8QO9
| Cryo-EM structure of a human spliceosomal B complex protomer | Descriptor: | 116 kDa U5 small nuclear ribonucleoprotein component, MINX pre-mRNA, Microfibrillar-associated protein 1, ... | Authors: | Zhang, Z, Kumar, V, Dybkov, O, Will, C.L, Urlaub, H, Stark, H, Luehrmann, R. | Deposit date: | 2023-09-28 | Release date: | 2024-01-24 | Method: | ELECTRON MICROSCOPY (5.29 Å) | Cite: | New insights into the functions of B complex proteins revealed by cryo-EM of dimerized
spliceosomes To Be Published
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8Q7N
| cryo-EM structure of the human spliceosomal B complex protomer (tri-snRNP core region) | Descriptor: | 116 kDa U5 small nuclear ribonucleoprotein component, MINX pre-mRNA, Microfibrillar-associated protein 1, ... | Authors: | Zhang, Z, Kumar, V, Dybkov, O, Will, C.L, Urlaub, H, Stark, H, Luehrmann, R. | Deposit date: | 2023-08-16 | Release date: | 2024-01-24 | Method: | ELECTRON MICROSCOPY (3.1 Å) | Cite: | New insights into the functions of B complex proteins revealed by cryo-EM of dimerized spliceosomes To Be Published
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6Y53
| human 17S U2 snRNP low resolution part | Descriptor: | HIV Tat-specific factor 1, Probable ATP-dependent RNA helicase DDX46, Small nuclear ribonucleoprotein E, ... | Authors: | Zhang, Z, Will, C.L, Bertram, K, Luehrmann, R, Stark, H. | Deposit date: | 2020-02-24 | Release date: | 2020-06-17 | Last modified: | 2020-07-22 | Method: | ELECTRON MICROSCOPY (7.1 Å) | Cite: | Molecular architecture of the human 17S U2 snRNP. Nature, 583, 2020
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6Y5Q
| human 17S U2 snRNP | Descriptor: | HIV Tat-specific factor 1, PHD finger-like domain-containing protein 5A, Probable ATP-dependent RNA helicase DDX46, ... | Authors: | Zhang, Z, Will, C.L, Bertram, K, Luehrmann, R, Stark, H. | Deposit date: | 2020-02-25 | Release date: | 2020-06-17 | Last modified: | 2020-07-22 | Method: | ELECTRON MICROSCOPY (7.1 Å) | Cite: | Molecular architecture of the human 17S U2 snRNP. Nature, 583, 2020
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6Y50
| 5'domain of human 17S U2 snRNP | Descriptor: | HIV Tat-specific factor 1, PHD finger-like domain-containing protein 5A, Probable ATP-dependent RNA helicase DDX46, ... | Authors: | Zhang, Z, Will, C.L, Bertram, K, Luehrmann, R, Stark, H. | Deposit date: | 2020-02-24 | Release date: | 2020-07-01 | Last modified: | 2020-07-22 | Method: | ELECTRON MICROSCOPY (4.1 Å) | Cite: | Molecular architecture of the human 17S U2 snRNP. Nature, 583, 2020
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5IFE
| Crystal structure of the human SF3b core complex | Descriptor: | PHD finger-like domain-containing protein 5A, POTASSIUM ION, Splicing factor 3B subunit 1, ... | Authors: | Cretu, C, Dybkov, O, De Laurentiis, E, Will, C.L, Luhrmann, R, Pena, V. | Deposit date: | 2016-02-25 | Release date: | 2016-10-26 | Last modified: | 2019-10-16 | Method: | X-RAY DIFFRACTION (3.1 Å) | Cite: | Molecular Architecture of SF3b and Structural Consequences of Its Cancer-Related Mutations. Mol.Cell, 64, 2016
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7OQC
| The U1 part of Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) | Descriptor: | 56 kDa U1 small nuclear ribonucleoprotein component, ACT1 pre-mRNA (delta BS-A),ACT1 pre-mRNA (delta BS-A),ACT1 pre-mRNA (delta BS-A),ACT1 pre-mRNA (delta BS-A),ACT1 pre-mRNA (delta BS-A),ACT1 pre-mRNA (delta BS-A),ACT1 pre-mRNA (delta BS-A),ACT1 pre-mRNA (delta BS-A),ACT1 pre-mRNA (delta BS-A), Pre-mRNA-processing factor 39, ... | Authors: | Zhang, Z, Rigo, N, Dybkov, O, Fourmann, J, Will, C.L, Kumar, V, Urlaub, H, Stark, H, Luehrmann, R. | Deposit date: | 2021-06-03 | Release date: | 2021-08-11 | Last modified: | 2021-09-29 | Method: | ELECTRON MICROSCOPY (4.1 Å) | Cite: | Structural insights into how Prp5 proofreads the pre-mRNA branch site. Nature, 596, 2021
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7OQE
| Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) | Descriptor: | 56 kDa U1 small nuclear ribonucleoprotein component, ACT1 pre-mRNA (delta BS-A), Cold sensitive U2 snRNA suppressor 1, ... | Authors: | Zhang, Z, Rigo, N, Dybkov, O, Fourmann, J, Will, C.L, Kumar, V, Urlaub, H, Stark, H, Luehrmann, R. | Deposit date: | 2021-06-03 | Release date: | 2021-08-11 | Last modified: | 2021-09-29 | Method: | ELECTRON MICROSCOPY (5.9 Å) | Cite: | Structural insights into how Prp5 proofreads the pre-mRNA branch site. Nature, 596, 2021
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7OQB
| The U2 part of Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) | Descriptor: | ACT1 pre-mRNA (delta-BS-A), Cold sensitive U2 snRNA suppressor 1, Pre-mRNA-processing ATP-dependent RNA helicase PRP5, ... | Authors: | Zhang, Z, Rigo, N, Dybkov, O, Fourmann, J, Will, C.L, Kumar, V, Urlaub, H, Stark, H, Luehrmann, R. | Deposit date: | 2021-06-03 | Release date: | 2021-08-11 | Last modified: | 2021-09-22 | Method: | ELECTRON MICROSCOPY (9 Å) | Cite: | Structural insights into how Prp5 proofreads the pre-mRNA branch site. Nature, 596, 2021
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4PJ3
| Structural insight into the function and evolution of the spliceosomal helicase Aquarius, Structure of Aquarius in complex with AMPPNP | Descriptor: | Intron-binding protein aquarius, MAGNESIUM ION, PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | Authors: | De, I, Bessonov, S, Hofele, R, dos Santos, K.F, Will, C.L, Urlaub, H, Luhrmann, R, Pena, V. | Deposit date: | 2014-05-11 | Release date: | 2015-01-21 | Last modified: | 2023-12-27 | Method: | X-RAY DIFFRACTION (2.3 Å) | Cite: | The RNA helicase Aquarius exhibits structural adaptations mediating its recruitment to spliceosomes. Nat.Struct.Mol.Biol., 22, 2015
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5HY7
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7A5P
| Human C Complex Spliceosome - Medium-resolution PERIPHERY | Descriptor: | Cell division cycle 5-like protein, Crooked neck-like protein 1, Eukaryotic initiation factor 4A-III, ... | Authors: | Bertram, K, Kastner, B. | Deposit date: | 2020-08-21 | Release date: | 2020-10-14 | Method: | ELECTRON MICROSCOPY (5 Å) | Cite: | Structural Insights into the Roles of Metazoan-Specific Splicing Factors in the Human Step 1 Spliceosome. Mol.Cell, 80, 2020
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7AAV
| Human pre-Bact-2 spliceosome core structure | Descriptor: | 116 kDa U5 small nuclear ribonucleoprotein component, Cell division cycle 5-like protein, D-chiro inositol hexakisphosphate, ... | Authors: | Townsend, C, Kastner, B, Leelaram, M.N, Bertram, K, Stark, H, Luehrmann, R. | Deposit date: | 2020-09-04 | Release date: | 2020-12-09 | Last modified: | 2020-12-30 | Method: | ELECTRON MICROSCOPY (4.2 Å) | Cite: | Mechanism of protein-guided folding of the active site U2/U6 RNA during spliceosome activation. Science, 370, 2020
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6ZYM
| Human C Complex Spliceosome - High-resolution CORE | Descriptor: | 116 kDa U5 small nuclear ribonucleoprotein component, Cell division cycle 5-like protein, Corepressor interacting with RBPJ 1, ... | Authors: | Bertram, K, Kastner, B. | Deposit date: | 2020-08-02 | Release date: | 2020-10-14 | Method: | ELECTRON MICROSCOPY (3.4 Å) | Cite: | Structural Insights into the Roles of Metazoan-Specific Splicing Factors in the Human Step 1 Spliceosome. Mol.Cell, 80, 2020
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7ABF
| Human pre-Bact-1 spliceosome core structure | Descriptor: | 116 kDa U5 small nuclear ribonucleoprotein component, GUANOSINE-5'-TRIPHOSPHATE, INOSITOL HEXAKISPHOSPHATE, ... | Authors: | Townsend, C, Kastner, B, Leelaram, M.N, Bertram, K, Stark, H, Luehrmann, R. | Deposit date: | 2020-09-07 | Release date: | 2020-12-09 | Last modified: | 2020-12-30 | Method: | ELECTRON MICROSCOPY (3.9 Å) | Cite: | Mechanism of protein-guided folding of the active site U2/U6 RNA during spliceosome activation. Science, 370, 2020
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7ABG
| Human pre-Bact-1 spliceosome | Descriptor: | 116 kDa U5 small nuclear ribonucleoprotein component, 7-METHYL-GUANOSINE-5'-TRIPHOSPHATE-5'-GUANOSINE, Cell division cycle 5-like protein, ... | Authors: | Townsend, C, Kastner, B, Leelaram, M.N, Bertram, K, Stark, H, Luehrmann, R. | Deposit date: | 2020-09-07 | Release date: | 2020-12-23 | Last modified: | 2020-12-30 | Method: | ELECTRON MICROSCOPY (7.8 Å) | Cite: | Mechanism of protein-guided folding of the active site U2/U6 RNA during spliceosome activation. Science, 370, 2020
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7ABH
| Human pre-Bact-2 spliceosome (SF3b/U2 snRNP portion) | Descriptor: | Cell division cycle 5-like protein, DNA/RNA-binding protein KIN17, MINX M3 pre-mRNA, ... | Authors: | Townsend, C, Kastner, B, Leelaram, M.N, Bertram, K, Stark, H, Luehrmann, R. | Deposit date: | 2020-09-07 | Release date: | 2020-12-09 | Last modified: | 2020-12-30 | Method: | ELECTRON MICROSCOPY (4.5 Å) | Cite: | Mechanism of protein-guided folding of the active site U2/U6 RNA during spliceosome activation. Science, 370, 2020
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7ABI
| Human pre-Bact-2 spliceosome | Descriptor: | 116 kDa U5 small nuclear ribonucleoprotein component, BUD13 homolog, Beta-catenin-like protein 1, ... | Authors: | Townsend, C, Kastner, B, Leelaram, M.N, Bertram, K, Stark, H, Luehrmann, R. | Deposit date: | 2020-09-07 | Release date: | 2021-02-10 | Method: | ELECTRON MICROSCOPY (8 Å) | Cite: | Mechanism of protein-guided folding of the active site U2/U6 RNA during spliceosome activation. Science, 370, 2020
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6SLO
| Crystal structure of PUF60 UHM domain in complex with 7,8 dimethoxyperphenazine | Descriptor: | 2-[4-[3-(8-chloranyl-2,3-dimethoxy-phenothiazin-10-yl)propyl]piperazin-1-yl]ethanol, MAGNESIUM ION, Thioredoxin,Poly(U)-binding-splicing factor PUF60 | Authors: | Jagtap, P.K.A, Kubelka, T, Bach, T, Sattler, M. | Deposit date: | 2019-08-20 | Release date: | 2020-09-09 | Last modified: | 2024-01-24 | Method: | X-RAY DIFFRACTION (1.94 Å) | Cite: | Identification of phenothiazine derivatives as UHM-binding inhibitors of early spliceosome assembly. Nat Commun, 11, 2020
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5LSO
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5MQF
| Cryo-EM structure of a human spliceosome activated for step 2 of splicing (C* complex) | Descriptor: | 116 kDa U5 small nuclear ribonucleoprotein component, ATP-dependent RNA helicase DHX8, Cell division cycle 5-like protein, ... | Authors: | Bertram, K, Hartmuth, K, Kastner, B. | Deposit date: | 2016-12-20 | Release date: | 2017-03-22 | Last modified: | 2018-11-21 | Method: | ELECTRON MICROSCOPY (5.9 Å) | Cite: | Cryo-EM structure of a human spliceosome activated for step 2 of splicing. Nature, 542, 2017
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8C6J
| Human spliceosomal PM5 C* complex | Descriptor: | 116 kDa U5 small nuclear ribonucleoprotein component, ADENOSINE-5'-TRIPHOSPHATE, ATP-dependent RNA helicase DHX8, ... | Authors: | Dybkov, O, Kastner, B, Luehrmann, R. | Deposit date: | 2023-01-12 | Release date: | 2023-07-12 | Method: | ELECTRON MICROSCOPY (2.8 Å) | Cite: | Regulation of 3' splice site selection after step 1 of splicing by spliceosomal C* proteins. Sci Adv, 9, 2023
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5O9Z
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6EN4
| SF3b core in complex with a splicing modulator | Descriptor: | PHD finger-like domain-containing protein 5A, Splicing factor 3B subunit 1, Splicing factor 3B subunit 3, ... | Authors: | Cretu, C, Pena, V. | Deposit date: | 2017-10-04 | Release date: | 2018-06-20 | Last modified: | 2024-01-17 | Method: | X-RAY DIFFRACTION (3.08 Å) | Cite: | Structural Basis of Splicing Modulation by Antitumor Macrolide Compounds. Mol. Cell, 70, 2018
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4KIT
| Crystal structure of human Brr2 in complex with the Prp8 Jab1/MPN domain | Descriptor: | ADENOSINE-5'-DIPHOSPHATE, MAGNESIUM ION, Pre-mRNA-processing-splicing factor 8, ... | Authors: | Wahl, M.C, Wandersleben, T, Santos, K.F. | Deposit date: | 2013-05-02 | Release date: | 2013-06-05 | Last modified: | 2023-09-20 | Method: | X-RAY DIFFRACTION (3.598 Å) | Cite: | Inhibition of RNA helicase Brr2 by the C-terminal tail of the spliceosomal protein Prp8. Science, 341, 2013
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