Author results

2XE0
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MOLECULAR BASIS OF ENGINEERED MEGANUCLEASE TARGETING OF THE ENDOGENOUS HUMAN RAG1 LOCUS
Descriptor:I-CREI V2V3 VARIANT, 24MER DNA, ACETATE ION, ...
Authors:Munoz, I.G., Prieto, J., Subramanian, S., Coloma, J., Redondo, P., Villate, M., Merino, N., Marenchino, M., D'Abramo, M., Gervasio, F.L., Grizot, S., Daboussi, F., Smith, J., Chion-Sotine, I., Paques, F., Duchateau, P., Alibes, A., Stricher, F., Serrano, L., Blanco, F.J., Montoya, G.
Deposit date:2010-05-10
Release date:2010-09-29
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.31 Å)
Cite:Molecular Basis of Engineered Meganuclease Targeting of the Endogenous Human Rag1 Locus
Nucleic Acids Res., 39, 2011
4AAB
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CRYSTAL STRUCTURE OF THE MUTANT D75N I-CREI IN COMPLEX WITH ITS WILD- TYPE TARGET (THE FOUR CENTRAL BASES, 2NN REGION, ARE COMPOSED BY GTAC FROM 5' TO 3')
Descriptor:DNA ENDONUCLEASE I-CREI, 14MER DNA 5'-D(*TP*CP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*AP*CP)-3', 10MER DNA 5'-D(*GP*AP*CP*GP*TP*TP*TP*TP*GP*AP)-3', ...
Authors:Molina, R., Redondo, P., Stella, S., Marenchino, M., D'Abramo, M., Gervasio, F.L., Epinat, J.C., Valton, J., Grizot, S., Duchateau, P., Prieto, J., Montoya, G.
Deposit date:2011-12-01
Release date:2012-05-02
Last modified:2012-08-22
Method:X-RAY DIFFRACTION (2.5 Å)
Cite:Non-Specific Protein-DNA Interactions Control I-Crei Target Binding and Cleavage.
Nucleic Acids Res., 40, 2012
4AAD
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CRYSTAL STRUCTURE OF THE MUTANT D75N I-CREI IN COMPLEX WITH ITS WILD- TYPE TARGET IN ABSENCE OF METAL IONS AT THE ACTIVE SITE (THE FOUR CENTRAL BASES, 2NN REGION, ARE COMPOSED BY GTAC FROM 5' TO 3')
Descriptor:DNA ENDONUCLEASE I-CREI, 24MER DNA, GLYCEROL
Authors:Molina, R., Redondo, P., Stella, S., Marenchino, M., D'Abramo, M., Gervasio, F.L., Epinat, J.C., Valton, J., Grizot, S., Duchateau, P., Prieto, J., Montoya, G.
Deposit date:2011-12-01
Release date:2012-05-02
Last modified:2012-08-22
Method:X-RAY DIFFRACTION (3.1 Å)
Cite:Non-Specific Protein-DNA Interactions Control I-Crei Target Binding and Cleavage.
Nucleic Acids Res., 40, 2012
4AAE
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CRYSTAL STRUCTURE OF THE MUTANT D75N I-CREI IN COMPLEX WITH AN ALTERED TARGET (THE FOUR CENTRAL BASES, 2NN REGION, ARE COMPOSED BY AGCG FROM 5' TO 3')
Descriptor:DNA ENDONUCLEASE I-CREI, 24MER DNA
Authors:Molina, R., Redondo, P., Stella, S., Marenchino, M., D'Abramo, M., Gervasio, F.L., Epinat, J.C., Valton, J., Grizot, S., Duchateau, P., Prieto, J., Montoya, G.
Deposit date:2011-12-01
Release date:2012-05-02
Last modified:2012-08-22
Method:X-RAY DIFFRACTION (2.6 Å)
Cite:Non-Specific Protein-DNA Interactions Control I-Crei Target Binding and Cleavage.
Nucleic Acids Res., 40, 2012
4AAF
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CRYSTAL STRUCTURE OF THE MUTANT D75N I-CREI IN COMPLEX WITH AN ALTERED TARGET (THE FOUR CENTRAL BASES, 2NN REGION, ARE COMPOSED BY TGCA FROM 5' TO 3')
Descriptor:DNA ENDONUCLEASE I-CREI, 24MER DNA, 1,2-ETHANEDIOL
Authors:Molina, R., Redondo, P., Stella, S., Marenchino, M., D'Abramo, M., Gervasio, F.L., Epinat, J.C., Valton, J., Grizot, S., Duchateau, P., Prieto, J., Montoya, G.
Deposit date:2011-12-01
Release date:2012-05-02
Last modified:2012-08-22
Method:X-RAY DIFFRACTION (2.5 Å)
Cite:Non-Specific Protein-DNA Interactions Control I-Crei Target Binding and Cleavage.
Nucleic Acids Res., 40, 2012
4AAG
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CRYSTAL STRUCTURE OF THE MUTANT D75N I-CREI IN COMPLEX WITH ITS WILD- TYPE TARGET IN PRESENCE OF CA AT THE ACTIVE SITE (THE FOUR CENTRAL BASES, 2NN REGION, ARE COMPOSED BY GTAC FROM 5' TO 3')
Descriptor:DNA ENDONUCLEASE I-CREI, 5'-D(*TP*CP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*AP*CP *GP*AP*CP*GP*TP*TP*TP*TP*GP*A)-3', CALCIUM ION
Authors:Molina, R., Redondo, P., Stella, S., Marenchino, M., D'Abramo, M., Gervasio, F., Epinat, J.C., Valton, J., Grizot, S., Duchateau, P., Prieto, J., Montoya, G.
Deposit date:2011-12-01
Release date:2012-05-02
Last modified:2012-08-22
Method:X-RAY DIFFRACTION (2.8 Å)
Cite:Non-Specific Protein-DNA Interactions Control I-Crei Target Binding and Cleavage.
Nucleic Acids Res., 40, 2012
5AK9
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THE CRYSTAL STRUCTURE OF I-DMOI Q42AK120M IN COMPLEX WITH ITS TARGET DNA IN THE PRESENCE OF 2MM MN
Descriptor:HOMING ENDONUCLEASE I-DMOI, 5'-D(*GP*CP*CP*TP*TP*GP*CP*CP*GP*GP*GP*TP*AP*AP)-3', 5'-D(*GP*TP*TP*CP*CP*GP*GP*CP*GP*CP*GP)-3, ...
Authors:Molina, R., Marcaida, M.J., Redondo, P., Marenchino, M., D'Abramo, M., Montoya, G., Prieto, J.
Deposit date:2015-03-02
Release date:2015-06-17
Last modified:2017-08-23
Method:X-RAY DIFFRACTION (2.601 Å)
Cite:Engineering a Nickase on the Homing Endonuclease I-Dmoi Scaffold.
J.Biol.Chem., 290, 2015
5AKF
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THE CRYSTAL STRUCTURE OF I-DMOI Q42AK120M IN COMPLEX WITH ITS TARGET DNA NICKED IN THE CODING STRAND A AND IN THE PRESENCE OF 2MM MN
Descriptor:HOMING ENDONUCLEASE I-DMOI, 5'-D(*GP*CP*CP*TP*TP*GP*CP*CP*GP*GP*GP*TP*AP*AP)-3', 5'-D(*GP*TP*TP*CP*CP*GP*GP*CP*GP*CP*GP)-3', ...
Authors:Molina, R., Marcaida, M.J., Redondo, P., Marenchino, M., D'Abramo, M., Montoya, G., Prieto, J.
Deposit date:2015-03-03
Release date:2015-06-17
Last modified:2017-08-23
Method:X-RAY DIFFRACTION (2.45 Å)
Cite:Engineering a Nickase on the Homing Endonuclease I-Dmoi Scaffold.
J.Biol.Chem., 290, 2015
5AKM
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THE CRYSTAL STRUCTURE OF I-DMOI G20S IN COMPLEX WITH ITS TARGET DNA IN THE PRESENCE OF 2MM MG
Descriptor:HOMING ENDONUCLEASE I-DMOI, 5'-D(*GP*CP*CP*TP*TP*GP*CP*CP*GP*GP*GP*TP*AP*AP)-3', 5'-D(*GP*TP*TP*CP*CP*GP*GP*CP*GP*CP*GP)-3', ...
Authors:Molina, R., Marcaida, M.J., Redondo, P., Marenchino, M., D'Abramo, M., Montoya, G., Prieto, J.
Deposit date:2015-03-04
Release date:2015-06-17
Last modified:2017-08-23
Method:X-RAY DIFFRACTION (2.4 Å)
Cite:Engineering a Nickase on the Homing Endonuclease I-Dmoi Scaffold.
J.Biol.Chem., 290, 2015
5AKN
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THE CRYSTAL STRUCTURE OF I-DMOI Q42AK120M IN COMPLEX WITH ITS TARGET DNA NICKED IN THE NON-CODING STRAND B AND IN THE PRESENCE OF 2MM MN
Descriptor:HOMING ENDONUCLEASE I-DMOI, 5'-D(*GP*CP*CP*TP*TP*GP*CP*CP*GP*GP*GP*TP*AP*AP)-3', 5'-D(*GP*TP*TP*CP*CP*GP*GP*CP*GP*CP*GP)-3, ...
Authors:Molina, R., Marcaida, M.J., Redondo, P., Marenchino, M., D'Abramo, M., Montoya, G., Prieto, J.
Deposit date:2015-03-04
Release date:2015-06-17
Last modified:2015-08-05
Method:X-RAY DIFFRACTION (2.75 Å)
Cite:Engineering a Nickase on the Homing Endonuclease I-Dmoi Scaffold.
J.Biol.Chem., 290, 2015
5A0W
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THE CRYSTAL STRUCTURE OF I-DMOI E117A IN COMPLEX WITH ITS TARGET DNA AND IN THE PRESENCE OF 2MM MN
Descriptor:HOMING ENDONUCLEASE I-DMOI, 25MER, CHLORIDE ION, ...
Authors:Molina, R., Besker, N., Prieto, J., Montoya, G., D'Abramo, M.
Deposit date:2015-04-23
Release date:2016-05-11
Method:X-RAY DIFFRACTION (2.2 Å)
Cite:Structural and Dynamical Characterization of the I- Dmo Catalytic Activity
To be Published
3MX9
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MOLECULAR BASIS OF ENGINEERED MEGANUCLEASE TARGETING OF THE ENDOGENOUS HUMAN RAG1 LOCUS
Descriptor:Protein scV3V2(G19S), DNA (5'-D(*TP*TP*GP*TP*TP*CP*TP*CP*AP*GP*GP*TP*AP*CP*CP*TP*CP*AP*GP*CP*CP*AP*GP*A)-3'), DNA (5'-D(*TP*CP*TP*GP*GP*CP*TP*GP*AP*GP*GP*TP*AP*CP*CP*TP*GP*AP*GP*AP*AP*CP*AP*A)-3'), ...
Authors:Munoz, I.G., Prieto, J., Subramanian, S., Coloma, J., Montoya, G.
Deposit date:2010-05-07
Release date:2010-10-06
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.6 Å)
Cite:Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus.
Nucleic Acids Res., 39, 2011
3MXA
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MOLECULAR BASIS OF ENGINEERED MEGANUCLEASE TARGETING OF THE ENDOGENOUS HUMAN RAG1 LOCUS
Descriptor:scV3V2(G19S), DNA (5'-D(*TP*TP*GP*TP*TP*CP*TP*CP*AP*GP*GP*TP*AP*C)-3'), DNA (5'-D(P*CP*TP*CP*AP*GP*CP*CP*AP*GP*A)-3'), ...
Authors:Munoz, I.G., Prieto, J., Subramanian, S., Coloma, J., Montoya, G.
Deposit date:2010-05-07
Release date:2010-10-06
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.3 Å)
Cite:Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus.
Nucleic Acids Res., 39, 2011
3MXB
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MOLECULAR BASIS OF ENGINEERED MEGANUCLEASE TARGETING OF THE ENDOGENOUS HUMAN RAG1 LOCUS
Descriptor:V3(E8K), V2(K7E-G19S), DNA (5'-D(*TP*TP*GP*TP*TP*CP*TP*CP*AP*GP*GP*TP*AP*CP*CP*TP*CP*AP*GP*CP*CP*AP*GP*A)-3'), ...
Authors:Munoz, I.G., Prieto, J., Subramanian, S., Coloma, J., Montoya, G.
Deposit date:2010-05-07
Release date:2010-10-06
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.3 Å)
Cite:Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus.
Nucleic Acids Res., 39, 2011
6HFD
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HUMAN DIHYDROOROTASE MUTANT F1563L APO STRUCTURE
Descriptor:CAD protein, ZINC ION, FORMIC ACID
Authors:Ramon-Maiques, S., Grande Garcia, A.
Deposit date:2018-08-21
Release date:2018-10-24
Last modified:2018-12-19
Method:X-RAY DIFFRACTION (1.869968 Å)
Cite:Characterization of the catalytic flexible loop in the dihydroorotase domain of the human multi-enzymatic protein CAD.
J. Biol. Chem., 293, 2018
6HFE
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HUMAN DIHYDROOROTASE MUTANT F1563T APO STRUCTURE
Descriptor:CAD protein, ZINC ION, FORMIC ACID
Authors:Ramon-Maiques, S., Grande Garcia, A.
Deposit date:2018-08-21
Release date:2018-10-24
Last modified:2018-12-19
Method:X-RAY DIFFRACTION (1.479974 Å)
Cite:Characterization of the catalytic flexible loop in the dihydroorotase domain of the human multi-enzymatic protein CAD.
J. Biol. Chem., 293, 2018
6HFF
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HUMAN DIHYDROOROTASE MUTANT F1563Y APO STRUCTURE
Descriptor:CAD protein, ZINC ION, FORMIC ACID
Authors:Ramon-Maiques, S., Grande Garcia, A.
Deposit date:2018-08-21
Release date:2018-10-24
Last modified:2018-12-19
Method:X-RAY DIFFRACTION (1.510001 Å)
Cite:Characterization of the catalytic flexible loop in the dihydroorotase domain of the human multi-enzymatic protein CAD.
J. Biol. Chem., 293, 2018
6HFH
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HUMAN DIHYDROOROTASE MUTANT F1563A CO-CRYSTALLIZED WITH CARBAMOYL ASPARTATE AT PH 7.0
Descriptor:CAD protein, ZINC ION, FORMIC ACID, ...
Authors:Ramon-Maiques, S., Grande Garcia, A.
Deposit date:2018-08-21
Release date:2018-10-24
Last modified:2018-12-19
Method:X-RAY DIFFRACTION (1.448713 Å)
Cite:Characterization of the catalytic flexible loop in the dihydroorotase domain of the human multi-enzymatic protein CAD.
J. Biol. Chem., 293, 2018
6HFI
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HUMAN DIHYDROOROTASE MUTANT F1563A APO STRUCTURE
Descriptor:CAD protein, ZINC ION, FORMIC ACID
Authors:Ramon-Maiques, S., Grande Garcia, A.
Deposit date:2018-08-21
Release date:2018-10-24
Last modified:2018-12-19
Method:X-RAY DIFFRACTION (1.459947 Å)
Cite:Characterization of the catalytic flexible loop in the dihydroorotase domain of the human multi-enzymatic protein CAD.
J. Biol. Chem., 293, 2018
6HFJ
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HUMAN DIHYDROOROTASE MUTANT F1563A CO-CRYSTALLIZED WITH CARBAMOYL ASPARTATE AT PH 7.5
Descriptor:CAD protein, ZINC ION, FORMIC ACID, ...
Authors:Ramon-Maiques, S., Grande Garcia, A.
Deposit date:2018-08-21
Release date:2018-10-24
Last modified:2018-12-19
Method:X-RAY DIFFRACTION (1.2 Å)
Cite:Characterization of the catalytic flexible loop in the dihydroorotase domain of the human multi-enzymatic protein CAD.
J. Biol. Chem., 293, 2018
6HFK
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HUMAN DIHYDROOROTASE MUTANT F1563L CO-CRYSTALLIZED WITH CARBAMOYL ASPARTATE AT PH 6.5
Descriptor:CAD protein, ZINC ION, FORMIC ACID, ...
Authors:Ramon-Maiques, S., Grande Garcia, A.
Deposit date:2018-08-21
Release date:2018-10-24
Last modified:2018-12-19
Method:X-RAY DIFFRACTION (1.459144 Å)
Cite:Characterization of the catalytic flexible loop in the dihydroorotase domain of the human multi-enzymatic protein CAD.
J. Biol. Chem., 293, 2018
6HFL
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HUMAN DIHYDROOROTASE MUTANT F1563L CO-CRYSTALLIZED WITH CARBAMOYL ASPARTATE AT PH 7.0
Descriptor:CAD protein, ZINC ION, (4S)-2,6-DIOXOHEXAHYDROPYRIMIDINE-4-CARBOXYLIC ACID, ...
Authors:Ramon-Maiques, S., Grande Garcia, A.
Deposit date:2018-08-21
Release date:2018-10-24
Last modified:2018-12-19
Method:X-RAY DIFFRACTION (1.35 Å)
Cite:Characterization of the catalytic flexible loop in the dihydroorotase domain of the human multi-enzymatic protein CAD.
J. Biol. Chem., 293, 2018
6HFN
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HUMAN DIHYDROOROTASE MUTANT F1563L CO-CRYSTALLIZED WITH CARBAMOYL ASPARTATE AT PH 7.5
Descriptor:CAD protein, ZINC ION, FORMIC ACID, ...
Authors:Ramon-Maiques, S., Grande Garcia, A.
Deposit date:2018-08-21
Release date:2018-10-24
Last modified:2018-12-19
Method:X-RAY DIFFRACTION (1.449651 Å)
Cite:Characterization of the catalytic flexible loop in the dihydroorotase domain of the human multi-enzymatic protein CAD.
J. Biol. Chem., 293, 2018
6HFP
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HUMAN DIHYDROOROTASE MUTANT F1563T CO-CRYSTALLIZED WITH CARBAMOYL ASPARTATE AT PH 7.0
Descriptor:CAD protein, ZINC ION, (4S)-2,6-DIOXOHEXAHYDROPYRIMIDINE-4-CARBOXYLIC ACID, ...
Authors:Ramon-Maiques, S., Grande Garcia, A.
Deposit date:2018-08-21
Release date:2018-10-24
Last modified:2018-12-19
Method:X-RAY DIFFRACTION (1.198229 Å)
Cite:Characterization of the catalytic flexible loop in the dihydroorotase domain of the human multi-enzymatic protein CAD.
J. Biol. Chem., 293, 2018
6HFQ
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HUMAN DIHYDROOROTASE MUTANT F1563T CO-CRYSTALLIZED WITH CARBAMOYL ASPARTATE AT PH 7.5
Descriptor:CAD protein, ZINC ION, (4S)-2,6-DIOXOHEXAHYDROPYRIMIDINE-4-CARBOXYLIC ACID, ...
Authors:Ramon-Maiques, S., Grande Garcia, A.
Deposit date:2018-08-21
Release date:2018-10-24
Last modified:2018-12-19
Method:X-RAY DIFFRACTION (1.145425 Å)
Cite:Characterization of the catalytic flexible loop in the dihydroorotase domain of the human multi-enzymatic protein CAD.
J. Biol. Chem., 293, 2018
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