Author results

3J4J
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MODEL OF FULL-LENGTH T. THERMOPHILUS TRANSLATION INITIATION FACTOR 2 REFINED AGAINST ITS CRYO-EM DENSITY FROM A 30S INITIATION COMPLEX MAP
Descriptor:Translation initiation factor IF-2
Authors:Simonetti, A., Marzi, S., Billas, I.M.L., Tsai, A., Fabbretti, A., Myasnikov, A., Roblin, P., Vaiana, A.C., Hazemann, I., Eiler, D., Steitz, T.A., Puglisi, J.D., Gualerzi, C.O., Klaholz, B.P.
Deposit date:2013-08-26
Release date:2013-09-25
Last modified:2018-07-18
Method:ELECTRON MICROSCOPY (11.5 Å)
Cite:Involvement of protein IF2 N domain in ribosomal subunit joining revealed from architecture and function of the full-length initiation factor.
Proc.Natl.Acad.Sci.USA, 110, 2013
4B3X
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BACTERIAL TRANSLATION INITIATION FACTOR IF2 (1-363), APO FORM
Descriptor:TRANSLATION INITIATION FACTOR IF-2, GLYCEROL
Authors:Simonetti, A., Urzhumtsev, A., Klaholz, B.P.
Deposit date:2012-07-26
Release date:2013-05-29
Last modified:2013-06-05
Method:X-RAY DIFFRACTION (1.95 Å)
Cite:Structure of the Protein Core of Translation Initiation Factor 2 in Apo, GTP-Bound and Gdp-Bound Forms
Acta Crystallogr.,Sect.D, 69, 2013
4B43
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BACTERIAL TRANSLATION INITIATION FACTOR IF2 (1-363), APO FORM, DOUBLE MUTANT K86L H130A
Descriptor:TRANSLATION INITIATION FACTOR IF-2, MAGNESIUM ION, GLYCEROL, ...
Authors:Simonetti, A., Urzhumtsev, A., Klaholz, B.P.
Deposit date:2012-07-27
Release date:2013-09-18
Method:X-RAY DIFFRACTION (1.937 Å)
Cite:Molecular Basis of GTP Hydrolysis by Bacterial Translation Initiation Factor If2
To be Published
4B44
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BACTERIAL TRANSLATION INITIATION FACTOR IF2 (1-363), COMPLEX WITH GDP AT PH8.0
Descriptor:TRANSLATION INITIATION FACTOR IF-2, GUANOSINE-5'-DIPHOSPHATE
Authors:Simonetti, A., Urzhumtsev, A., Klaholz, B.P.
Deposit date:2012-07-27
Release date:2013-09-18
Last modified:2015-02-11
Method:X-RAY DIFFRACTION (2.7 Å)
Cite:Molecular Basis of GTP Hydrolysis by Bacterial Translation Initiation Factor If2
To be Published
4B47
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BACTERIAL TRANSLATION INITIATION FACTOR IF2 (1-363), COMPLEX WITH GDP AT PH6.5
Descriptor:TRANSLATION INITIATION FACTOR IF-2, GUANOSINE-5'-DIPHOSPHATE, MAGNESIUM ION
Authors:Simonetti, A., Urzhumtsev, A., Klaholz, B.P.
Deposit date:2012-07-27
Release date:2013-05-29
Last modified:2015-02-11
Method:X-RAY DIFFRACTION (2.301 Å)
Cite:Structure of the Protein Core of Translation Initiation Factor 2 in Apo, GTP-Bound and Gdp-Bound Forms
Acta Crystallogr.,Sect.D, 69, 2013
4B48
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BACTERIAL TRANSLATION INITIATION FACTOR IF2 (1-363), COMPLEX WITH GTP
Descriptor:TRANSLATION INITIATION FACTOR IF-2, GUANOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION
Authors:Simonetti, A., Urzhumtsev, A., Klaholz, B.P.
Deposit date:2012-07-27
Release date:2013-05-29
Last modified:2013-06-05
Method:X-RAY DIFFRACTION (2.8 Å)
Cite:Structure of the Protein Core of Translation Initiation Factor 2 in Apo, GTP-Bound and Gdp-Bound Forms
Acta Crystallogr.,Sect.D, 69, 2013
5K0Y
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M48S LATE-STAGE INITIATION COMPLEX, PURIFIED FROM RABBIT RETICULOCYTES LYSATES, DISPLAYING EIF2 TERNARY COMPLEX AND EIF3 I AND G SUBUNITS RELOCATED TO THE INTERSUBUNIT FACE
Descriptor:tRNA, 18S ribosomal RNA, mRNA, ...
Authors:Simonetti, A., Brito Querido, J., Myasnikov, A.G., Mancera-Martinez, E., Renaud, A., Kuhn, L., Hashem, Y.
Deposit date:2016-05-17
Release date:2016-07-13
Last modified:2018-04-18
Method:ELECTRON MICROSCOPY (5.8 Å)
Cite:eIF3 Peripheral Subunits Rearrangement after mRNA Binding and Start-Codon Recognition.
Mol.Cell, 63, 2016
5K1H
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EIF3B RELOCATED TO THE INTERSUBUNIT FACE TO INTERACT WITH EIF1 AND BELOW THE EIF2 TERNARY-COMPLEX. FROM THE STRUCTURE OF A PARTIAL YEAST 48S PREINITIATION COMPLEX IN CLOSED CONFORMATION.
Descriptor:Eukaryotic translation initiation factor 3 subunit B, eIF3a C-terminal tail
Authors:Simonetti, A., Brito Querido, J., Myasnikov, A.G., Mancera-Martinez, E., Renaud, A., Kuhn, L., Hashem, Y.
Deposit date:2016-05-18
Release date:2016-07-13
Last modified:2018-01-31
Method:ELECTRON MICROSCOPY (4.9 Å)
Cite:eIF3 Peripheral Subunits Rearrangement after mRNA Binding and Start-Codon Recognition.
Mol.Cell, 63, 2016
5ND8
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HIBERNATING RIBOSOME FROM STAPHYLOCOCCUS AUREUS (UNROTATED STATE)
Descriptor:16S ribosomal RNA, 30S ribosomal protein S2, 30S ribosomal protein S3, ...
Authors:Khusainov, I., Vicens, Q., Ayupov, R., Usachev, K., Myasnikov, A., Simonetti, A., Validov, S., Kieffer, B., Yusupova, G., Yusupov, M., Hashem, Y.
Deposit date:2017-03-07
Release date:2017-06-28
Last modified:2019-02-20
Method:ELECTRON MICROSCOPY (3.7 Å)
Cite:Structures and dynamics of hibernating ribosomes from Staphylococcus aureus mediated by intermolecular interactions of HPF.
EMBO J., 36, 2017
5ND9
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HIBERNATING RIBOSOME FROM STAPHYLOCOCCUS AUREUS (ROTATED STATE)
Descriptor:16S ribosomal RNA, 30S ribosomal protein S2, 30S ribosomal protein S3, ...
Authors:Khusainov, I., Vicens, Q., Ayupov, R., Usachev, K., Myasnikov, A., Simonetti, A., Validov, S., Kieffer, B., Yusupova, G., Yusupov, M., Hashem, Y.
Deposit date:2017-03-07
Release date:2017-06-28
Last modified:2019-02-20
Method:ELECTRON MICROSCOPY (3.7 Å)
Cite:Structures and dynamics of hibernating ribosomes from Staphylococcus aureus mediated by intermolecular interactions of HPF.
EMBO J., 36, 2017
5OPT
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STRUCTURE OF KSRP IN CONTEXT OF TRYPANOSOMA CRUZI 40S
Descriptor:Activated protein kinase C receptor, putative, Ribosomal protein S29, ...
Authors:Brito Querido, J., Mancera-Martinez, E., Vicens, Q., Bochler, A., Chicher, J., Simonetti, A., Hashem, Y.
Deposit date:2017-08-10
Release date:2017-11-15
Last modified:2017-12-13
Method:ELECTRON MICROSCOPY (4 Å)
Cite:The cryo-EM Structure of a Novel 40S Kinetoplastid-Specific Ribosomal Protein.
Structure, 25, 2017
5OSG
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STRUCTURE OF KSRP IN CONTEXT OF LEISHMANIA DONOVANI 80S
Descriptor:RNA binding protein, putative, 18S rRNA, ...
Authors:Brito Querido, J., Mancera-Martinez, E., Vicens, Q., Bochler, A., Chicher, J., Simonetti, A., Hashem, Y.
Deposit date:2017-08-17
Release date:2017-11-15
Last modified:2017-12-13
Method:ELECTRON MICROSCOPY (2.9 Å)
Cite:The cryo-EM Structure of a Novel 40S Kinetoplastid-Specific Ribosomal Protein.
Structure, 25, 2017
6GQ1
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CRYO-EM RECONSTRUCTION OF YEAST 80S RIBOSOME IN COMPLEX WITH MRNA, TRNA AND EEF2 (GMPPCP/SORDARIN)
Descriptor:Saccharomyces cerevisiae S288C 35S pre-ribosomal RNA (RDN37-1), miscRNA, 5.8S ribosomal RNA, ...
Authors:Pellegrino, S., Demeshkina, N., Mancera-Martinez, E., Melnikov, S., Simonetti, A., Myasnikov, A., Yusupov, M., Yusupova, G., Hashem, Y.
Deposit date:2018-06-07
Release date:2018-07-11
Last modified:2018-08-08
Method:ELECTRON MICROSCOPY (4.4 Å)
Cite:Structural Insights into the Role of Diphthamide on Elongation Factor 2 in mRNA Reading-Frame Maintenance.
J. Mol. Biol., 430, 2018
5NKO
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SOLUTION STRUCTURE OF THE C-TERMINAL DOMAIN OF S. AUREUS HIBERNATING PROMOTING FACTOR (CTD-SAHPF)
Descriptor:Ribosome hibernation promotion factor
Authors:Usachev, K.S., Khusainov, I.S., Ayupov, R.K., Validov, S.Z., Kieffer, B., Yusupov, M.M.
Deposit date:2017-03-31
Release date:2017-07-05
Last modified:2017-07-26
Method:SOLUTION NMR
Cite:Structures and dynamics of hibernating ribosomes from Staphylococcus aureus mediated by intermolecular interactions of HPF.
EMBO J., 36, 2017
6GQB
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CRYO-EM RECONSTRUCTION OF YEAST 80S RIBOSOME IN COMPLEX WITH MRNA, TRNA AND EEF2 (GDP+ALF4/SORDARIN)
Descriptor:Saccharomyces cerevisiae S288C 35S pre-ribosomal RNA (RDN37-1), miscRNA, 5S ribosomal RNA, ...
Authors:Pellegrino, S., Yusupov, M., Yusupova, G., Hashem, Y.
Deposit date:2018-06-07
Release date:2018-07-11
Last modified:2018-11-28
Method:ELECTRON MICROSCOPY (3.9 Å)
Cite:Structural Insights into the Role of Diphthamide on Elongation Factor 2 in mRNA Reading-Frame Maintenance.
J. Mol. Biol., 430, 2018
6GQV
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CRYO-EM RECOSNTRUCTION OF YEAST 80S RIBOSOME IN COMPLEX WITH MRNA, TRNA AND EEF2 (GMPPCP)
Descriptor:25S ribosomal RNA, 5S ribosomal RNA, 5.8S ribosomal RNA, ...
Authors:Pellegrino, S., Yusupov, M., Yusupova, G., Hashem, Y.
Deposit date:2018-06-08
Release date:2018-07-11
Last modified:2018-08-08
Method:ELECTRON MICROSCOPY (4 Å)
Cite:Structural Insights into the Role of Diphthamide on Elongation Factor 2 in mRNA Reading-Frame Maintenance.
J. Mol. Biol., 430, 2018
4KJZ
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CRYSTAL STRUCTURE OF THERMUS THERMOPHILUS IF2, APO AND GDP-BOUND FORMS (2-474)
Descriptor:Translation initiation factor IF-2, GUANOSINE-5'-DIPHOSPHATE
Authors:Eiler, D.R., Lin, J., Steitz, T.A.
Deposit date:2013-05-04
Release date:2013-09-11
Last modified:2013-10-09
Method:X-RAY DIFFRACTION (2.8 Å)
Cite:Initiation factor 2 crystal structure reveals a different domain organization from eukaryotic initiation factor 5B and mechanism among translational GTPases.
Proc.Natl.Acad.Sci.USA, 110, 2013