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4B43
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BACTERIAL TRANSLATION INITIATION FACTOR IF2 (1-363), APO FORM, DOUBLE MUTANT K86L H130A
Descriptor:TRANSLATION INITIATION FACTOR IF-2, MAGNESIUM ION, GLYCEROL, ...
Authors:Simonetti, A., Urzhumtsev, A., Klaholz, B.P.
Deposit date:2012-07-27
Release date:2013-09-18
Method:X-RAY DIFFRACTION (1.937 Å)
Cite:Molecular Basis of GTP Hydrolysis by Bacterial Translation Initiation Factor If2
To be Published
4B3X
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BACTERIAL TRANSLATION INITIATION FACTOR IF2 (1-363), APO FORM
Descriptor:TRANSLATION INITIATION FACTOR IF-2, GLYCEROL
Authors:Simonetti, A., Urzhumtsev, A., Klaholz, B.P.
Deposit date:2012-07-26
Release date:2013-05-29
Last modified:2013-06-05
Method:X-RAY DIFFRACTION (1.95 Å)
Cite:Structure of the Protein Core of Translation Initiation Factor 2 in Apo, GTP-Bound and Gdp-Bound Forms
Acta Crystallogr.,Sect.D, 69, 2013
4B47
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BACTERIAL TRANSLATION INITIATION FACTOR IF2 (1-363), COMPLEX WITH GDP AT PH6.5
Descriptor:TRANSLATION INITIATION FACTOR IF-2, GUANOSINE-5'-DIPHOSPHATE, MAGNESIUM ION
Authors:Simonetti, A., Urzhumtsev, A., Klaholz, B.P.
Deposit date:2012-07-27
Release date:2013-05-29
Last modified:2015-02-11
Method:X-RAY DIFFRACTION (2.301 Å)
Cite:Structure of the Protein Core of Translation Initiation Factor 2 in Apo, GTP-Bound and Gdp-Bound Forms
Acta Crystallogr.,Sect.D, 69, 2013
4B44
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BACTERIAL TRANSLATION INITIATION FACTOR IF2 (1-363), COMPLEX WITH GDP AT PH8.0
Descriptor:TRANSLATION INITIATION FACTOR IF-2, GUANOSINE-5'-DIPHOSPHATE
Authors:Simonetti, A., Urzhumtsev, A., Klaholz, B.P.
Deposit date:2012-07-27
Release date:2013-09-18
Last modified:2015-02-11
Method:X-RAY DIFFRACTION (2.7 Å)
Cite:Molecular Basis of GTP Hydrolysis by Bacterial Translation Initiation Factor If2
To be Published
4B48
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BACTERIAL TRANSLATION INITIATION FACTOR IF2 (1-363), COMPLEX WITH GTP
Descriptor:TRANSLATION INITIATION FACTOR IF-2, GUANOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION
Authors:Simonetti, A., Urzhumtsev, A., Klaholz, B.P.
Deposit date:2012-07-27
Release date:2013-05-29
Last modified:2013-06-05
Method:X-RAY DIFFRACTION (2.8 Å)
Cite:Structure of the Protein Core of Translation Initiation Factor 2 in Apo, GTP-Bound and Gdp-Bound Forms
Acta Crystallogr.,Sect.D, 69, 2013
5DM6
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CRYSTAL STRUCTURE OF THE 50S RIBOSOMAL SUBUNIT FROM DEINOCOCCUS RADIODURANS
Descriptor:50S ribosomal protein L1, 50S ribosomal protein L2, 50S ribosomal protein L3, ...
Authors:Kaminishi, T., Schedlbauer, A., Ochoa-Lizarralde, B., Connell, S.R., Fucini, P.
Deposit date:2015-09-08
Release date:2015-11-11
Last modified:2016-02-03
Method:X-RAY DIFFRACTION (2.9 Å)
Cite:Crystallographic characterization of the ribosomal binding site and molecular mechanism of action of Hygromycin A.
Nucleic Acids Res., 43, 2015
4V9R
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CRYSTAL STRUCTURE OF ANTIBIOTIC DITYROMYCIN BOUND TO 70S RIBOSOME
Descriptor:16S Ribosomal RNA, 30S Ribosomal Protein S2, 30S Ribosomal Protein S3, ...
Authors:Bulkley, D.P., Brandi, L., Polikanov, Y.S., Fabbretti, A., O'Connor, M., Gualerzi, C.O., Steitz, T.A.
Deposit date:2013-12-05
Release date:2014-07-09
Last modified:2014-12-10
Method:X-RAY DIFFRACTION (3 Å)
Cite:The antibiotics dityromycin and GE82832 bind protein S12 and block EF-G-catalyzed translocation.
Cell Rep, 6, 2014
5DM7
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CRYSTAL STRUCTURE OF THE 50S RIBOSOMAL SUBUNIT FROM DEINOCOCCUS RADIODURANS IN COMPLEX WITH HYGROMYCIN A
Descriptor:50S ribosomal protein L1, 50S ribosomal protein L2, 50S ribosomal protein L3, ...
Authors:Kaminishi, T., Schedlbauer, A., Ochoa-Lizarralde, B., Connell, S.R., Fucini, P.
Deposit date:2015-09-08
Release date:2015-11-11
Last modified:2015-11-25
Method:X-RAY DIFFRACTION (3 Å)
Cite:Crystallographic characterization of the ribosomal binding site and molecular mechanism of action of Hygromycin A.
Nucleic Acids Res., 43, 2015
4V9S
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CRYSTAL STRUCTURE OF ANTIBIOTIC GE82832 BOUND TO 70S RIBOSOME
Descriptor:16S Ribosomal RNA, 30S Ribosomal Protein S2, 30S Ribosomal Protein S3, ...
Authors:Bulkley, D.P., Brandi, L., Polikanov, Y.S., Fabbretti, A., O'Connor, M., Gualerzi, C.O., Steitz, T.A.
Deposit date:2013-12-05
Release date:2014-07-09
Last modified:2018-06-27
Method:X-RAY DIFFRACTION (3.1 Å)
Cite:The antibiotics dityromycin and GE82832 bind protein S12 and block EF-G-catalyzed translocation.
Cell Rep, 6, 2014
5IWA
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CRYSTAL STRUCTURE OF THE 30S RIBOSOMAL SUBUNIT FROM THERMUS THERMOPHILUS IN COMPLEX WITH THE GE81112 PEPTIDE ANTIBIOTIC
Descriptor:30S ribosomal protein S2, 30S ribosomal protein S3, 30S ribosomal protein S4, ...
Authors:Schedlbauer, A., Kaminishi, T., Ochoa-Lizarralde, B., Chieko, N., Masahito, K., Takemoto, C., Yokoyama, S., Connell, S.R., Fucini, P.
Deposit date:2016-03-22
Release date:2016-04-27
Last modified:2017-04-05
Method:X-RAY DIFFRACTION (3.5 Å)
Cite:Inhibition of translation initiation complex formation by GE81112 unravels a 16S rRNA structural switch involved in P-site decoding.
Proc.Natl.Acad.Sci.USA, 113, 2016
3J4J
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MODEL OF FULL-LENGTH T. THERMOPHILUS TRANSLATION INITIATION FACTOR 2 REFINED AGAINST ITS CRYO-EM DENSITY FROM A 30S INITIATION COMPLEX MAP
Descriptor:Translation initiation factor IF-2
Authors:Simonetti, A., Marzi, S., Billas, I.M.L., Tsai, A., Fabbretti, A., Myasnikov, A., Roblin, P., Vaiana, A.C., Hazemann, I., Eiler, D., Steitz, T.A., Puglisi, J.D., Gualerzi, C.O., Klaholz, B.P.
Deposit date:2013-08-26
Release date:2013-09-25
Last modified:2018-07-18
Method:ELECTRON MICROSCOPY (11.5 Å)
Cite:Involvement of protein IF2 N domain in ribosomal subunit joining revealed from architecture and function of the full-length initiation factor.
Proc.Natl.Acad.Sci.USA, 110, 2013
5ME0
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STRUCTURE OF THE 30S PRE-INITIATION COMPLEX 1 (30S IC-1) STALLED BY GE81112
Descriptor:16S ribosomal RNA, 30S ribosomal protein S2, 30S ribosomal protein S3, ...
Authors:Lopez-Alonso, J.P., Fabbretti, A., Kaminishi, T., Iturrioz, I., Brandi, L., Gil Carton, D., Gualerzi, C., Fucini, P., Connell, S.
Deposit date:2016-11-14
Release date:2017-01-11
Last modified:2018-11-21
Method:ELECTRON MICROSCOPY (13.5 Å)
Cite:Structure of a 30S pre-initiation complex stalled by GE81112 reveals structural parallels in bacterial and eukaryotic protein synthesis initiation pathways.
Nucleic Acids Res., 45, 2017
5ME1
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STRUCTURE OF THE 30S PRE-INITIATION COMPLEX 2 (30S IC-2) STALLED BY GE81112
Descriptor:16S ribosomal RNA, 30S ribosomal protein S2, 30S ribosomal protein S3, ...
Authors:Lopez-Alonso, J.P., Fabbretti, A., Kaminishi, T., Iturrioz, I., Brandi, L., Gil Carton, D., Gualerzi, C., Fucini, P., Connell, S.
Deposit date:2016-11-14
Release date:2017-01-11
Last modified:2018-11-21
Method:ELECTRON MICROSCOPY (13.5 Å)
Cite:Structure of a 30S pre-initiation complex stalled by GE81112 reveals structural parallels in bacterial and eukaryotic protein synthesis initiation pathways.
Nucleic Acids Res., 45, 2017