eF-site ID 11as-AB PDB Code 11as Chain A, B click to enlarge Title ASPARAGINE SYNTHETASE MUTANT C51A, C315A COMPLEXED WITH L-ASPARAGINE Classification LIGASE Compound ASPARAGINE SYNTHETASE Source Escherichia coli (strain K12) (ASNA_ECOLI) Sequence A:  AYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDG TQDNLSGAEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQ HDFSAGEGLYTHMKALRPDEDRLSPLHSVYVDQWDWERVM GDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPFLPD QIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGG KLSDGHRHDVRAPDYDDWSTPSELGHAGLNGDILVWNPVL EDAFELSSMGIRVDADTLKHQLALTGDEDRLELEWHQALL RGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQAGVWPAAVR ESVPSLL B:  AYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDG TQDNLSGAEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQ HDFSAGEGLYTHMKALRPDEDRLSPLHSVYVDQWDWERVM GDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPFLPD QIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGG KLSDGHRHDVRAPDYDDWSTPSELGHAGLNGDILVWNPVL EDAFELSSMGIRVDADTLKHQLALTGDEDRLELEWHQALL RGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQAGVWPAAVR ESVPSLL Description Functional site 1) chain A residue 46 type sequence D description BINDING SITE FOR RESIDUE ASN A 331 source : AC1 2) chain A residue 72 type sequence S description BINDING SITE FOR RESIDUE ASN A 331 source : AC1 3) chain A residue 74 type sequence A description BINDING SITE FOR RESIDUE ASN A 331 source : AC1 4) chain A residue 77 type sequence K description BINDING SITE FOR RESIDUE ASN A 331 source : AC1 5) chain A residue 100 type sequence R description BINDING SITE FOR RESIDUE ASN A 331 source : AC1 6) chain A residue 116 type sequence Q description BINDING SITE FOR RESIDUE ASN A 331 source : AC1 7) chain A residue 118 type sequence D description BINDING SITE FOR RESIDUE ASN A 331 source : AC1 8) chain A residue 218 type sequence Y description BINDING SITE FOR RESIDUE ASN A 331 source : AC1 9) chain A residue 251 type sequence S description BINDING SITE FOR RESIDUE ASN A 331 source : AC1 10) chain A residue 255 type sequence R description BINDING SITE FOR RESIDUE ASN A 331 source : AC1 11) chain B residue 46 type sequence D description BINDING SITE FOR RESIDUE ASN B 331 source : AC2 12) chain B residue 72 type sequence S description BINDING SITE FOR RESIDUE ASN B 331 source : AC2 13) chain B residue 77 type sequence K description BINDING SITE FOR RESIDUE ASN B 331 source : AC2 14) chain B residue 118 type sequence D description BINDING SITE FOR RESIDUE ASN B 331 source : AC2 15) chain B residue 218 type sequence Y description BINDING SITE FOR RESIDUE ASN B 331 source : AC2 16) chain B residue 251 type sequence S description BINDING SITE FOR RESIDUE ASN B 331 source : AC2 17) chain B residue 255 type sequence R description BINDING SITE FOR RESIDUE ASN B 331 source : AC2 18) chain B residue 293 type sequence G description BINDING SITE FOR RESIDUE ASN B 331 source : AC2 19) chain A residue 46 type catalytic sequence D description 75 source MCSA : MCSA1 20) chain A residue 100 type catalytic sequence R description 75 source MCSA : MCSA1 21) chain A residue 116 type catalytic sequence Q description 75 source MCSA : MCSA1 22) chain A residue 235 type catalytic sequence D description 75 source MCSA : MCSA1 23) chain A residue 248 type catalytic sequence E description 75 source MCSA : MCSA1 24) chain A residue 251 type catalytic sequence S description 75 source MCSA : MCSA1 25) chain B residue 46 type catalytic sequence D description 75 source MCSA : MCSA2 26) chain B residue 100 type catalytic sequence R description 75 source MCSA : MCSA2 27) chain B residue 116 type catalytic sequence Q description 75 source MCSA : MCSA2 28) chain B residue 235 type catalytic sequence D description 75 source MCSA : MCSA2 29) chain B residue 248 type catalytic sequence E description 75 source MCSA : MCSA2 30) chain B residue 251 type catalytic sequence S description 75 source MCSA : MCSA2

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