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7JTA
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BU of 7jta by Molmil
Crystal structure of a putative nuclease with anti-Cas9 activity from an uncultured Clostridia bacterium
Descriptor: (4S)-2-METHYL-2,4-PENTANEDIOL, NITRATE ION, NTF2-like nuclease/anti-CRISPR
Authors:Werther, R, Forsberg, K.J, Stoddard, B.L.
Deposit date:2020-08-17
Release date:2021-09-22
Last modified:2022-04-06
Method:X-RAY DIFFRACTION (2.801 Å)
Cite:The novel anti-CRISPR AcrIIA22 relieves DNA torsion in target plasmids and impairs SpyCas9 activity.
Plos Biol., 19, 2021
5T2O
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BU of 5t2o by Molmil
Engineered variant of I-OnuI meganuclease targeting the Anopheles AGAP011377 gene; harbors 53 point mutations relative to wild-type I-OnuI
Descriptor: CALCIUM ION, DNA (26-MER), I-OnuI_e-ag011377
Authors:Stoddard, B.L, Werther, R.
Deposit date:2016-08-23
Release date:2017-05-03
Last modified:2023-10-04
Method:X-RAY DIFFRACTION (2.801 Å)
Cite:Crystallographic analyses illustrate significant plasticity and efficient recoding of meganuclease target specificity.
Nucleic Acids Res., 45, 2017
5T2H
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BU of 5t2h by Molmil
Engineered variant of I-OnuI meganuclease targeting the Human TCRa gene; harbors 43 point mutations relative to wild-type I-OnuI
Descriptor: CALCIUM ION, DNA (26-MER), I-OnuI_e-hTCRa
Authors:Stoddard, B.L, Werther, R.
Deposit date:2016-08-23
Release date:2017-05-03
Last modified:2023-10-04
Method:X-RAY DIFFRACTION (2.517 Å)
Cite:Crystallographic analyses illustrate significant plasticity and efficient recoding of meganuclease target specificity.
Nucleic Acids Res., 45, 2017
5T2N
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BU of 5t2n by Molmil
Engineered variant of I-OnuI meganuclease targeting the Anopheles AGAP007280 gene; harbors 38 point mutations relative to wild-type I-OnuI
Descriptor: CALCIUM ION, DNA (26-MER), I-OnuI_e-ag007820
Authors:Stoddard, B.L, Werther, R.
Deposit date:2016-08-23
Release date:2017-05-03
Last modified:2023-10-04
Method:X-RAY DIFFRACTION (2.079 Å)
Cite:Crystallographic analyses illustrate significant plasticity and efficient recoding of meganuclease target specificity.
Nucleic Acids Res., 45, 2017
5V0Q
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BU of 5v0q by Molmil
Original engineered variant of I-OnuI meganuclease targeting the HIV integrase gene; harbors 49 point mutations relative to wild-type I-OnuI
Descriptor: CALCIUM ION, DNA (26-MER), I-OnuI_e-vHIVInt_v1
Authors:Werther, R, Lambert, A.R.
Deposit date:2017-02-28
Release date:2017-09-27
Last modified:2023-10-04
Method:X-RAY DIFFRACTION (2.4 Å)
Cite:Tuning DNA binding affinity and cleavage specificity of an engineered gene-targeting nuclease via surface display, flow cytometry and cellular analyses.
Protein Eng. Des. Sel., 30, 2017
6UVW
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BU of 6uvw by Molmil
Engineered variant of I-OnuI meganuclease with improved thermostability
Descriptor: CALCIUM ION, DNA (27-MER), I-OnuI-e-Therm
Authors:Werther, R, Stoddard, B.L.
Deposit date:2019-11-04
Release date:2019-12-18
Last modified:2023-10-11
Method:X-RAY DIFFRACTION (2.551 Å)
Cite:Optimization of Protein Thermostability and Exploitation of Recognition Behavior to Engineer Altered Protein-DNA Recognition.
Structure, 28, 2020
6UWK
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BU of 6uwk by Molmil
Engineered variant of I-OnuI meganuclease with improved stability and fully altered specificity targeting human chromosome 11 trans integration site
Descriptor: 1,2-ETHANEDIOL, CALCIUM ION, DNA (27-MER), ...
Authors:Werther, R, Stoddard, B.L.
Deposit date:2019-11-05
Release date:2019-12-18
Last modified:2023-10-11
Method:X-RAY DIFFRACTION (2.533 Å)
Cite:Optimization of Protein Thermostability and Exploitation of Recognition Behavior to Engineer Altered Protein-DNA Recognition.
Structure, 28, 2020
6UWJ
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BU of 6uwj by Molmil
Intermediate engineered variant of I-OnuI meganuclease with improved thermostability and partially altered specificity
Descriptor: CALCIUM ION, DNA (27-MER), I-OnuI-e-Therm-hChr11v2
Authors:Werther, R, Stoddard, B.L.
Deposit date:2019-11-05
Release date:2019-12-18
Last modified:2023-10-11
Method:X-RAY DIFFRACTION (1.852 Å)
Cite:Optimization of Protein Thermostability and Exploitation of Recognition Behavior to Engineer Altered Protein-DNA Recognition.
Structure, 28, 2020
6UWH
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BU of 6uwh by Molmil
Intermediate engineered variant of I-OnuI meganuclease with improved thermostability and partially altered specificity
Descriptor: 1,2-ETHANEDIOL, CALCIUM ION, DNA (26-MER), ...
Authors:Werther, R, Stoddard, B.L.
Deposit date:2019-11-05
Release date:2019-12-18
Last modified:2023-10-11
Method:X-RAY DIFFRACTION (2.299 Å)
Cite:Optimization of Protein Thermostability and Exploitation of Recognition Behavior to Engineer Altered Protein-DNA Recognition.
Structure, 28, 2020
6UW0
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BU of 6uw0 by Molmil
Engineered variant of I-OnuI meganuclease with improved thermostability and fully altered specificity targeting cholera toxin A subunit
Descriptor: 1,2-ETHANEDIOL, CALCIUM ION, DNA (27-MER), ...
Authors:Werther, R, Stoddard, B.L.
Deposit date:2019-11-04
Release date:2019-12-18
Last modified:2023-10-11
Method:X-RAY DIFFRACTION (2.72 Å)
Cite:Optimization of Protein Thermostability and Exploitation of Recognition Behavior to Engineer Altered Protein-DNA Recognition.
Structure, 28, 2020
6UWG
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BU of 6uwg by Molmil
Engineered variant of I-OnuI meganuclease with improved thermostability and E178D mutation at catalytic site
Descriptor: 1,2-ETHANEDIOL, CALCIUM ION, DNA (26-MER), ...
Authors:Werther, R, Stoddard, B.L.
Deposit date:2019-11-05
Release date:2019-12-18
Last modified:2023-10-11
Method:X-RAY DIFFRACTION (2.22 Å)
Cite:Optimization of Protein Thermostability and Exploitation of Recognition Behavior to Engineer Altered Protein-DNA Recognition.
Structure, 28, 2020
5T8D
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BU of 5t8d by Molmil
Engineered variant of I-OnuI meganuclease targeting the HIV integrase gene; harbors 47 point mutations relative to wild-type I-OnuI
Descriptor: CALCIUM ION, DNA (26-MER), I-OnuI_e-vHIVInt_v2
Authors:Stoddard, B.L, Werther, R, Lambert, A.R.
Deposit date:2016-09-07
Release date:2017-05-03
Last modified:2023-10-04
Method:X-RAY DIFFRACTION (2.15 Å)
Cite:Tuning DNA binding affinity and cleavage specificity of an engineered gene-targeting nuclease via surface display, flow cytometry and cellular analyses.
Protein Eng.Des.Sel., 30, 2017
5THG
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BU of 5thg by Molmil
Engineered variant of I-OnuI meganuclease targeting the HIV CCR5 gene; harbors 43 point mutations relative to wild-type I-OnuI
Descriptor: CALCIUM ION, DNA (29-MER), GLYCEROL, ...
Authors:Hallinan, J.P, Stoddard, B.L.
Deposit date:2016-09-29
Release date:2017-05-03
Last modified:2024-03-06
Method:X-RAY DIFFRACTION (3.106 Å)
Cite:The structural basis of altered gene specificity resulting from meganuclease and MegaTAL engineering
to be published
8EMH
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BU of 8emh by Molmil
CryoEM characterization of a unique AAA+ BrxL phage restriction factor
Descriptor: DNA (63-MER), DNA (64-MER), Protease Lon-related BREX system protein BrxL
Authors:Shen, B.W, Stoddard, B.L.
Deposit date:2022-09-27
Release date:2023-02-01
Last modified:2023-05-17
Method:ELECTRON MICROSCOPY (3.63 Å)
Cite:Structure, substrate binding and activity of a unique AAA+ protein: the BrxL phage restriction factor.
Nucleic Acids Res., 51, 2023
8EMC
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BU of 8emc by Molmil
CryoEM characterization of BrxL -- a unique AAA+ phage restriction Factor.
Descriptor: Protease Lon-related BREX system protein BrxL
Authors:Shen, B.W, Stoddard, B.L.
Deposit date:2022-09-27
Release date:2023-02-01
Last modified:2023-05-17
Method:ELECTRON MICROSCOPY (3.6 Å)
Cite:Structure, substrate binding and activity of a unique AAA+ protein: the BrxL phage restriction factor.
Nucleic Acids Res., 51, 2023
8EIL
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BU of 8eil by Molmil
C-Terminal Domain of BrxL from Acinetobacter BREX type I phage restriction system
Descriptor: MALONIC ACID, Protease Lon-related BREX system protein BrxL, SUCCINIC ACID
Authors:Doyle, L.A, Stoddard, B.L, Kaiser, B.
Deposit date:2022-09-15
Release date:2023-02-22
Last modified:2023-05-17
Method:X-RAY DIFFRACTION (2.25 Å)
Cite:Structure, substrate binding and activity of a unique AAA+ protein: the BrxL phage restriction factor.
Nucleic Acids Res., 51, 2023

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PDB entries from 2024-03-27

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