eF-site ID 117e-B PDB Code 117e Chain B click to enlarge Title THE R78K AND D117E ACTIVE SITE VARIANTS OF SACCHAROMYCES CEREVISIAE SOLUBLE INORGANIC PYROPHOSPHATASE: STRUCTURAL STUDIES AND MECHANISTIC IMPLICATIONS Classification HYDROLASE Compound PROTEIN (INORGANIC PYROPHOSPHATASE) Source Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (IPYR_YEAST) Sequence B:  TYTTRQIGAKNTLEYKVYIEKDGKPVSAFHDIPLYADKEN NIFNMVVEIPRWTNAKLEITKEETLNPIIQDTKKGKLRFV RNCFPHHGYIHNYGAFPQTWEDPNVSHPETKAVGDNEPID VLEIGETIAYTGQVKQVKALGIMALLDEGETDWKVIAIDI NDPLAPKLNDIEDVEKYFPGLLRATNEWFRIYKIPDGKPE NQFAFSGEAKNKKYALDIIKETHDSWKQLIAGKSSDSKGI DLTNVTLPDTPTYSKAASDAIPPASLKADAPIDKSIDKWF FI Description Functional site 1) chain B residue 1117 type sequence E description source : MN5 2) chain B residue 1152 type sequence D description source : MN5 3) chain B residue 1115 type sequence D description source : MN5 4) chain B residue 1120 type sequence D description source : MN5 5) chain B residue 1117 type sequence E description source : MN6 6) chain B residue 1093 type sequence Y description source : MN6 7) chain B residue 1120 type sequence D description source : MN6 8) chain B residue 1152 type sequence D description source : MN8 9) chain B residue 1147 type sequence D description source : MN8 10) chain B residue 1115 type sequence D description BINDING SITE FOR RESIDUE MN B 2005 source : AC5 11) chain B residue 1117 type sequence E description BINDING SITE FOR RESIDUE MN B 2005 source : AC5 12) chain B residue 1120 type sequence D description BINDING SITE FOR RESIDUE MN B 2005 source : AC5 13) chain B residue 1152 type sequence D description BINDING SITE FOR RESIDUE MN B 2005 source : AC5 14) chain B residue 1117 type sequence E description BINDING SITE FOR RESIDUE MN B 2006 source : AC6 15) chain B residue 1120 type sequence D description BINDING SITE FOR RESIDUE MN B 2006 source : AC6 16) chain B residue 1058 type sequence E description BINDING SITE FOR RESIDUE MN B 2007 source : AC7 17) chain B residue 1117 type sequence E description BINDING SITE FOR RESIDUE MN B 2007 source : AC7 18) chain B residue 1147 type sequence D description BINDING SITE FOR RESIDUE MN B 2008 source : AC8 19) chain B residue 1152 type sequence D description BINDING SITE FOR RESIDUE MN B 2008 source : AC8 20) chain B residue 1056 type sequence K description BINDING SITE FOR RESIDUE PO4 B 3004 source : BC2 21) chain B residue 1093 type sequence Y description BINDING SITE FOR RESIDUE PO4 B 3004 source : BC2 22) chain B residue 1115 type sequence D description BINDING SITE FOR RESIDUE PO4 B 3004 source : BC2 23) chain B residue 1117 type sequence E description BINDING SITE FOR RESIDUE PO4 B 3004 source : BC2 24) chain B residue 1120 type sequence D description BINDING SITE FOR RESIDUE PO4 B 3004 source : BC2 25) chain B residue 1152 type sequence D description BINDING SITE FOR RESIDUE PO4 B 3004 source : BC2 26) chain B residue 1154 type sequence K description BINDING SITE FOR RESIDUE PO4 B 3004 source : BC2 27) chain B residue 1090 type ACT_SITE sequence I description Proton donor => ECO:0000269|PubMed:1322842 source Swiss-Prot : SWS_FT_FI1 28) chain B residue 1079 type BINDING sequence F description source Swiss-Prot : SWS_FT_FI2 29) chain B residue 1116 type BINDING sequence N description source Swiss-Prot : SWS_FT_FI2 30) chain B residue 1121 type BINDING sequence V description source Swiss-Prot : SWS_FT_FI2 31) chain B residue 1153 type BINDING sequence W description source Swiss-Prot : SWS_FT_FI2 32) chain B residue 1065 type MOD_RES sequence L description Phosphothreonine => ECO:0007744|PubMed:18407956 source Swiss-Prot : SWS_FT_FI3 33) chain B residue 1251 type MOD_RES sequence P description Phosphothreonine => ECO:0007744|PubMed:15665377, ECO:0007744|PubMed:17330950 source Swiss-Prot : SWS_FT_FI4 34) chain B residue 1266 type MOD_RES sequence L description Phosphoserine => ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198 source Swiss-Prot : SWS_FT_FI5 35) chain B residue 1239 type CROSSLNK sequence G description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0007744|PubMed:22106047 source Swiss-Prot : SWS_FT_FI7 36) chain B residue 1279 type CROSSLNK sequence W description Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0007744|PubMed:22106047 source Swiss-Prot : SWS_FT_FI7

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