Author results

1MDR
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THE ROLE OF LYSINE 166 IN THE MECHANISM OF MANDELATE RACEMASE FROM PSEUDOMONAS PUTIDA: MECHANISTIC AND CRYSTALLOGRAPHIC EVIDENCE FOR STEREOSPECIFIC ALKYLATION BY (R)-ALPHA-PHENYLGLYCIDATE
Descriptor:MANDELATE RACEMASE, MAGNESIUM ION, ATROLACTIC ACID (2-PHENYL-LACTIC ACID)
Authors:Landro, J.A., Gerlt, J.A., Kozarich, J.W., Koo, C.W., Shah, V.J., Kenyon, G.L., Neidhart, D.J., Fujita, S., Petsko, G.A.
Deposit date:1993-11-19
Release date:1994-08-31
Last modified:2017-11-29
Method:X-RAY DIFFRACTION (2.1 Å)
Cite:The role of lysine 166 in the mechanism of mandelate racemase from Pseudomonas putida: mechanistic and crystallographic evidence for stereospecific alkylation by (R)-alpha-phenylglycidate.
Biochemistry, 33, 1994
1EC7
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E. COLI GLUCARATE DEHYDRATASE NATIVE ENZYME
Descriptor:GLUCARATE DEHYDRATASE, MAGNESIUM ION, ISOPROPYL ALCOHOL
Authors:Gulick, A.M., Hubbard, B.K., Gerlt, J.A., Rayment, I.
Deposit date:2000-01-25
Release date:2000-05-23
Last modified:2018-02-28
Method:X-RAY DIFFRACTION (1.9 Å)
Cite:Evolution of enzymatic activities in the enolase superfamily: crystallographic and mutagenesis studies of the reaction catalyzed by D-glucarate dehydratase from Escherichia coli.
Biochemistry, 39, 2000
1EC8
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E. COLI GLUCARATE DEHYDRATASE BOUND TO PRODUCT 2,3-DIHYDROXY-5-OXO-HEXANEDIOATE
Descriptor:GLUCARATE DEHYDRATASE, MAGNESIUM ION, 2,3-DIHYDROXY-5-OXO-HEXANEDIOATE, ...
Authors:Gulick, A.M., Hubbard, B.K., Gerlt, J.A., Rayment, I.
Deposit date:2000-01-25
Release date:2000-05-23
Last modified:2018-02-28
Method:X-RAY DIFFRACTION (1.9 Å)
Cite:Evolution of enzymatic activities in the enolase superfamily: crystallographic and mutagenesis studies of the reaction catalyzed by D-glucarate dehydratase from Escherichia coli.
Biochemistry, 39, 2000
1EC9
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E. COLI GLUCARATE DEHYDRATASE BOUND TO XYLAROHYDROXAMATE
Descriptor:GLUCARATE DEHYDRATASE, MAGNESIUM ION, XYLAROHYDROXAMATE, ...
Authors:Gulick, A.M., Hubbard, B.K., Gerlt, J.A., Rayment, I.
Deposit date:2000-01-25
Release date:2000-05-23
Last modified:2018-02-28
Method:X-RAY DIFFRACTION (2 Å)
Cite:Evolution of enzymatic activities in the enolase superfamily: crystallographic and mutagenesis studies of the reaction catalyzed by D-glucarate dehydratase from Escherichia coli.
Biochemistry, 39, 2000
1ECQ
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E. COLI GLUCARATE DEHYDRATASE BOUND TO 4-DEOXYGLUCARATE
Descriptor:GLUCARATE DEHYDRATASE, 4-DEOXYGLUCARATE, MAGNESIUM ION, ...
Authors:Gulick, A.M., Hubbard, B.K., Gerlt, J.A., Rayment, I.
Deposit date:2000-01-25
Release date:2000-05-23
Last modified:2018-02-28
Method:X-RAY DIFFRACTION (2 Å)
Cite:Evolution of enzymatic activities in the enolase superfamily: crystallographic and mutagenesis studies of the reaction catalyzed by D-glucarate dehydratase from Escherichia coli.
Biochemistry, 39, 2000
1JCT
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GLUCARATE DEHYDRATASE, N341L MUTANT ORTHORHOMBIC FORM
Descriptor:Glucarate Dehydratase, MAGNESIUM ION, D-GLUCARATE, ...
Authors:Gulick, A.M., Hubbard, B.K., Gerlt, J.A., Rayment, I.
Deposit date:2001-06-11
Release date:2001-09-05
Last modified:2018-05-30
Method:X-RAY DIFFRACTION (2.75 Å)
Cite:Evolution of enzymatic activities in the enolase superfamily: identification of the general acid catalyst in the active site of D-glucarate dehydratase from Escherichia coli.
Biochemistry, 40, 2001
1JDF
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GLUCARATE DEHYDRATASE FROM E.COLI N341D MUTANT
Descriptor:Glucarate Dehydratase, MAGNESIUM ION, 2,3-DIHYDROXY-5-OXO-HEXANEDIOATE, ...
Authors:Gulick, A.M., Hubbard, B.K., Gerlt, J.A., Rayment, I.
Deposit date:2001-06-13
Release date:2001-09-05
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2 Å)
Cite:Evolution of enzymatic activities in the enolase superfamily: identification of the general acid catalyst in the active site of D-glucarate dehydratase from Escherichia coli.
Biochemistry, 40, 2001
1BFD
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BENZOYLFORMATE DECARBOXYLASE FROM PSEUDOMONAS PUTIDA
Descriptor:BENZOYLFORMATE DECARBOXYLASE, CALCIUM ION, MAGNESIUM ION, ...
Authors:Hasson, M.S., Muscate, A., Mcleish, M.J., Polovnikova, L.S., Gerlt, J.A., Kenyon, G.L., Petsko, G.A., Ringe, D.
Deposit date:1998-04-30
Release date:1998-06-24
Last modified:2011-11-16
Method:X-RAY DIFFRACTION (1.6 Å)
Cite:The crystal structure of benzoylformate decarboxylase at 1.6 A resolution: diversity of catalytic residues in thiamin diphosphate-dependent enzymes.
Biochemistry, 37, 1998
1BKH
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MUCONATE LACTONIZING ENZYME FROM PSEUDOMONAS PUTIDA
Descriptor:MUCONATE LACTONIZING ENZYME
Authors:Hasson, M.S., Schlichting, I., Moulai, J., Taylor, K., Barrett, W., Kenyon, G.L., Babbitt, P.C., Gerlt, J.A., Petsko, G.A., Ringe, D.
Deposit date:1998-07-07
Release date:1998-10-21
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.1 Å)
Cite:Evolution of an enzyme active site: the structure of a new crystal form of muconate lactonizing enzyme compared with mandelate racemase and enolase.
Proc.Natl.Acad.Sci.USA, 95, 1998
1BQG
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THE STRUCTURE OF THE D-GLUCARATE DEHYDRATASE PROTEIN FROM PSEUDOMONAS PUTIDA
Descriptor:D-GLUCARATE DEHYDRATASE
Authors:Gulick, A.M., Palmer, D.R.J., Babbitt, P.C., Gerlt, J.A., Rayment, I.
Deposit date:1998-08-15
Release date:1999-05-18
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.3 Å)
Cite:Evolution of enzymatic activities in the enolase superfamily: crystal structure of (D)-glucarate dehydratase from Pseudomonas putida.
Biochemistry, 37, 1998
1EF8
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CRYSTAL STRUCTURE OF METHYLMALONYL COA DECARBOXYLASE
Descriptor:METHYLMALONYL COA DECARBOXYLASE, NICKEL (II) ION
Authors:Benning, M.M., Haller, T., Gerlt, J.A., Holden, H.M.
Deposit date:2000-02-07
Release date:2000-05-24
Last modified:2017-10-04
Method:X-RAY DIFFRACTION (1.85 Å)
Cite:New reactions in the crotonase superfamily: structure of methylmalonyl CoA decarboxylase from Escherichia coli.
Biochemistry, 39, 2000
1EF9
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THE CRYSTAL STRUCTURE OF METHYLMALONYL COA DECARBOXYLASE COMPLEXED WITH 2S-CARBOXYPROPYL COA
Descriptor:METHYLMALONYL COA DECARBOXYLASE, 2-CARBOXYPROPYL-COENZYME A
Authors:Benning, M.M., Haller, T., Gerlt, J.A., Holden, H.M.
Deposit date:2000-02-07
Release date:2000-05-24
Last modified:2017-10-04
Method:X-RAY DIFFRACTION (2.7 Å)
Cite:New reactions in the crotonase superfamily: structure of methylmalonyl CoA decarboxylase from Escherichia coli.
Biochemistry, 39, 2000
1FHU
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CRYSTAL STRUCTURE ANALYSIS OF O-SUCCINYLBENZOATE SYNTHASE FROM E. COLI
Descriptor:O-SUCCINYLBENZOATE SYNTHASE
Authors:Rayment, I., Thompson, T.B., Gerlt, J.A.
Deposit date:2000-08-02
Release date:2000-08-23
Last modified:2018-01-31
Method:X-RAY DIFFRACTION (1.65 Å)
Cite:Evolution of enzymatic activity in the enolase superfamily: structure of o-succinylbenzoate synthase from Escherichia coli in complex with Mg2+ and o-succinylbenzoate.
Biochemistry, 39, 2000
1FHV
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CRYSTAL STRUCTURE ANALYSIS OF O-SUCCINYLBENZOATE SYNTHASE FROM E. COLI COMPLEXED WITH MG AND OSB
Descriptor:O-SUCCINYLBENZOATE SYNTHASE, MAGNESIUM ION, 2-SUCCINYLBENZOATE
Authors:Rayment, I., Thompson, T.B., Gerlt, J.A.
Deposit date:2000-08-02
Release date:2000-08-23
Last modified:2018-01-31
Method:X-RAY DIFFRACTION (1.77 Å)
Cite:Evolution of enzymatic activity in the enolase superfamily: structure of o-succinylbenzoate synthase from Escherichia coli in complex with Mg2+ and o-succinylbenzoate.
Biochemistry, 39, 2000
1JPD
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L-ALA-D/L-GLU EPIMERASE
Descriptor:L-Ala-D/L-Glu Epimerase
Authors:Gulick, A.M., Schmidt, D.M.Z., Gerlt, J.A., Rayment, I.
Deposit date:2001-08-01
Release date:2001-12-21
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.6 Å)
Cite:Evolution of enzymatic activities in the enolase superfamily: crystal structures of the L-Ala-D/L-Glu epimerases from Escherichia coli and Bacillus subtilis.
Biochemistry, 40, 2001
1JPM
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L-ALA-D/L-GLU EPIMERASE
Descriptor:L-Ala-D/L-Glu Epimerase, MAGNESIUM ION, GLYCEROL
Authors:Gulick, A.M., Schmidt, D.M.Z., Gerlt, J.A., Rayment, I.
Deposit date:2001-08-02
Release date:2001-12-21
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.25 Å)
Cite:Evolution of enzymatic activities in the enolase superfamily: crystal structures of the L-Ala-D/L-Glu epimerases from Escherichia coli and Bacillus subtilis.
Biochemistry, 40, 2001
1KV8
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CRYSTAL STRUCTURE OF 3-KETO-L-GULONATE 6-PHOSPHATE DECARBOXYLASE
Descriptor:3-Keto-L-Gulonate 6-Phosphate Decarboxylase, PHOSPHATE ION, MAGNESIUM ION
Authors:Wise, E., Yew, W.S., Babbitt, P.C., Gerlt, J.A., Rayment, I.
Deposit date:2002-01-25
Release date:2002-04-15
Last modified:2011-11-16
Method:X-RAY DIFFRACTION (1.62 Å)
Cite:Homologous (beta/alpha)8-barrel enzymes that catalyze unrelated reactions: orotidine 5'-monophosphate decarboxylase and 3-keto-L-gulonate 6-phosphate decarboxylase.
Biochemistry, 41, 2002
1KW1
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CRYSTAL STRUCTURE OF 3-KETO-L-GULONATE 6-PHOSPHATE DECARBOXYLASE WITH BOUND L-GULONATE 6-PHOSPHATE
Descriptor:3-Keto-L-Gulonate 6-Phosphate Decarboxylase, MAGNESIUM ION, L-GULURONIC ACID 6-PHOSPHATE
Authors:Wise, E., Yew, W.S., Babbitt, P.C., Gerlt, J.A., Rayment, I.
Deposit date:2002-01-28
Release date:2002-04-15
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.2 Å)
Cite:Homologous (beta/alpha)8-barrel enzymes that catalyze unrelated reactions: orotidine 5'-monophosphate decarboxylase and 3-keto-L-gulonate 6-phosphate decarboxylase.
Biochemistry, 41, 2002
1Q6L
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STRUCTURE OF 3-KETO-L-GULONATE 6-PHOSPHATE DECARBOXYLASE WITH BOUND L-THREONOHYDROXAMATE 4-PHOSPHATE
Descriptor:3-keto-L-gulonate 6-phosphate decarboxylase, MAGNESIUM ION, L-THREONOHYDROXAMATE 4-PHOSPHATE
Authors:Wise, E.L., Yew, W.S., Gerlt, J.A., Rayment, I.
Deposit date:2003-08-13
Release date:2003-10-28
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.8 Å)
Cite:Structural Evidence for a 1,2-Enediolate Intermediate in the Reaction Catalyzed by 3-Keto-l-Gulonate 6-Phosphate Decarboxylase, a Member of the Orotidine 5'-Monophosphate Decarboxylase Suprafamily
Biochemistry, 42, 2003
1Q6O
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STRUCTURE OF 3-KETO-L-GULONATE 6-PHOSPHATE DECARBOXYLASE WITH BOUND L-GULONAET 6-PHOSPHATE
Descriptor:3-keto-L-gulonate 6-phosphate decarboxylase, MAGNESIUM ION, L-GULURONIC ACID 6-PHOSPHATE
Authors:Wise, E.L., Yew, W.S., Gerlt, J.A., Rayment, I.
Deposit date:2003-08-13
Release date:2003-10-28
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.202 Å)
Cite:Structural Evidence for a 1,2-Enediolate Intermediate in the Reaction Catalyzed by 3-Keto-l-Gulonate 6-Phosphate Decarboxylase, a Member of the Orotidine 5'-Monophosphate Decarboxylase Suprafamily
Biochemistry, 42, 2003
1Q6Q
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STRUCTURE OF 3-KETO-L-GULONATE 6-PHOSPHATE DECARBOXYLASE WITH BOUND XYLITOL 5-PHOSPHATE
Descriptor:3-keto-L-gulonate 6-phosphate decarboxylase, MAGNESIUM ION, L-XYLITOL 5-PHOSPHATE
Authors:Wise, E.L., Yew, W.S., Gerlt, J.A., Rayment, I.
Deposit date:2003-08-13
Release date:2003-10-28
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.695 Å)
Cite:Structural Evidence for a 1,2-Enediolate Intermediate in the Reaction Catalyzed by 3-Keto-l-Gulonate 6-Phosphate Decarboxylase, a Member of the Orotidine 5'-Monophosphate Decarboxylase Suprafamily
Biochemistry, 42, 2003
1Q6R
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STRUCTURE OF 3-KETO-L-GULONATE 6-PHOSPHATE DECARBOXYLASE WITH BOUND L-XYLULOSE 5-PHOSPHATE
Descriptor:3-keto-L-gulonate 6-phosphate decarboxylase, MAGNESIUM ION, L-XYLULOSE 5-PHOSPHATE
Authors:Wise, E.L., Yew, W.S., Gerlt, J.A., Rayment, I.
Deposit date:2003-08-13
Release date:2003-10-28
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.76 Å)
Cite:Structural Evidence for a 1,2-Enediolate Intermediate in the Reaction Catalyzed by 3-Keto-l-Gulonate 6-Phosphate Decarboxylase, a Member of the Orotidine 5'-Monophosphate Decarboxylase Suprafamily
Biochemistry, 42, 2003
1R6W
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CRYSTAL STRUCTURE OF THE K133R MUTANT OF O-SUCCINYLBENZOATE SYNTHASE (OSBS) FROM ESCHERICHIA COLI. COMPLEX WITH SHCHC
Descriptor:o-Succinylbenzoate Synthase, MAGNESIUM ION, 2-(3-CARBOXYPROPIONYL)-6-HYDROXY-CYCLOHEXA-2,4-DIENE CARBOXYLIC ACID
Authors:Klenchin, V.A., Taylor Ringia, E.A., Gerlt, J.A., Rayment, I.
Deposit date:2003-10-17
Release date:2003-11-25
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.62 Å)
Cite:Evolution of Enzymatic Activity in the Enolase Superfamily: Structural and Mutagenic Studies of the Mechanism of the Reaction Catalyzed by o-Succinylbenzoate Synthase from Escherichia coli
Biochemistry, 42, 2003
1RVK
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CRYSTAL STRUCTURE OF ENOLASE AGR_L_2751 FROM AGROBACTERIUM TUMEFACIENS
Descriptor:isomerase/lactonizing enzyme, MAGNESIUM ION
Authors:Fedorov, A.A., Fedorov, E.V., Thirumuruhan, R., Zencheck, W., Millikin, C., Gerlt, J.A., Almo, S.C., Burley, S.K., New York SGX Research Center for Structural Genomics (NYSGXRC)
Deposit date:2003-12-14
Release date:2003-12-23
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.7 Å)
Cite:Evolution of enzymatic activites in the Enolase superfamily: 1.7 A crystal structure of the hypothetical protein MR.GI-17937161 from Agrobacterium tumefaciens
To be Published
1SJA
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X-RAY STRUCTURE OF O-SUCCINYLBENZOATE SYNTHASE COMPLEXED WITH N-ACETYLMETHIONINE
Descriptor:N-acylamino acid racemase, MAGNESIUM ION, N-ACETYLMETHIONINE
Authors:Thoden, J.B., Taylor-Ringia, E.A., Garrett, J.B., Gerlt, J.A., Holden, H.M., Rayment, I.
Deposit date:2004-03-03
Release date:2004-06-01
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.3 Å)
Cite:Evolution of Enzymatic Activity in the Enolase Superfamily: Structural Studies of the Promiscuous o-Succinylbenzoate Synthase from Amycolatopsis
Biochemistry, 43, 2004