|
|
eF-site ID
|
1a3a-ABCD |
|
PDB Code
|
1a3a |
|
Chain
|
A, B, C, D |
|
click to enlarge
|
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Title
|
CRYSTAL STRUCTURE OF IIA MANNITOL FROM ESCHERICHIA COLI |
|
Classification
|
PHOSPHOTRANSFERASE |
|
Compound
|
MANNITOL-SPECIFIC EII |
|
Source
|
Escherichia coli (strain K12) (PTM3C_ECOLI) |
|
|
Sequence
|
A: |
LFKLGAENIFLGRKAATKEEAIRFAGEQLVKGGYVEPEYV
QAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKTGVVFCQ
YPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALD
DESVIERLAHTTSVDEVLELLAGRK
|
| B: |
FKLGAENIFLGRKAATKEEAIRFAGEQLVKGGYVEPEYVQ
AMLDREKLTPTYLGESIAVPHGTVEAKDRVLKTGVVFCQY
PEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDD
ESVIERLAHTTSVDEVLELLA
|
| C: |
NLFKLGAENIFLGRKAATKEEAIRFAGEQLVKGGYVEPEY
VQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKTGVVFC
QYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNAL
DDESVIERLAHTTSVDEVLELLAGRK
|
| D: |
LFKLGAENIFLGRKAATKEEAIRFAGEQLVKGGYVEPEYV
QAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKTGVVFCQ
YPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALD
DESVIERLAHTTSVDEVLELLAGR
|
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Description
|
|
Functional site
|
|
|
1)
|
chain |
A |
| residue |
65 |
| type |
ACT_SITE |
| sequence |
H
|
| description |
Tele-phosphohistidine intermediate; for EIIA activity => ECO:0000255|PROSITE-ProRule:PRU00417, ECO:0000305|PubMed:12202490, ECO:0000305|PubMed:16443929, ECO:0000305|PubMed:9551558
|
| source |
Swiss-Prot : SWS_FT_FI1
|
|
|
2)
|
chain |
B |
| residue |
65 |
| type |
ACT_SITE |
| sequence |
H
|
| description |
Tele-phosphohistidine intermediate; for EIIA activity => ECO:0000255|PROSITE-ProRule:PRU00417, ECO:0000305|PubMed:12202490, ECO:0000305|PubMed:16443929, ECO:0000305|PubMed:9551558
|
| source |
Swiss-Prot : SWS_FT_FI1
|
|
|
3)
|
chain |
C |
| residue |
65 |
| type |
ACT_SITE |
| sequence |
H
|
| description |
Tele-phosphohistidine intermediate; for EIIA activity => ECO:0000255|PROSITE-ProRule:PRU00417, ECO:0000305|PubMed:12202490, ECO:0000305|PubMed:16443929, ECO:0000305|PubMed:9551558
|
| source |
Swiss-Prot : SWS_FT_FI1
|
|
|
4)
|
chain |
D |
| residue |
65 |
| type |
ACT_SITE |
| sequence |
H
|
| description |
Tele-phosphohistidine intermediate; for EIIA activity => ECO:0000255|PROSITE-ProRule:PRU00417, ECO:0000305|PubMed:12202490, ECO:0000305|PubMed:16443929, ECO:0000305|PubMed:9551558
|
| source |
Swiss-Prot : SWS_FT_FI1
|
|
|
5)
|
chain |
A |
| residue |
49 |
| type |
SITE |
| sequence |
R
|
| description |
Stabilizes the transition state in the phosphoryl transfer from HPr to EIIA => ECO:0000269|PubMed:9551558
|
| source |
Swiss-Prot : SWS_FT_FI2
|
|
|
6)
|
chain |
B |
| residue |
49 |
| type |
SITE |
| sequence |
R
|
| description |
Stabilizes the transition state in the phosphoryl transfer from HPr to EIIA => ECO:0000269|PubMed:9551558
|
| source |
Swiss-Prot : SWS_FT_FI2
|
|
|
7)
|
chain |
C |
| residue |
49 |
| type |
SITE |
| sequence |
R
|
| description |
Stabilizes the transition state in the phosphoryl transfer from HPr to EIIA => ECO:0000269|PubMed:9551558
|
| source |
Swiss-Prot : SWS_FT_FI2
|
|
|
8)
|
chain |
D |
| residue |
49 |
| type |
SITE |
| sequence |
R
|
| description |
Stabilizes the transition state in the phosphoryl transfer from HPr to EIIA => ECO:0000269|PubMed:9551558
|
| source |
Swiss-Prot : SWS_FT_FI2
|
|
|
9)
|
chain |
A |
| residue |
65 |
| type |
MOD_RES |
| sequence |
H
|
| description |
Phosphohistidine; by HPr => ECO:0000269|PubMed:3142516, ECO:0000305|PubMed:12202490, ECO:0000305|PubMed:16443929, ECO:0000305|PubMed:2407724, ECO:0000305|PubMed:9551558
|
| source |
Swiss-Prot : SWS_FT_FI3
|
|
|
10)
|
chain |
B |
| residue |
65 |
| type |
MOD_RES |
| sequence |
H
|
| description |
Phosphohistidine; by HPr => ECO:0000269|PubMed:3142516, ECO:0000305|PubMed:12202490, ECO:0000305|PubMed:16443929, ECO:0000305|PubMed:2407724, ECO:0000305|PubMed:9551558
|
| source |
Swiss-Prot : SWS_FT_FI3
|
|
|
11)
|
chain |
C |
| residue |
65 |
| type |
MOD_RES |
| sequence |
H
|
| description |
Phosphohistidine; by HPr => ECO:0000269|PubMed:3142516, ECO:0000305|PubMed:12202490, ECO:0000305|PubMed:16443929, ECO:0000305|PubMed:2407724, ECO:0000305|PubMed:9551558
|
| source |
Swiss-Prot : SWS_FT_FI3
|
|
|
12)
|
chain |
D |
| residue |
65 |
| type |
MOD_RES |
| sequence |
H
|
| description |
Phosphohistidine; by HPr => ECO:0000269|PubMed:3142516, ECO:0000305|PubMed:12202490, ECO:0000305|PubMed:16443929, ECO:0000305|PubMed:2407724, ECO:0000305|PubMed:9551558
|
| source |
Swiss-Prot : SWS_FT_FI3
|
|
|
13)
|
chain |
A |
| residue |
50-66 |
| type |
prosite |
| sequence |
EKLTPTYLGESIAVPHG
|
| description |
PTS_EIIA_TYPE_2_HIS PTS EIIA domains phosphorylation site signature 2. EkltptyLGesIAVPHG
|
| source |
prosite : PS00372
|
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