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1ACM
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ARGININE 54 IN THE ACTIVE SITE OF ESCHERICHIA COLI ASPARTATE TRANSCARBAMOYLASE IS CRITICAL FOR CATALYSIS: A SITE-SPECIFIC MUTAGENESIS, NMR AND X-RAY CRYSTALLOGRAPHY STUDY
Descriptor:ASPARTATE CARBAMOYLTRANSFERASE, CATALYTIC CHAIN, ASPARTATE CARBAMOYLTRANSFERASE REGULATORY CHAIN, ...
Authors:Stevens, R.C., Kantrowitz, E.R., Lipscomb, W.N.
Deposit date:1992-07-08
Release date:1992-07-15
Last modified:2017-11-29
Method:X-RAY DIFFRACTION (2.8 Å)
Cite:Arginine 54 in the active site of Escherichia coli aspartate transcarbamoylase is critical for catalysis: a site-specific mutagenesis, NMR, and X-ray crystallographic study.
Protein Sci., 1, 1992
1ANI
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ALKALINE PHOSPHATASE (D153H, K328H)
Descriptor:ALKALINE PHOSPHATASE, ZINC ION, PHOSPHATE ION
Authors:Murphy, J.E., Tibbitts, T.T., Kantrowitz, E.R.
Deposit date:1995-09-06
Release date:1996-01-29
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.5 Å)
Cite:Mutations at positions 153 and 328 in Escherichia coli alkaline phosphatase provide insight towards the structure and function of mammalian and yeast alkaline phosphatases.
J.Mol.Biol., 253, 1995
1ANJ
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ALKALINE PHOSPHATASE (K328H)
Descriptor:ALKALINE PHOSPHATASE, ZINC ION, PHOSPHATE ION
Authors:Murphy, J.E., Tibbitts, T.T., Kantrowitz, E.R.
Deposit date:1995-09-06
Release date:1996-01-29
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.3 Å)
Cite:Mutations at positions 153 and 328 in Escherichia coli alkaline phosphatase provide insight towards the structure and function of mammalian and yeast alkaline phosphatases.
J.Mol.Biol., 253, 1995
1HJK
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ALKALINE PHOSPHATASE MUTANT H331Q
Descriptor:ALKALINE PHOSPHATASE, ZINC ION, MAGNESIUM ION, ...
Authors:Murphy, J.E., Stec, B., Ma, L., Kantrowitz, E.R.
Deposit date:1997-05-30
Release date:1997-10-15
Last modified:2011-11-16
Method:X-RAY DIFFRACTION (2.3 Å)
Cite:Trapping and visualization of a covalent enzyme-phosphate intermediate.
Nat.Struct.Biol., 4, 1997
1HQA
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ALKALINE PHOSPHATASE (H412Q)
Descriptor:ALKALINE PHOSPHATASE, ZINC ION
Authors:Ma, L., Kantrowitz, E.R.
Deposit date:1995-11-30
Release date:1996-03-08
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.25 Å)
Cite:Kinetic and X-ray structural studies of a mutant Escherichia coli alkaline phosphatase (His-412-->Gln) at one of the zinc binding sites.
Biochemistry, 35, 1996
1URA
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ALKALINE PHOSPHATASE (D51ZN)
Descriptor:ALKALINE PHOSPHATASE, ZINC ION, PHOSPHATE ION
Authors:Tibbitts, T.T., Murphy, J.E., Kantrowitz, E.R.
Deposit date:1996-02-03
Release date:1996-07-11
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.04 Å)
Cite:Kinetic and structural consequences of replacing the aspartate bridge by asparagine in the catalytic metal triad of Escherichia coli alkaline phosphatase.
J.Mol.Biol., 257, 1996
1URB
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ALKALINE PHOSPHATASE (N51MG)
Descriptor:ALKALINE PHOSPHATASE, ZINC ION, MAGNESIUM ION, ...
Authors:Tibbitts, T.T., Murphy, J.E., Kantrowitz, E.R.
Deposit date:1996-02-03
Release date:1996-07-11
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.14 Å)
Cite:Kinetic and structural consequences of replacing the aspartate bridge by asparagine in the catalytic metal triad of Escherichia coli alkaline phosphatase.
J.Mol.Biol., 257, 1996
1ALI
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ALKALINE PHOSPHATASE MUTANT (H412N)
Descriptor:ALKALINE PHOSPHATASE, ZINC ION, MAGNESIUM ION, ...
Authors:Ma, L., Tibbitts, T.T., Kantrowitz, E.R.
Deposit date:1995-06-02
Release date:1995-11-14
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.2 Å)
Cite:Escherichia coli alkaline phosphatase: X-ray structural studies of a mutant enzyme (His-412-->Asn) at one of the catalytically important zinc binding sites.
Protein Sci., 4, 1995
1ALJ
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ALKALINE PHOSPHATASE MUTANT (H412N)
Descriptor:ALKALINE PHOSPHATASE, ZINC ION, MAGNESIUM ION, ...
Authors:Ma, L., Tibbitts, T.T., Kantrowitz, E.R.
Deposit date:1995-06-02
Release date:1995-11-14
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.6 Å)
Cite:Escherichia coli alkaline phosphatase: X-ray structural studies of a mutant enzyme (His-412-->Asn) at one of the catalytically important zinc binding sites.
Protein Sci., 4, 1995
1B8J
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ALKALINE PHOSPHATASE COMPLEXED WITH VANADATE
Descriptor:PROTEIN (ALKALINE PHOSPHATASE), ZINC ION, SULFATE ION, ...
Authors:Holtz, K.M., Stec, B., Kantrowitz, E.R.
Deposit date:1999-02-01
Release date:1999-02-18
Last modified:2017-10-04
Method:X-RAY DIFFRACTION (1.9 Å)
Cite:A model of the transition state in the alkaline phosphatase reaction.
J.Biol.Chem., 274, 1999
1EW8
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ALKALINE PHOSPHATASE (E.C. 3.1.3.1) COMPLEX WITH PHOSPHONOACETIC ACID
Descriptor:ALKALINE PHOSPHATASE, ZINC ION, PHOSPHATE ION, ...
Authors:Holtz, K.M., Stec, B., Myers, J.K., Antonelli, S.M., Widlanski, T.S., Kantrowitz, E.R.
Deposit date:2000-04-24
Release date:2002-05-01
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.2 Å)
Cite:Alternate modes of binding in two crystal structures of alkaline phosphatase-inhibitor complexes.
Protein Sci., 9, 2000
1EW9
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ALKALINE PHOSPHATASE (E.C. 3.1.3.1) COMPLEX WITH MERCAPTOMETHYL PHOSPHONATE
Descriptor:ALKALINE PHOSPHATASE, ZINC ION, SULFATE ION, ...
Authors:Holtz, K.M., Stec, B., Meyers, J.K., Antonelli, S.M., Widlanski, T.S., Kantrowitz, E.R.
Deposit date:2000-04-24
Release date:2002-05-01
Last modified:2018-01-31
Method:X-RAY DIFFRACTION (2 Å)
Cite:Alternate modes of binding in two crystal structures of alkaline phosphatase-inhibitor complexes.
Protein Sci., 9, 2000
1F1B
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CRYSTAL STRUCTURE OF E. COLI ASPARTATE TRANSCARBAMOYLASE P268A MUTANT IN THE R-STATE IN THE PRESENCE OF N-PHOSPHONACETYL-L-ASPARTATE
Descriptor:ASPARTATE CARBAMOYLTRANSFERASE CATALYTIC CHAIN, ASPARTATE CARBAMOYLTRANSFERASE REGULATORY CHAIN, N-(PHOSPHONACETYL)-L-ASPARTIC ACID, ...
Authors:Jin, L., Stec, B., Kantrowitz, E.R.
Deposit date:2000-05-18
Release date:2000-11-22
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.3 Å)
Cite:A cis-proline to alanine mutant of E. coli aspartate transcarbamoylase: kinetic studies and three-dimensional crystal structures.
Biochemistry, 39, 2000
1FSA
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THE T-STATE STRUCTURE OF LYS 42 TO ALA MUTANT OF THE PIG KIDNEY FRUCTOSE 1,6-BISPHOSPHATASE EXPRESSED IN E. COLI
Descriptor:FRUCTOSE 1,6-BISPHOSPHATASE, FRUCTOSE-6-PHOSPHATE, MANGANESE (II) ION, ...
Authors:Lu, G., Stec, B., Giroux, E., Kantrowitz, E.R.
Deposit date:1996-08-24
Release date:1997-09-04
Last modified:2011-11-16
Method:X-RAY DIFFRACTION (2.3 Å)
Cite:Evidence for an active T-state pig kidney fructose 1,6-bisphosphatase: interface residue Lys-42 is important for allosteric inhibition and AMP cooperativity.
Protein Sci., 5, 1996
2ANH
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ALKALINE PHOSPHATASE (D153H)
Descriptor:ALKALINE PHOSPHATASE, ZINC ION, PHOSPHATE ION
Authors:Murphy, J.E., Tibbitts, T.T., Kantrowitz, E.R.
Deposit date:1995-09-06
Release date:1996-01-29
Last modified:2011-11-16
Method:X-RAY DIFFRACTION (2.4 Å)
Cite:Mutations at positions 153 and 328 in Escherichia coli alkaline phosphatase provide insight towards the structure and function of mammalian and yeast alkaline phosphatases.
J.Mol.Biol., 253, 1995
1ALH
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KINETICS AND CRYSTAL STRUCTURE OF A MUTANT E. COLI ALKALINE PHOSPHATASE (ASP-369-->ASN): A MECHANISM INVOLVING ONE ZINC PER ACTIVE SITE
Descriptor:ALKALINE PHOSPHATASE, ZINC ION, PHOSPHATE ION, ...
Authors:Tibbitts, T.T., Xu, X., Kantrowitz, E.R.
Deposit date:1994-08-23
Release date:1995-02-27
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.5 Å)
Cite:Kinetics and crystal structure of a mutant Escherichia coli alkaline phosphatase (Asp-369-->Asn): a mechanism involving one zinc per active site.
Protein Sci., 3, 1994
1D09
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ASPARTATE TRANSCARBAMOYLASE COMPLEXED WITH N-PHOSPHONACETYL-L-ASPARTATE (PALA)
Descriptor:ASPARTATE CARBAMOYLTRANSFERASE CATALYTIC CHAIN, ASPARTATE CARBAMOYLTRANSFERASE REGULATORY CHAIN, N-(PHOSPHONACETYL)-L-ASPARTIC ACID, ...
Authors:Jin, L., Stec, B., Lipscomb, W.N., Kantrowitz, E.R.
Deposit date:1999-09-09
Release date:2000-01-28
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.1 Å)
Cite:Insights into the mechanisms of catalysis and heterotropic regulation of Escherichia coli aspartate transcarbamoylase based upon a structure of the enzyme complexed with the bisubstrate analogue N-phosphonacetyl-L-aspartate at 2.1 A.
Proteins, 37, 1999
1ED8
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STRUCTURE OF E. COLI ALKALINE PHOSPHATASE INHIBITED BY THE INORGANIC PHOSPHATE AT 1.75A RESOLUTION
Descriptor:ALKALINE PHOSPHATASE, ZINC ION, MAGNESIUM ION, ...
Authors:Stec, B., Holtz, K.M., Kantrowitz, E.R.
Deposit date:2000-01-27
Release date:2000-09-20
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.75 Å)
Cite:A revised mechanism for the alkaline phosphatase reaction involving three metal ions.
J.Mol.Biol., 299, 2000
1ED9
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STRUCTURE OF E. COLI ALKALINE PHOSPHATASE WITHOUT THE INORGANIC PHOSPHATE AT 1.75A RESOLUTION
Descriptor:ALKALINE PHOSPHATASE, ZINC ION, MAGNESIUM ION, ...
Authors:Stec, B., Holtz, K.M., Kantrowitz, E.R.
Deposit date:2000-01-27
Release date:2000-09-20
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.75 Å)
Cite:A revised mechanism for the alkaline phosphatase reaction involving three metal ions.
J.Mol.Biol., 299, 2000
1ELX
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E. COLI ALKALINE PHOSPHATASE MUTANT (S102A)
Descriptor:ALKALINE PHOSPHATASE, ZINC ION, MAGNESIUM ION, ...
Authors:Stec, B., Hehir, M., Brennan, C., Nolte, M., Kantrowitz, E.R.
Deposit date:1998-02-10
Release date:1998-05-27
Last modified:2011-11-16
Method:X-RAY DIFFRACTION (2.6 Å)
Cite:Kinetic and X-ray structural studies of three mutant E. coli alkaline phosphatases: insights into the catalytic mechanism without the nucleophile Ser102.
J.Mol.Biol., 277, 1998
1ELY
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E. COLI ALKALINE PHOSPHATASE MUTANT (S102C)
Descriptor:ALKALINE PHOSPHATASE, ZINC ION, MAGNESIUM ION, ...
Authors:Stec, B., Hehir, M., Brennan, C., Nolte, M., Kantrowitz, E.R.
Deposit date:1998-02-10
Release date:1998-05-27
Last modified:2011-11-16
Method:X-RAY DIFFRACTION (2.8 Å)
Cite:Kinetic and X-ray structural studies of three mutant E. coli alkaline phosphatases: insights into the catalytic mechanism without the nucleophile Ser102.
J.Mol.Biol., 277, 1998
1ELZ
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E. COLI ALKALINE PHOSPHATASE MUTANT (S102G)
Descriptor:ALKALINE PHOSPHATASE, ZINC ION, MAGNESIUM ION, ...
Authors:Stec, B., Hehir, M., Brennan, C., Nolte, M., Kantrowitz, E.R.
Deposit date:1998-02-10
Release date:1998-05-27
Last modified:2011-11-16
Method:X-RAY DIFFRACTION (2.8 Å)
Cite:Kinetic and X-ray structural studies of three mutant E. coli alkaline phosphatases: insights into the catalytic mechanism without the nucleophile Ser102.
J.Mol.Biol., 277, 1998
1EZZ
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CRYSTAL STRUCTURE OF E. COLI ASPARTATE TRANSCARBAMOYLASE P268A MUTANT IN THE T-STATE
Descriptor:ASPARTATE CARBAMOYLTRANSFERASE CATALYTIC CHAIN, ASPARTATE CARBAMOYLTRANSFERASE REGULATORY CHAIN, ZINC ION
Authors:Jin, L., Stec, B., Kantrowitz, E.R.
Deposit date:2000-05-12
Release date:2000-11-22
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.7 Å)
Cite:A cis-proline to alanine mutant of E. coli aspartate transcarbamoylase: kinetic studies and three-dimensional crystal structures.
Biochemistry, 39, 2000
1I5O
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CRYSTAL STRUCTURE OF MUTANT R105A OF E. COLI ASPARTATE TRANSCARBAMOYLASE
Descriptor:ASPARTATE TRANSCARBAMOYLASE CATALYTIC CHAIN, ASPARTATE TRANSCARBAMOYLASE REGULATORY CHAIN, ZINC ION, ...
Authors:Macol, C.P., Tsuruta, H., Stec, B., Kantrowitz, E.R.
Deposit date:2001-02-28
Release date:2001-05-02
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.8 Å)
Cite:Direct structural evidence for a concerted allosteric transition in Escherichia coli aspartate transcarbamoylase.
Nat.Struct.Biol., 8, 2001
1NBE
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ASPARTATE TRANSCARBAMOYLASE REGULATORY CHAIN MUTANT (T82A)
Descriptor:ASPARTATE TRANSCARBAMOYLASE, D-MALATE, ZINC ION
Authors:Williams, M.K., Stec, B., Kantrowitz, E.R.
Deposit date:1998-04-25
Release date:1998-10-14
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.6 Å)
Cite:A single mutation in the regulatory chain of Escherichia coli aspartate transcarbamoylase results in an extreme T-state structure.
J.Mol.Biol., 281, 1998