIVY SFPVTAKDGAYRVVEGLEINEFARKRMEITAQELLDEMEQ VKALGLI Description Functional site 1) chain A residue 10 type sequence G description BINDING SITE FOR RESIDUE NAX A 334 source : AC1 2) chain A residue 13 type sequence G description BINDING SITE FOR RESIDUE NAX A 334 source : AC1 3) chain A residue 14 type sequence Q description BINDING SITE FOR RESIDUE NAX A 334 source : AC1 4) chain A residue 15 type sequence I description BINDING SITE FOR RESIDUE NAX A 334 source : AC1 5) chain A residue 41 type sequence E description BINDING SITE FOR RESIDUE NAX A 334 source : AC1 6) chain A residue 42 type sequence I description BINDING SITE FOR RESIDUE NAX A 334 source : AC1 7) chain A residue 86 type sequence V description BINDING SITE FOR RESIDUE NAX A 334 source : AC1 8) chain A residue 87 type sequence G description BINDING SITE FOR RESIDUE NAX A 334 source : AC1 9) chain A residue 88 type sequence A description BINDING SITE FOR RESIDUE NAX A 334 source : AC1 10) chain A residue 111 type sequence Q description BINDING SITE FOR RESIDUE NAX A 334 source : AC1 11) chain A residue 128 type sequence V description BINDING SITE FOR RESIDUE NAX A 334 source : AC1 12) chain A residue 129 type sequence G description BINDING SITE FOR RESIDUE NAX A 334 source : AC1 13) chain A residue 130 type sequence N description BINDING SITE FOR RESIDUE NAX A 334 source : AC1 14) chain A residue 154 type sequence M description BINDING SITE FOR RESIDUE NAX A 334 source : AC1 15) chain A residue 186 type sequence H description BINDING SITE FOR RESIDUE NAX A 334 source : AC1 16) chain A residue 245 type sequence A description BINDING SITE FOR RESIDUE NAX A 334 source : AC1 17) chain B residue 10 type sequence G description BINDING SITE FOR RESIDUE NAX B 334 source : AC2 18) chain B residue 13 type sequence G description BINDING SITE FOR RESIDUE NAX B 334 source : AC2 19) chain B residue 14 type sequence Q description BINDING SITE FOR RESIDUE NAX B 334 source : AC2 20) chain B residue 15 type sequence I description BINDING SITE FOR RESIDUE NAX B 334 source : AC2 21) chain B residue 41 type sequence E description BINDING SITE FOR RESIDUE NAX B 334 source : AC2 22) chain B residue 42 type sequence I description BINDING SITE FOR RESIDUE NAX B 334 source : AC2 23) chain B residue 86 type sequence V description BINDING SITE FOR RESIDUE NAX B 334 source : AC2 24) chain B residue 87 type sequence G description BINDING SITE FOR RESIDUE NAX B 334 source : AC2 25) chain B residue 88 type sequence A description BINDING SITE FOR RESIDUE NAX B 334 source : AC2 26) chain B residue 107 type sequence I description BINDING SITE FOR RESIDUE NAX B 334 source : AC2 27) chain B residue 111 type sequence Q description BINDING SITE FOR RESIDUE NAX B 334 source : AC2 28) chain B residue 128 type sequence V description BINDING SITE FOR RESIDUE NAX B 334 source : AC2 29) chain B residue 129 type sequence G description BINDING SITE FOR RESIDUE NAX B 334 source : AC2 30) chain B residue 130 type sequence N description BINDING SITE FOR RESIDUE NAX B 334 source : AC2 31) chain B residue 154 type sequence M description BINDING SITE FOR RESIDUE NAX B 334 source : AC2 32) chain B residue 186 type sequence H description BINDING SITE FOR RESIDUE NAX B 334 source : AC2 33) chain B residue 245 type sequence A description BINDING SITE FOR RESIDUE NAX B 334 source : AC2 34) chain A residue 186 type ACT_SITE sequence H description Proton acceptor => ECO:0000255|HAMAP-Rule:MF_01517 source Swiss-Prot : SWS_FT_FI1 35) chain B residue 186 type ACT_SITE sequence H description Proton acceptor => ECO:0000255|HAMAP-Rule:MF_01517 source Swiss-Prot : SWS_FT_FI1 36) chain A residue 128 type BINDING sequence V description BINDING => ECO:0000255|HAMAP-Rule:MF_01517, ECO:0000269|PubMed:16009341, ECO:0000269|PubMed:8471603 source Swiss-Prot : SWS_FT_FI2 37) chain B residue 10 type BINDING sequence G description BINDING => ECO:0000255|HAMAP-Rule:MF_01517, ECO:0000269|PubMed:16009341, ECO:0000269|PubMed:8471603 source Swiss-Prot : SWS_FT_FI2 38) chain B residue 128 type BINDING sequence V description BINDING => ECO:0000255|HAMAP-Rule:MF_01517, ECO:0000269|PubMed:16009341, ECO:0000269|PubMed:8471603 source Swiss-Prot : SWS_FT_FI2 39) chain A residue 10 type BINDING sequence G description BINDING => ECO:0000255|HAMAP-Rule:MF_01517, ECO:0000269|PubMed:16009341, ECO:0000269|PubMed:8471603 source Swiss-Prot : SWS_FT_FI2 40) chain A residue 161 type BINDING sequence R description BINDING => ECO:0000255|HAMAP-Rule:MF_01517 source Swiss-Prot : SWS_FT_FI3 41) chain B residue 91 type BINDING sequence R description BINDING => ECO:0000255|HAMAP-Rule:MF_01517 source Swiss-Prot : SWS_FT_FI3 42) chain B residue 97 type BINDING sequence R description BINDING => ECO:0000255|HAMAP-Rule:MF_01517 source Swiss-Prot : SWS_FT_FI3 43) chain B residue 104 type BINDING sequence N description BINDING => ECO:0000255|HAMAP-Rule:MF_01517 source Swiss-Prot : SWS_FT_FI3 44) chain B residue 161 type BINDING sequence R description BINDING => ECO:0000255|HAMAP-Rule:MF_01517 source Swiss-Prot : SWS_FT_FI3 45) chain B residue 130 type BINDING sequence N description BINDING => ECO:0000255|HAMAP-Rule:MF_01517 source Swiss-Prot : SWS_FT_FI3 46) chain A residue 91 type BINDING sequence R description BINDING => ECO:0000255|HAMAP-Rule:MF_01517 source Swiss-Prot : SWS_FT_FI3 47) chain A residue 104 type BINDING sequence N description BINDING => ECO:0000255|HAMAP-Rule:MF_01517 source Swiss-Prot : SWS_FT_FI3 48) chain A residue 130 type BINDING sequence N description BINDING => ECO:0000255|HAMAP-Rule:MF_01517 source Swiss-Prot : SWS_FT_FI3 49) chain B residue 111 type BINDING sequence Q description BINDING => ECO:0000255|HAMAP-Rule:MF_01517, ECO:0000269|PubMed:16009341 source Swiss-Prot : SWS_FT_FI4 50) chain A residue 111 type BINDING sequence Q description BINDING => ECO:0000255|HAMAP-Rule:MF_01517, ECO:0000269|PubMed:16009341 source Swiss-Prot : SWS_FT_FI4 51) chain A residue 154-166 type prosite sequence MTRLDHNRAKAQL description MDH Malate dehydrogenase active site signature. MTRLDhnRAkaqL source prosite : PS00068

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