7P38
 
 | | 4,6-alpha-glucanotransferase GtfB from Limosilactobacillus reuteri NCC 2613 | | Descriptor: | CALCIUM ION, Dextransucrase, GLYCEROL, ... | | Authors: | Pijning, T, te Poele, E, Gangoiti, J, Boerner, T, Dijkhuizen, L. | | Deposit date: | 2021-07-07 | | Release date: | 2021-11-03 | | Last modified: | 2021-11-17 | | Method: | X-RAY DIFFRACTION (2.7 Å) | | Cite: | Insights into Broad-Specificity Starch Modification from the Crystal Structure of Limosilactobacillus Reuteri NCC 2613 4,6-alpha-Glucanotransferase GtfB.
J.Agric.Food Chem., 69, 2021
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7P39
 | | 4,6-alpha-glucanotransferase GtfB from Limosilactobacillus reuteri NCC 2613 complexed with acarbose | | Descriptor: | 4,6-dideoxy-4-{[(1S,4R,5S,6S)-4,5,6-trihydroxy-3-(hydroxymethyl)cyclohex-2-en-1-yl]amino}-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-beta-D-glucopyranose, CALCIUM ION, Dextransucrase, ... | | Authors: | Pijning, T, te Poele, E, Gangoiti, J, Boerner, T, Dijkhuizen, L. | | Deposit date: | 2021-07-07 | | Release date: | 2021-11-03 | | Last modified: | 2021-11-17 | | Method: | X-RAY DIFFRACTION (2.9 Å) | | Cite: | Insights into Broad-Specificity Starch Modification from the Crystal Structure of Limosilactobacillus Reuteri NCC 2613 4,6-alpha-Glucanotransferase GtfB.
J.Agric.Food Chem., 69, 2021
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7ZC0
 | | 4,6-alpha-glucanotransferase GtfC from Geobacillus 12AMOR1 | | Descriptor: | 4,6-alpha-Glucanotransferase, CALCIUM ION, GLYCEROL | | Authors: | Pijning, T, Dijkhuizen, L, Guskov, A, te Poele, E.M. | | Deposit date: | 2022-03-25 | | Release date: | 2022-12-07 | | Last modified: | 2024-01-31 | | Method: | X-RAY DIFFRACTION (2.25 Å) | | Cite: | Crystal Structure of 4,6-alpha-Glucanotransferase GtfC-Delta C from Thermophilic Geobacillus 12AMOR1: Starch Transglycosylation in Non-Permuted GH70 Enzymes.
J.Agric.Food Chem., 70, 2022
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8B57
 | | Structure of prolyl endoprotease from Aspergillus niger CBS 109712 | | Descriptor: | 2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, DI(HYDROXYETHYL)ETHER, ... | | Authors: | Pijning, T, Vujicic-Zagar, A, Van der Laan, J.M, De Jong, R.M, Dijkstra, B.W. | | Deposit date: | 2022-09-22 | | Release date: | 2023-12-20 | | Last modified: | 2024-01-10 | | Method: | X-RAY DIFFRACTION (2.42 Å) | | Cite: | Structural and time-resolved mechanistic investigations of protein hydrolysis by the acidic proline-specific endoprotease from Aspergillus niger.
Protein Sci., 33, 2024
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8BBX
 | | Structure of prolyl endoprotease from Aspergillus niger CBS 109712 in space group C222(1) | | Descriptor: | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, Endoprotease endo-Pro, TETRAETHYLENE GLYCOL, ... | | Authors: | Pijning, T, Vujicic-Zagar, A, Van der Laan, J.M, De Jong, R.M, Dijkstra, B.W. | | Deposit date: | 2022-10-14 | | Release date: | 2023-12-20 | | Last modified: | 2024-01-10 | | Method: | X-RAY DIFFRACTION (2.7 Å) | | Cite: | Structural and time-resolved mechanistic investigations of protein hydrolysis by the acidic proline-specific endoprotease from Aspergillus niger.
Protein Sci., 33, 2024
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4AMC
 | | Crystal structure of Lactobacillus reuteri 121 N-terminally truncated glucansucrase GTFA | | Descriptor: | CALCIUM ION, GLUCANSUCRASE | | Authors: | Pijning, T, Vujicic-Zagar, A, Kralj, S, Dijkhuizen, L, Dijkstra, B.W. | | Deposit date: | 2012-03-08 | | Release date: | 2012-11-07 | | Last modified: | 2023-12-20 | | Method: | X-RAY DIFFRACTION (3.6 Å) | | Cite: | Structure of the Alpha-1,6/Alpha-1,4-Specific Glucansucrase Gtfa from Lactobacillus Reuteri 121
Acta Crystallogr.,Sect.F, 68, 2012
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4AYG
 | | Lactobacillus reuteri N-terminally truncated glucansucrase GTF180 in orthorhombic apo-form | | Descriptor: | ACETIC ACID, CALCIUM ION, GLUCANSUCRASE, ... | | Authors: | Pijning, T, Vujicic-Zagar, A, Kralj, S, Dijkhuizen, L, Dijkstra, B.W. | | Deposit date: | 2012-06-21 | | Release date: | 2013-07-03 | | Last modified: | 2023-12-20 | | Method: | X-RAY DIFFRACTION (2 Å) | | Cite: | Flexibility of Truncated and Full-Length Glucansucrase Gtf180 Enzymes from Lactobacillus Reuteri 180.
FEBS J., 281, 2014
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3JUR
 | | The crystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima | | Descriptor: | Exo-poly-alpha-D-galacturonosidase | | Authors: | Pijning, T, van Pouderoyen, G, Kluskens, L.D, van der Oost, J, Dijkstra, B.W. | | Deposit date: | 2009-09-15 | | Release date: | 2009-11-17 | | Last modified: | 2023-11-01 | | Method: | X-RAY DIFFRACTION (2.05 Å) | | Cite: | The crystal structure of a hyperthermoactive exopolygalacturonase from Thermotoga maritima reveals a unique tetramer
Febs Lett., 2009
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2YFT
 | | Crystal structure of inulosucrase from Lactobacillus johnsonii NCC533 in complex with 1-kestose | | Descriptor: | ACETATE ION, CALCIUM ION, LEVANSUCRASE, ... | | Authors: | Pijning, T, Anwar, M.A, Leemhuis, H, Kralj, S, Dijkhuizen, L, Dijkstra, B.W. | | Deposit date: | 2011-04-07 | | Release date: | 2011-08-03 | | Last modified: | 2023-12-20 | | Method: | X-RAY DIFFRACTION (1.85 Å) | | Cite: | Crystal Structure of Inulosucrase from Lactobacillus: Insights Into the Substrate Specificity and Product Specificity of Gh68 Fructansucrases.
J.Mol.Biol., 412, 2011
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2YFS
 | | Crystal structure of inulosucrase from Lactobacillus johnsonii NCC533 in complex with sucrose | | Descriptor: | CALCIUM ION, LEVANSUCRASE, SULFATE ION, ... | | Authors: | Pijning, T, Anwar, M.A, Leemhuis, H, Kralj, S, Dijkhuizen, L, Dijkstra, B.W. | | Deposit date: | 2011-04-07 | | Release date: | 2011-08-03 | | Last modified: | 2023-12-20 | | Method: | X-RAY DIFFRACTION (2.6 Å) | | Cite: | Crystal Structure of Inulosucrase from Lactobacillus: Insights Into the Substrate Specificity and Product Specificity of Gh68 Fructansucrases.
J.Mol.Biol., 412, 2011
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2YFR
 | | Crystal structure of inulosucrase from Lactobacillus johnsonii NCC533 | | Descriptor: | ACETATE ION, CALCIUM ION, CHLORIDE ION, ... | | Authors: | Pijning, T, Anwar, M.A, Leemhuis, H, Kralj, S, Dijkhuizen, L, Dijkstra, B.W. | | Deposit date: | 2011-04-07 | | Release date: | 2011-08-03 | | Last modified: | 2023-12-20 | | Method: | X-RAY DIFFRACTION (1.75 Å) | | Cite: | Crystal Structure of Inulosucrase from Lactobacillus: Insights Into the Substrate Specificity and Product Specificity of Gh68 Fructansucrases.
J.Mol.Biol., 412, 2011
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6FPS
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3P0B
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5JBF
 | | 4,6-alpha-glucanotransferase GTFB (D1015N mutant) from Lactobacillus reuteri 121 complexed with maltopentaose | | Descriptor: | CALCIUM ION, Inactive glucansucrase, SULFATE ION, ... | | Authors: | Pijning, T, Dijkstra, B.W, Bai, Y, Gangoiti-Munecas, J, Dijkhuizen, L. | | Deposit date: | 2016-04-13 | | Release date: | 2017-01-18 | | Last modified: | 2024-01-10 | | Method: | X-RAY DIFFRACTION (2.19 Å) | | Cite: | Crystal Structure of 4,6-alpha-Glucanotransferase Supports Diet-Driven Evolution of GH70 Enzymes from alpha-Amylases in Oral Bacteria.
Structure, 25, 2017
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5JBD
 | | 4,6-alpha-glucanotransferase GTFB from Lactobacillus reuteri 121 | | Descriptor: | ACETATE ION, CALCIUM ION, GLYCEROL, ... | | Authors: | Pijning, T, Dijkstra, B.W, Bai, Y, Gangoiti-Munecas, J, Dijkhuizen, L. | | Deposit date: | 2016-04-13 | | Release date: | 2017-01-18 | | Last modified: | 2024-01-10 | | Method: | X-RAY DIFFRACTION (1.8 Å) | | Cite: | Crystal Structure of 4,6-alpha-Glucanotransferase Supports Diet-Driven Evolution of GH70 Enzymes from alpha-Amylases in Oral Bacteria.
Structure, 25, 2017
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5JBE
 | | 4,6-alpha-glucanotransferase GTFB from Lactobacillus reuteri 121 complexed with an isomalto-maltopentasaccharide | | Descriptor: | ACETATE ION, CALCIUM ION, Inactive glucansucrase, ... | | Authors: | Pijning, T, Dijkstra, B.W, Bai, Y, Gangoiti-Munecas, J, Dijkhuizen, L. | | Deposit date: | 2016-04-13 | | Release date: | 2017-01-18 | | Last modified: | 2024-01-10 | | Method: | X-RAY DIFFRACTION (2.1 Å) | | Cite: | Crystal Structure of 4,6-alpha-Glucanotransferase Supports Diet-Driven Evolution of GH70 Enzymes from alpha-Amylases in Oral Bacteria.
Structure, 25, 2017
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3QZU
 | | Crystal structure of Bacillus subtilis Lipase A 7-fold mutant; the outcome of directed evolution towards thermostability | | Descriptor: | CHLORIDE ION, GLYCEROL, Lipase estA, ... | | Authors: | Pijning, T, Augustyniak, W, Reetz, M.T, Dijkstra, B.W. | | Deposit date: | 2011-03-07 | | Release date: | 2012-02-08 | | Last modified: | 2023-09-13 | | Method: | X-RAY DIFFRACTION (1.85 Å) | | Cite: | Biophysical characterization of mutants of Bacillus subtilis lipase evolved for thermostability: Factors contributing to increased activity retention.
Protein Sci., 21, 2012
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8OI4
 | | Metagenomic Beta-galactosidase from Glycoside Hydrolase family GH154 | | Descriptor: | Beta-galactosidase, CHLORIDE ION, GLYCEROL | | Authors: | Pijning, T, Hameleers, L, Jurak, E, Guskov, A. | | Deposit date: | 2023-03-22 | | Release date: | 2024-01-31 | | Method: | X-RAY DIFFRACTION (1.76 Å) | | Cite: | Novel beta-galactosidase activity and first crystal structure of Glycoside Hydrolase family 154.
N Biotechnol, 80, 2023
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5MYJ
 | | Structure of 70S ribosome from Lactococcus lactis | | Descriptor: | 16S ribosomal RNA, 23S ribosomal RNA, 30S ribosomal protein S10, ... | | Authors: | Franken, L.E, Oostergetel, G.T, Pijning, T, Puri, P, Boekema, E.J, Poolman, B, Guskov, A. | | Deposit date: | 2017-01-26 | | Release date: | 2017-10-11 | | Method: | ELECTRON MICROSCOPY (5.6 Å) | | Cite: | A general mechanism of ribosome dimerization revealed by single-particle cryo-electron microscopy.
Nat Commun, 8, 2017
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1A6J
 | | NITROGEN REGULATORY BACTERIAL PROTEIN IIA-NITROGEN | | Descriptor: | BETA-MERCAPTOETHANOL, NITROGEN REGULATORY IIA PROTEIN, SULFATE ION | | Authors: | Bordo, D, Van Montfort, R, Pijning, T, Kalk, K.H, Reizer, J, Saier, M.H, Dijkstra, B.W. | | Deposit date: | 1998-02-25 | | Release date: | 1998-07-15 | | Last modified: | 2024-02-07 | | Method: | X-RAY DIFFRACTION (2.35 Å) | | Cite: | The three-dimensional structure of the nitrogen regulatory protein IIANtr from Escherichia coli.
J.Mol.Biol., 279, 1998
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1A3A
 | | CRYSTAL STRUCTURE OF IIA MANNITOL FROM ESCHERICHIA COLI | | Descriptor: | MANNITOL-SPECIFIC EII | | Authors: | Van Montfort, R.L.M, Pijning, T, Kalk, K.H, Hangyi, I, Kouwijzer, M.L.C.E, Robillard, G.T, Dijkstra, B.W. | | Deposit date: | 1998-01-19 | | Release date: | 1998-08-12 | | Last modified: | 2024-02-07 | | Method: | X-RAY DIFFRACTION (1.8 Å) | | Cite: | The structure of the Escherichia coli phosphotransferase IIAmannitol reveals a novel fold with two conformations of the active site.
Structure, 6, 1998
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7FDZ
 | | Levansucrase from Brenneria sp. EniD 312 with sucrose | | Descriptor: | 3,6,9,12,15,18,21-HEPTAOXATRICOSANE-1,23-DIOL, DI(HYDROXYETHYL)ETHER, GLYCEROL, ... | | Authors: | Xu, W, Hou, X.D, Rao, Y.J, Pijning, T, Guskov, A, Mu, W.M. | | Deposit date: | 2021-07-19 | | Release date: | 2022-04-20 | | Last modified: | 2023-11-29 | | Method: | X-RAY DIFFRACTION (1.35 Å) | | Cite: | Crystal Structure of Levansucrase from the Gram-Negative Bacterium Brenneria Provides Insights into Its Product Size Specificity.
J.Agric.Food Chem., 70, 2022
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1NWR
 | | Crystal structure of human cartilage gp39 (HC-gp39) | | Descriptor: | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, Chitinase-3 like protein 1 | | Authors: | Fusetti, F, Pijning, T, Kalk, K.H, Bos, E, Dijkstra, B.W. | | Deposit date: | 2003-02-06 | | Release date: | 2003-08-26 | | Last modified: | 2020-07-29 | | Method: | X-RAY DIFFRACTION (2.7 Å) | | Cite: | Crystal Structure and Carbohydrate-binding Properties of the Human Cartilage Glycoprotein-39
J.Biol.Chem., 278, 2003
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1NWT
 | | Crystal structure of human cartilage gp39 (HC-gp39) in complex with chitopentaose | | Descriptor: | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-alpha-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-alpha-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-alpha-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... | | Authors: | Fusetti, F, Pijning, T, Kalk, K.H, Bos, E, Dijkstra, B.W. | | Deposit date: | 2003-02-06 | | Release date: | 2003-08-26 | | Last modified: | 2020-07-29 | | Method: | X-RAY DIFFRACTION (2.5 Å) | | Cite: | Crystal structure and carbohydrate-binding properties of the human cartilage glycoprotein-39
J.Biol.Chem., 278, 2003
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1YOB
 | | C69A Flavodoxin II from Azotobacter vinelandii | | Descriptor: | FLAVIN MONONUCLEOTIDE, Flavodoxin 2, SULFATE ION | | Authors: | Alagaratnam, S, van Pouderoyen, G, Pijning, T, Dijkstra, B.W, Cavazzini, D, Rossi, G.L, Canters, G.W. | | Deposit date: | 2005-01-27 | | Release date: | 2005-10-18 | | Last modified: | 2023-10-25 | | Method: | X-RAY DIFFRACTION (2.25 Å) | | Cite: | A crystallographic study of Cys69Ala flavodoxin II from Azotobacter vinelandii: structural determinants of redox potential
Protein Sci., 14, 2005
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