eF-site ID 1ajd-A PDB Code 1ajd Chain A click to enlarge Title THREE-DIMENSIONAL STRUCTURE OF THE D153G MUTANT OF E. COLI ALKALINE PHOSPHATASE: A MUTANT WITH WEAKER MAGNESIUM BINDING AND INCREASED CATALYTIC ACTIVITY Classification NON SPECIFIC MONO-ESTERASE Compound ALKALINE PHOSPHATASE INTERMEDIATE II OF HOLO ENZYME Source Escherichia coli (strain K12) (PPB_ECOLI) Sequence A:  TPEMPVLENRAAQGDITAPGGARRLTGDQTAALRDSLSDK PAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGAL GVDIHEKDHPTILEMAKAAGLATGNVSTAELQGATPAALV AHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARA DVTLGGGAKTFAETATAGEWQGKTLREQAEARGYQLVSDA ASLNSVTEANQQKPLLGLFADGNMPVRWLGPKATYHGNID KPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQ VEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEG NTLVIVTADHAHASQIVAPDTKAPGLTQALNTKDGAVMVM SYGNSEEDSQEHTGSQLRIAAYGPHAANVVGLTDQTDLFY TMKAALGLK Description Functional site 1) chain A residue 327 type sequence D description BINDING SITE FOR RESIDUE ZN A 450 source : AC1 2) chain A residue 331 type sequence H description BINDING SITE FOR RESIDUE ZN A 450 source : AC1 3) chain A residue 412 type sequence H description BINDING SITE FOR RESIDUE ZN A 450 source : AC1 4) chain A residue 51 type sequence D description BINDING SITE FOR RESIDUE ZN A 451 source : AC2 5) chain A residue 102 type sequence S description BINDING SITE FOR RESIDUE ZN A 451 source : AC2 6) chain A residue 327 type sequence D description BINDING SITE FOR RESIDUE ZN A 451 source : AC2 7) chain A residue 369 type sequence D description BINDING SITE FOR RESIDUE ZN A 451 source : AC2 8) chain A residue 370 type sequence H description BINDING SITE FOR RESIDUE ZN A 451 source : AC2 9) chain A residue 412 type sequence H description BINDING SITE FOR RESIDUE ZN A 451 source : AC2 10) chain A residue 51 type sequence D description BINDING SITE FOR RESIDUE MG A 452 source : AC3 11) chain A residue 155 type sequence T description BINDING SITE FOR RESIDUE MG A 452 source : AC3 12) chain A residue 322 type sequence E description BINDING SITE FOR RESIDUE MG A 452 source : AC3 13) chain A residue 51 type BINDING sequence D description source Swiss-Prot : SWS_FT_FI2 14) chain A residue 153 type BINDING sequence G description source Swiss-Prot : SWS_FT_FI2 15) chain A residue 155 type BINDING sequence T description source Swiss-Prot : SWS_FT_FI2 16) chain A residue 322 type BINDING sequence E description source Swiss-Prot : SWS_FT_FI2 17) chain A residue 327 type BINDING sequence D description source Swiss-Prot : SWS_FT_FI2 18) chain A residue 331 type BINDING sequence H description source Swiss-Prot : SWS_FT_FI2 19) chain A residue 369 type BINDING sequence D description source Swiss-Prot : SWS_FT_FI2 20) chain A residue 370 type BINDING sequence H description source Swiss-Prot : SWS_FT_FI2 21) chain A residue 412 type BINDING sequence H description source Swiss-Prot : SWS_FT_FI2 22) chain A residue 102 type ACT_SITE sequence S description Phosphoserine intermediate source Swiss-Prot : SWS_FT_FI1 23) chain A residue 51 type catalytic sequence D description 44 source MCSA : MCSA1 24) chain A residue 369 type catalytic sequence D description 44 source MCSA : MCSA1 25) chain A residue 370 type catalytic sequence H description 44 source MCSA : MCSA1 26) chain A residue 412 type catalytic sequence H description 44 source MCSA : MCSA1 27) chain A residue 102 type catalytic sequence S description 44 source MCSA : MCSA1 28) chain A residue 153 type catalytic sequence G description 44 source MCSA : MCSA1 29) chain A residue 155 type catalytic sequence T description 44 source MCSA : MCSA1 30) chain A residue 166 type catalytic sequence R description 44 source MCSA : MCSA1 31) chain A residue 322 type catalytic sequence E description 44 source MCSA : MCSA1 32) chain A residue 327 type catalytic sequence D description 44 source MCSA : MCSA1 33) chain A residue 328 type catalytic sequence K description 44 source MCSA : MCSA1 34) chain A residue 331 type catalytic sequence H description 44 source MCSA : MCSA1 35) chain A residue 99-107 type prosite sequence VTDSAASAT description ALKALINE_PHOSPHATASE Alkaline phosphatase active site. VtDSAASAT source prosite : PS00123

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