eF-site ID 12as-AB PDB Code 12as Chain A, B click to enlarge Title ASPARAGINE SYNTHETASE MUTANT C51A, C315A COMPLEXED WITH L-ASPARAGINE AND AMP Classification LIGASE Compound ASPARAGINE SYNTHETASE Source null (ASNA_ECOLI) Sequence A:  AYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDG TQDNLSGAEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQ HDFSAGEGLYTHMKALRPDEDRLSPLHSVYVDQWDWERVM GDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPFLPD QIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGG KLSDGHRHDVRAPDYDDWSTPSELGHAGLNGDILVWNPVL EDAFELSSMGIRVDADTLKHQLALTGDEDRLELEWHQALL RGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQAGVWPAAVR ESVPSLL B:  AYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDG TQDNLSGAEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQ HDFSAGEGLYTHMKALRPDEDRLSPLHSVYVDQWDWERVM GDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPFLPD QIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGG KLSDGHRHDVRAPDYDDWSTPSELGHAGLNGDILVWNPVL EDAFELSSMGIRVDADTLKHQLALTGDEDRLELEWHQALL RGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQAGVWPAAVR ESVPSLL Description Functional site 1) chain A residue 46 type sequence D description BINDING SITE FOR RESIDUE ASN A 331 source : AC1 2) chain A residue 72 type sequence S description BINDING SITE FOR RESIDUE ASN A 331 source : AC1 3) chain A residue 74 type sequence A description BINDING SITE FOR RESIDUE ASN A 331 source : AC1 4) chain A residue 77 type sequence K description BINDING SITE FOR RESIDUE ASN A 331 source : AC1 5) chain A residue 116 type sequence Q description BINDING SITE FOR RESIDUE ASN A 331 source : AC1 6) chain A residue 118 type sequence D description BINDING SITE FOR RESIDUE ASN A 331 source : AC1 7) chain A residue 218 type sequence Y description BINDING SITE FOR RESIDUE ASN A 331 source : AC1 8) chain A residue 251 type sequence S description BINDING SITE FOR RESIDUE ASN A 331 source : AC1 9) chain A residue 255 type sequence R description BINDING SITE FOR RESIDUE ASN A 331 source : AC1 10) chain A residue 293 type sequence G description BINDING SITE FOR RESIDUE ASN A 331 source : AC1 11) chain A residue 294 type sequence G description BINDING SITE FOR RESIDUE ASN A 331 source : AC1 12) chain B residue 46 type sequence D description BINDING SITE FOR RESIDUE ASN B 331 source : AC2 13) chain B residue 72 type sequence S description BINDING SITE FOR RESIDUE ASN B 331 source : AC2 14) chain B residue 74 type sequence A description BINDING SITE FOR RESIDUE ASN B 331 source : AC2 15) chain B residue 77 type sequence K description BINDING SITE FOR RESIDUE ASN B 331 source : AC2 16) chain B residue 116 type sequence Q description BINDING SITE FOR RESIDUE ASN B 331 source : AC2 17) chain B residue 118 type sequence D description BINDING SITE FOR RESIDUE ASN B 331 source : AC2 18) chain B residue 218 type sequence Y description BINDING SITE FOR RESIDUE ASN B 331 source : AC2 19) chain B residue 219 type sequence D description BINDING SITE FOR RESIDUE ASN B 331 source : AC2 20) chain B residue 251 type sequence S description BINDING SITE FOR RESIDUE ASN B 331 source : AC2 21) chain B residue 255 type sequence R description BINDING SITE FOR RESIDUE ASN B 331 source : AC2 22) chain B residue 292 type sequence G description BINDING SITE FOR RESIDUE ASN B 331 source : AC2 23) chain B residue 293 type sequence G description BINDING SITE FOR RESIDUE ASN B 331 source : AC2 24) chain A residue 100 type sequence R description BINDING SITE FOR RESIDUE AMP A 332 source : AC3 25) chain A residue 103 type sequence E description BINDING SITE FOR RESIDUE AMP A 332 source : AC3 26) chain A residue 109 type sequence L description BINDING SITE FOR RESIDUE AMP A 332 source : AC3 27) chain A residue 110 type sequence H description BINDING SITE FOR RESIDUE AMP A 332 source : AC3 28) chain A residue 111 type sequence S description BINDING SITE FOR RESIDUE AMP A 332 source : AC3 29) chain A residue 114 type sequence V description BINDING SITE FOR RESIDUE AMP A 332 source : AC3 30) chain A residue 116 type sequence Q description BINDING SITE FOR RESIDUE AMP A 332 source : AC3 31) chain A residue 248 type sequence E description BINDING SITE FOR RESIDUE AMP A 332 source : AC3 32) chain A residue 249 type sequence L description BINDING SITE FOR RESIDUE AMP A 332 source : AC3 33) chain A residue 250 type sequence S description BINDING SITE FOR RESIDUE AMP A 332 source : AC3 34) chain A residue 251 type sequence S description BINDING SITE FOR RESIDUE AMP A 332 source : AC3 35) chain A residue 294 type sequence G description BINDING SITE FOR RESIDUE AMP A 332 source : AC3 36) chain A residue 296 type sequence G description BINDING SITE FOR RESIDUE AMP A 332 source : AC3 37) chain A residue 299 type sequence R description BINDING SITE FOR RESIDUE AMP A 332 source : AC3 38) chain A residue 310 type sequence I description BINDING SITE FOR RESIDUE AMP A 332 source : AC3 39) chain B residue 100 type sequence R description BINDING SITE FOR RESIDUE AMP B 332 source : AC4 40) chain B residue 103 type sequence E description BINDING SITE FOR RESIDUE AMP B 332 source : AC4 41) chain B residue 109 type sequence L description BINDING SITE FOR RESIDUE AMP B 332 source : AC4 42) chain B residue 110 type sequence H description BINDING SITE FOR RESIDUE AMP B 332 source : AC4 43) chain B residue 111 type sequence S description BINDING SITE FOR RESIDUE AMP B 332 source : AC4 44) chain B residue 114 type sequence V description BINDING SITE FOR RESIDUE AMP B 332 source : AC4 45) chain B residue 116 type sequence Q description BINDING SITE FOR RESIDUE AMP B 332 source : AC4 46) chain B residue 248 type sequence E description BINDING SITE FOR RESIDUE AMP B 332 source : AC4 47) chain B residue 249 type sequence L description BINDING SITE FOR RESIDUE AMP B 332 source : AC4 48) chain B residue 250 type sequence S description BINDING SITE FOR RESIDUE AMP B 332 source : AC4 49) chain B residue 251 type sequence S description BINDING SITE FOR RESIDUE AMP B 332 source : AC4 50) chain B residue 296 type sequence G description BINDING SITE FOR RESIDUE AMP B 332 source : AC4 51) chain B residue 299 type sequence R description BINDING SITE FOR RESIDUE AMP B 332 source : AC4 52) chain A residue 46 type catalytic sequence D description 75 source MCSA : MCSA1 53) chain A residue 100 type catalytic sequence R description 75 source MCSA : MCSA1 54) chain A residue 116 type catalytic sequence Q description 75 source MCSA : MCSA1 55) chain A residue 235 type catalytic sequence D description 75 source MCSA : MCSA1 56) chain A residue 248 type catalytic sequence E description 75 source MCSA : MCSA1 57) chain A residue 251 type catalytic sequence S description 75 source MCSA : MCSA1 58) chain B residue 46 type catalytic sequence D description 75 source MCSA : MCSA2 59) chain B residue 100 type catalytic sequence R description 75 source MCSA : MCSA2 60) chain B residue 116 type catalytic sequence Q description 75 source MCSA : MCSA2 61) chain B residue 235 type catalytic sequence D description 75 source MCSA : MCSA2 62) chain B residue 248 type catalytic sequence E description 75 source MCSA : MCSA2 63) chain B residue 251 type catalytic sequence S description 75 source MCSA : MCSA2

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