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1BVZ
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ALPHA-AMYLASE II (TVAII) FROM THERMOACTINOMYCES VULGARIS R-47
Descriptor: PROTEIN (ALPHA-AMYLASE II)
Authors:Kamitori, S, Kondo, S, Okuyama, K, Yokota, T, Shimura, Y, Tonozuka, T, Sakano, Y.
Deposit date:1998-09-22
Release date:1999-03-02
Last modified:2024-02-07
Method:X-RAY DIFFRACTION (2.6 Å)
Cite:Crystal structure of Thermoactinomyces vulgaris R-47 alpha-amylase II (TVAII) hydrolyzing cyclodextrins and pullulan at 2.6 A resolution.
J.Mol.Biol., 287, 1999
3D3I
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Crystal structural of Escherichia coli K12 YgjK, a glucosidase belonging to glycoside hydrolase family 63
Descriptor: CALCIUM ION, GLYCEROL, Uncharacterized protein ygjK
Authors:Kurakata, Y, Uechi, A, Yoshida, H, Kamitori, S, Sakano, Y, Nishikawa, A, Tonozuka, T.
Deposit date:2008-05-12
Release date:2008-06-03
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.78 Å)
Cite:Structural insights into the substrate specificity and function of Escherichia coli K12 YgjK, a glucosidase belonging to the glycoside hydrolase family 63.
J.Mol.Biol., 381, 2008
1G1Y
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CRYSTAL STRUCTURE OF ALPHA-AMYLASE II (TVAII) FROM THERMOACTINOMYCES VULGARIS R-47 AND BETA-CYCLODEXTRIN COMPLEX
Descriptor: ALPHA-AMYLASE II, Cycloheptakis-(1-4)-(alpha-D-glucopyranose)
Authors:Kondo, S, Ohtaki, A, Tonozuka, T, Sakano, Y, Kamitori, S.
Deposit date:2000-10-16
Release date:2001-03-14
Last modified:2024-02-07
Method:X-RAY DIFFRACTION (3 Å)
Cite:Studies on the hydrolyzing mechanism for cyclodextrins of Thermoactinomyces vulgaris R-47 alpha-amylase 2 (TVAII). X-ray structure of the mutant E354A complexed with beta-cyclodextrin, and kinetic analyses on cyclodextrins.
J.Biochem.(Tokyo), 129, 2001
1JF6
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Crystal structure of thermoactinomyces vulgaris r-47 alpha-amylase mutant F286Y
Descriptor: ALPHA AMYLASE II, CALCIUM ION
Authors:Ohtaki, A, Kondo, S, Shimura, Y, Tonozuka, T, Sakano, Y, Kamitori, S.
Deposit date:2001-06-20
Release date:2002-05-22
Last modified:2021-11-10
Method:X-RAY DIFFRACTION (3.2 Å)
Cite:Role of Phe286 in the recognition mechanism of cyclomaltooligosaccharides (cyclodextrins) by Thermoactinomyces vulgaris R-47 alpha-amylase 2 (TVAII). X-ray structures of the mutant TVAIIs, F286A and F286Y, and kinetic analyses of the Phe286-replaced mutant TVAIIs
CARBOHYDR.RES., 334, 2001
1JF5
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CRYSTAL STRUCTURE OF THERMOACTINOMYCES VULGARIS R-47 ALPHA-AMYLASE 2 MUTANT F286A
Descriptor: ALPHA AMYLASE II, CALCIUM ION
Authors:Ohtaki, A, Kondo, S, Shimura, Y, Tonozuka, T, Sakano, Y, Kamitori, S.
Deposit date:2001-06-20
Release date:2002-05-22
Last modified:2021-11-10
Method:X-RAY DIFFRACTION (3.2 Å)
Cite:Role of Phe286 in the recognition mechanism of cyclomaltooligosaccharides (cyclodextrins) by Thermoactinomyces vulgaris R-47 alpha-amylase 2 (TVAII). X-ray structures of the mutant TVAIIs, F286A and F286Y, and kinetic analyses of the Phe286-replaced mutant TVAIIs
CARBOHYDR.RES., 334, 2001
1JIB
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Complex of Alpha-amylase II (TVA II) from Thermoactinomyces vulgaris R-47 with Maltotetraose Based on a Crystal Soaked with Maltohexaose.
Descriptor: NEOPULLULANASE, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose
Authors:Yokota, T, Tonozuka, T, Shimura, Y, Ichikawa, K, Kamitori, S, Sakano, Y.
Deposit date:2001-07-02
Release date:2001-07-25
Last modified:2023-10-25
Method:X-RAY DIFFRACTION (3.3 Å)
Cite:Structures of Thermoactinomyces vulgaris R-47 alpha-amylase II complexed with substrate analogues.
Biosci.Biotechnol.Biochem., 65, 2001
2Z8G
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Aspergillus niger ATCC9642 isopullulanase complexed with isopanose
Descriptor: 2-acetamido-2-deoxy-beta-D-glucopyranose, Isopullulanase, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-6)-beta-D-glucopyranose
Authors:Mizuno, M, Koide, A, Yamamura, A, Akeboshi, H, Yoshida, H, Kamitori, S, Sakano, Y, Nishikawa, A, Tonozuka, T.
Deposit date:2007-09-05
Release date:2007-12-18
Last modified:2024-04-03
Method:X-RAY DIFFRACTION (1.7 Å)
Cite:Crystal Structure of Aspergillus niger Isopullulanase, a Member of Glycoside Hydrolase Family 49
J.Mol.Biol., 376, 2008
1JI2
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Improved X-ray Structure of Thermoactinomyces vulgaris R-47 alpha-Amylase 2
Descriptor: ALPHA-AMYLASE II, CALCIUM ION
Authors:Kamitori, S, Abe, A, Ohtaki, A, Kaji, A, Tonozuka, T, Sakano, Y.
Deposit date:2001-06-28
Release date:2002-06-05
Last modified:2024-03-13
Method:X-RAY DIFFRACTION (2.3 Å)
Cite:Crystal structures and structural comparison of Thermoactinomyces vulgaris R-47 alpha-amylase 1 (TVAI) at 1.6 A resolution and alpha-amylase 2 (TVAII) at 2.3 A resolution.
J.Mol.Biol., 318, 2002
3W7T
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Escherichia coli K12 YgjK complexed with mannose
Descriptor: CALCIUM ION, MAGNESIUM ION, Uncharacterized protein YgjK, ...
Authors:Miyazaki, T, Kurakata, Y, Uechi, A, Yoshida, H, Kamitori, S, Sakano, Y, Nishikawa, A, Tonozuka, T.
Deposit date:2013-03-06
Release date:2013-04-03
Last modified:2023-11-08
Method:X-RAY DIFFRACTION (1.5 Å)
Cite:Structural insights into the substrate specificity and function of Escherichia coli K12 YgjK, a glucosidase belonging to the glycoside hydrolase family 63.
J.Mol.Biol., 381, 2008
3W7S
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Escherichia coli K12 YgjK complexed with glucose
Descriptor: CALCIUM ION, Uncharacterized protein YgjK, alpha-D-glucopyranose
Authors:Miyazaki, T, Kurakata, Y, Uechi, A, Yoshida, H, Kamitori, S, Sakano, Y, Nishikawa, A, Tonozuka, T.
Deposit date:2013-03-06
Release date:2013-04-03
Last modified:2020-07-29
Method:X-RAY DIFFRACTION (1.9 Å)
Cite:Structural insights into the substrate specificity and function of Escherichia coli K12 YgjK, a glucosidase belonging to the glycoside hydrolase family 63.
J.Mol.Biol., 381, 2008
3W7U
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Escherichia coli K12 YgjK complexed with galactose
Descriptor: CALCIUM ION, Uncharacterized protein YgjK, alpha-D-galactopyranose
Authors:Miyazaki, T, Kurakata, Y, Uechi, A, Yoshida, H, Kamitori, S, Sakano, Y, Nishikawa, A, Tonozuka, T.
Deposit date:2013-03-06
Release date:2013-04-03
Last modified:2020-07-29
Method:X-RAY DIFFRACTION (1.99 Å)
Cite:Structural insights into the substrate specificity and function of Escherichia coli K12 YgjK, a glucosidase belonging to the glycoside hydrolase family 63.
J.Mol.Biol., 381, 2008
1JL8
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Complex of alpha-amylase II (TVA II) from Thermoactinomyces vulgaris R-47 with beta-cyclodextrin based on a co-crystallization with methyl beta-cyclodextrin
Descriptor: ALPHA-AMYLASE II, Cycloheptakis-(1-4)-(alpha-D-glucopyranose)
Authors:Yokota, T, Tonozuka, T, Shimura, Y, Ichikawa, K, Kamitori, S, Sakano, Y.
Deposit date:2001-07-16
Release date:2001-08-01
Last modified:2023-10-25
Method:X-RAY DIFFRACTION (3.2 Å)
Cite:Structures of Thermoactinomyces vulgaris R-47 alpha-amylase II complexed with substrate analogues.
Biosci.Biotechnol.Biochem., 65, 2001
2D0H
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Crystal Structure of Thermoactinomyces vulgaris R-47 Alpha-Amylase 1 (TVAI) Mutant D356N/E396Q complexed with P2, a pullulan model oligosaccharide
Descriptor: (4S)-2-METHYL-2,4-PENTANEDIOL, CALCIUM ION, alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, ...
Authors:Abe, A, Yoshida, H, Tonozuka, T, Sakano, Y, Kamitori, S.
Deposit date:2005-08-02
Release date:2006-07-11
Last modified:2021-11-10
Method:X-RAY DIFFRACTION (2.1 Å)
Cite:Complexes of Thermoactinomyces vulgaris R-47 alpha-amylase 1 and pullulan model oligossacharides provide new insight into the mechanism for recognizing substrates with alpha-(1,6) glycosidic linkages
Febs J., 272, 2005
2D0F
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Crystal Structure of Thermoactinomyces vulgaris R-47 Alpha-Amylase 1 (TVAI) Mutant D356N complexed with P2, a pullulan model oligosaccharide
Descriptor: (4S)-2-METHYL-2,4-PENTANEDIOL, CALCIUM ION, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-4)-beta-D-glucopyranose, ...
Authors:Abe, A, Yoshida, H, Tonozuka, T, Sakano, Y, Kamitori, S.
Deposit date:2005-08-02
Release date:2006-07-11
Last modified:2021-11-10
Method:X-RAY DIFFRACTION (2.08 Å)
Cite:Complexes of Thermoactinomyces vulgaris R-47 alpha-amylase 1 and pullulan model oligossacharides provide new insight into the mechanism for recognizing substrates with alpha-(1,6) glycosidic linkages
Febs J., 272, 2005
2D0G
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Crystal Structure of Thermoactinomyces vulgaris R-47 Alpha-Amylase 1 (TVAI) Mutant D356N/E396Q complexed with P5, a pullulan model oligosaccharide
Descriptor: (4S)-2-METHYL-2,4-PENTANEDIOL, CALCIUM ION, alpha-D-glucopyranose, ...
Authors:Abe, A, Yoshida, H, Tonozuka, T, Sakano, Y, Kamitori, S.
Deposit date:2005-08-02
Release date:2006-07-11
Last modified:2021-11-10
Method:X-RAY DIFFRACTION (2.6 Å)
Cite:Complexes of Thermoactinomyces vulgaris R-47 alpha-amylase 1 and pullulan model oligossacharides provide new insight into the mechanism for recognizing substrates with alpha-(1,6) glycosidic linkages
Febs J., 272, 2005
2D2O
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Structure of a complex of Thermoactinomyces vulgaris R-47 alpha-amylase 2 with maltohexaose demonstrates the important role of aromatic residues at the reducing end of the substrate binding cleft
Descriptor: CALCIUM ION, Neopullulanase 2, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose
Authors:Ohtaki, A, Mizuno, M, Yoshida, H, Tonozuka, T, Sakano, Y, Kamitori, S.
Deposit date:2005-09-13
Release date:2006-08-29
Last modified:2021-11-10
Method:X-RAY DIFFRACTION (2.1 Å)
Cite:Structure of a complex of Thermoactinomyces vulgaris R-47 alpha-amylase 2 with maltohexaose demonstrates the important role of aromatic residues at the reducing end of the substrate binding cleft
Carbohydr.Res., 341, 2006
2ZYK
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Crystal structure of cyclo/maltodextrin-binding protein complexed with gamma-cyclodextrin
Descriptor: Cyclooctakis-(1-4)-(alpha-D-glucopyranose), Solute-binding protein
Authors:Tonozuka, T, Sogawa, A, Yamada, M, Matsumoto, N, Yoshida, H, Kamitori, S, Ichikawa, K, Mizuno, M, Nishikawa, A, Sakano, Y.
Deposit date:2009-01-26
Release date:2009-02-10
Last modified:2024-04-03
Method:X-RAY DIFFRACTION (2.5 Å)
Cite:Structural basis for cyclodextrin recognition by Thermoactinomyces vulgaris cyclo/maltodextrin-binding protein
Febs J., 274, 2007
1IZJ
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Thermoactinomyces vulgaris R-47 alpha-amylase 1 mutant enzyme f313a
Descriptor: CALCIUM ION, amylase
Authors:Ohtaki, A, Iguchi, A, Mizuno, M, Tonozuka, T, Sakano, Y, Kamitori, S.
Deposit date:2002-10-03
Release date:2003-07-29
Last modified:2023-12-27
Method:X-RAY DIFFRACTION (2.2 Å)
Cite:Mutual conversion of substrate specificities of Thermoactinomyces vulgaris R-47 alpha-amylases TVAI and TVAII by site-directed mutagenesis
CARBOHYDR.RES., 338, 2003
1IZK
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Thermoactinomyces vulgaris R-47 alpha-amylase 1 mutant enzyme w398v
Descriptor: CALCIUM ION, amylase
Authors:Ohtaki, A, Iguchi, A, Mizuno, M, Tonozuka, T, Sakano, Y, Kamitori, S.
Deposit date:2002-10-03
Release date:2003-07-29
Last modified:2023-12-27
Method:X-RAY DIFFRACTION (2.2 Å)
Cite:Mutual conversion of substrate specificities of Thermoactinomyces vulgaris R-47 alpha-amylases TVAI and TVAII by site-directed mutagenesis
CARBOHYDR.RES., 338, 2003
1JI1
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Crystal Structure Analysis of Thermoactinomyces vulgaris R-47 alpha-Amylase 1
Descriptor: ALPHA-AMYLASE I, CALCIUM ION
Authors:Kamitori, S, Abe, A, Ohtaki, A, Kaji, A, Tonozuka, T, Sakano, Y.
Deposit date:2001-06-28
Release date:2002-06-05
Last modified:2024-03-13
Method:X-RAY DIFFRACTION (1.6 Å)
Cite:Crystal structures and structural comparison of Thermoactinomyces vulgaris R-47 alpha-amylase 1 (TVAI) at 1.6 A resolution and alpha-amylase 2 (TVAII) at 2.3 A resolution.
J.Mol.Biol., 318, 2002
1WZL
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Thermoactinomyces vulgaris R-47 alpha-amylase II (TVA II) mutatnt R469L
Descriptor: Alpha-amylase II, CALCIUM ION
Authors:Mizuno, M, Ichikawa, K, Tonozuka, T, Ohtaki, A, Shimura, Y, Kamitori, S, Nishikawa, A, Sakano, Y.
Deposit date:2005-03-06
Release date:2005-03-22
Last modified:2021-11-10
Method:X-RAY DIFFRACTION (2 Å)
Cite:Mutagenesis and Structural Analysis of Thermoactinomyces vulgaris R-47 alpha-Amylase II (TVA II)
To be Published
1WZK
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Thermoactinomyces vulgaris R-47 alpha-amylase II (TVA II) mutatnt D465N
Descriptor: Alpha-amylase II, CALCIUM ION
Authors:Mizuno, M, Ichikawa, K, Tonozuka, T, Ohtaki, A, Shimura, Y, Kamitori, S, Nishikawa, A, Sakano, Y.
Deposit date:2005-03-06
Release date:2005-03-22
Last modified:2021-11-10
Method:X-RAY DIFFRACTION (2.3 Å)
Cite:Mutagenesis and Structural Analysis of Thermoactinomyces vulgaris R-47 alpha-Amylase II (TVA II)
To be Published
1WZM
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Thermoactinomyces vulgaris R-47 alpha-amylase II (TVA II) mutatnt R469K
Descriptor: Alpha-amylase II, CALCIUM ION
Authors:Mizuno, M, Ichikawa, K, Tonozuka, T, Ohtaki, A, Shimura, Y, Kamitori, S, Nishikawa, A, Sakano, Y.
Deposit date:2005-03-06
Release date:2005-03-22
Last modified:2021-11-10
Method:X-RAY DIFFRACTION (3.2 Å)
Cite:Mutagenesis and Structural Analysis of Thermoactinomyces vulgaris R-47 alpha-Amylase II (TVA II)
To be Published
1WMR
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Crystal Structure of Isopullulanase from Aspergillus niger ATCC 9642
Descriptor: 2-acetamido-2-deoxy-beta-D-glucopyranose, Isopullulanase
Authors:Mizuno, M, Tonozuka, T, Miyasaka, Y, Akeboshi, H, Kamitori, S, Nishikawa, A, Sakano, Y.
Deposit date:2004-07-15
Release date:2005-07-19
Last modified:2023-10-25
Method:X-RAY DIFFRACTION (2.4 Å)
Cite:Crystal Structure of Aspergillus niger Isopullulanase, a Member of Glycoside Hydrolase Family 49
J.Mol.Biol., 376, 2008
1X0C
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Improved Crystal Structure of Isopullulanase from Aspergillus niger ATCC 9642
Descriptor: 2-acetamido-2-deoxy-beta-D-glucopyranose, Isopullulanase
Authors:Mizuno, M, Tonozuka, T, Yamamura, A, Miyasaka, Y, Akeboshi, H, Kamitori, S, Nishikawa, A, Sakano, Y.
Deposit date:2005-03-17
Release date:2006-06-13
Last modified:2020-07-29
Method:X-RAY DIFFRACTION (1.7 Å)
Cite:Crystal Structure of Aspergillus niger Isopullulanase, a Member of Glycoside Hydrolase Family 49
J.Mol.Biol., 376, 2008

 

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