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eF-site ID
|
1a31-A |
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PDB Code
|
1a31 |
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Chain
|
A |
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click to enlarge
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Title
|
HUMAN RECONSTITUTED DNA TOPOISOMERASE I IN COVALENT COMPLEX WITH A 22 BASE PAIR DNA DUPLEX |
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Classification
|
ISOMERASE/DNA |
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Compound
|
DNA (5'-D(*AP*AP*AP*AP*AP*GP*AP*CP*5IUP*5IU*TP*GP*AP*AP*AP*AP*AP*5IUP*5IUP*5IUP*5IUP*T)-3') |
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Source
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Homo sapiens (Human) (1A31) |
|
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Sequence
|
A: |
IKWKFLEHKGPVFAPPYEPLPENVKFYYDGKVMKLSPKAE
EVATFFAKMLDHEYTTKEIFRKNFFKDWRKEMTNEEKNII
TNLSKCDFTQMSQYFKAQTEARKQMSKEEKLKIKEENEKL
LKEYGFCIMDNHKERIANFKIEPPGLFRGRGNHPKMGMLK
RRIMPEDIIINCSKDAKVPSPPPGHKWKEVRHDNKVTWLV
SWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCV
DKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKE
EGETADTVGCCSLRVEHINLHPELDGQEYVVEFDFLGKDS
IRYYNKVPVEKRVFKNLQLFMENKQPEDDLFDRLNTGILN
KHLQDLMEGLTAKVFRTYNASITLQQQLKELTAPDENIPA
KILSYNRANRAVKLNXLDPRITVAWCKKWGVPIEKIYNKT
QREKFAWAIDMADEDYEF
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Description
|
(1) |
TOPOISOMERASE I (E.C.5.99.1.2)/DNA COMPLEX
|
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Functional site
|
|
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1)
|
chain |
A |
| residue |
316 |
| type |
SITE |
| sequence |
R
|
| description |
Interaction with DNA
|
| source |
Swiss-Prot : SWS_FT_FI2
|
|
|
2)
|
chain |
A |
| residue |
364 |
| type |
SITE |
| sequence |
R
|
| description |
Interaction with DNA
|
| source |
Swiss-Prot : SWS_FT_FI2
|
|
|
3)
|
chain |
A |
| residue |
412 |
| type |
SITE |
| sequence |
W
|
| description |
Interaction with DNA
|
| source |
Swiss-Prot : SWS_FT_FI2
|
|
|
4)
|
chain |
A |
| residue |
443 |
| type |
SITE |
| sequence |
K
|
| description |
Interaction with DNA
|
| source |
Swiss-Prot : SWS_FT_FI2
|
|
|
5)
|
chain |
A |
| residue |
501 |
| type |
SITE |
| sequence |
T
|
| description |
Interaction with DNA
|
| source |
Swiss-Prot : SWS_FT_FI2
|
|
|
6)
|
chain |
A |
| residue |
532 |
| type |
SITE |
| sequence |
K
|
| description |
Interaction with DNA
|
| source |
Swiss-Prot : SWS_FT_FI2
|
|
|
7)
|
chain |
A |
| residue |
574 |
| type |
SITE |
| sequence |
N
|
| description |
Interaction with DNA
|
| source |
Swiss-Prot : SWS_FT_FI2
|
|
|
8)
|
chain |
A |
| residue |
549 |
| type |
CROSSLNK |
| sequence |
K
|
| description |
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733
|
| source |
Swiss-Prot : SWS_FT_FI5
|
|
|
9)
|
chain |
A |
| residue |
336 |
| type |
CROSSLNK |
| sequence |
K
|
| description |
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:25755297, ECO:0007744|PubMed:28112733
|
| source |
Swiss-Prot : SWS_FT_FI6
|
|
|
10)
|
chain |
A |
| residue |
280 |
| type |
MOD_RES |
| sequence |
K
|
| description |
N6-acetyllysine => ECO:0007744|PubMed:19608861
|
| source |
Swiss-Prot : SWS_FT_FI3
|
|
|
11)
|
chain |
A |
| residue |
506 |
| type |
MOD_RES |
| sequence |
S
|
| description |
Phosphoserine; by CK2 => ECO:0000269|PubMed:23185622
|
| source |
Swiss-Prot : SWS_FT_FI4
|
|
|
12)
|
chain |
A |
| residue |
723 |
| type |
ACT_SITE |
| sequence |
X
|
| description |
O-(3'-phospho-DNA)-tyrosine intermediate => ECO:0000255|PROSITE-ProRule:PRU01382, ECO:0000255|PROSITE-ProRule:PRU10130, ECO:0000269|PubMed:10841763, ECO:0000269|PubMed:12426403, ECO:0000269|PubMed:12533542, ECO:0000269|PubMed:15165849, ECO:0000269|PubMed:15801827, ECO:0000269|PubMed:16033260, ECO:0000269|PubMed:9488644
|
| source |
Swiss-Prot : SWS_FT_FI1
|
|
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